Stirred cell membrane emulsification for multiple emulsions containing unrefined pumpkin seed oil with uniform droplet size

Dragosavac, Marijana M.; Holdich, Richard; Vladisavljević, Goran T.; Sovilj, Milan N.

doi:10.1016/j.memsci.2011.12.009

Cited by 53 publications

(54 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2(a), it is shown that D av decreases with increasing shear stress. This trend is in accordance with data reported previously [29,37,42]. Modifying the shear stress applied it was possible to produce droplets sized between 60 µm to 340 µm using the 30 µm membrane.…”

Section: Influence Of Emulsification Process Parameters and Cells Presupporting

confidence: 92%

“…It uses low shear conditions and requires low energy input [38,41]. ME is easier to scale up and has a higher productivity compared to the other "drop-by-drop" methods of emulsion production (microchannel and microfluidic devices) [42]. All these characteristics make ME particularly advantageous for the encapsulation of shear sensitive, temperature sensitive and delicate compounds such as drugs, proteins and cells, provided that it can be shown that the membrane used for the emulsification does not filter out the cells to be encapsulated.…”

Section: Introductionmentioning

confidence: 99%

“…The lack of a tortuous pore channel minimizes membrane fouling, or cell filtration, during the process of encapsulation. In this work the Dispersion Cell Membrane Emulsification device [29,37,40,42] is used for the production of W/O emulsions containing yeast cells using micro-sieve type metal membranes made hydrophobic by a process of surface coating. The performance of hydrophobic membranes coated with different methods (PTFE and FAS fluorinated compounds) is shown.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Morelli

Holdich

Dragosavac

2017

Journal of Membrane Science

View full text Add to dashboard Cite

Citation: MORELLI, S., HOLDICH, R. and DRAGOSAVAC, M., 2017. Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification. Journal of Membrane Science, 524, Additional Information:• This paper was accepted for publication in the journal Journal of This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. conditions. The liquid drops were loaded with increasing amount of yeast (3.14 x 10 7 to 3.14 x 10 8 cells/ mL). The stability and uniformity of the emulsions was independent of the cell concentration. PTFE coated hydrophobic membrane produced smaller W/O drops compared to FAS coated membranes. The liquid polymeric droplets were solidified in solid particles using thermal gelation and/or ionic crosslinking, obtaining yeast encapsulated particles sized ~100 µm.The pH sensitive polymer, Eudragit S100, was used as a coating to create gastro resistant particles suitable for intestinal-colonic targeted release. Viability of the released yeast cells was demonstrated using fluorescence probes and checking cell glucose metabolism with time. AbbreviationsW/O emulsion, water in oil

show abstract

Section: Influence Of Emulsification Process Parameters and Cells Presupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Morelli

Holdich

Dragosavac

2017

Journal of Membrane Science

View full text Add to dashboard Cite

show abstract

“…A similar linear relation between the particle size and pore size of microengineered membrane was obtained in fabrication of liposomes 39 and membrane emulsification. [40][41] The results clearly show that the micelle size can be controlled by the membrane pore size. The size uniformity increased with decreasing the pore size ( Figure 6b).…”

Section: Resultsmentioning

confidence: 99%

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Laouini

Koutroumanis

Charcosset

et al. 2013

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

A novel membrane contactor method was used to produce size-controlled poly(ethylene glycol)-b-polycaprolactone (PEG-PCL) copolymer micelles composed of diblock copolymers with different average molecular weights, M n (9200 or 10 400 Da) and hydrophilic fractions, f (0.67 or 0.59). By injecting 570 L m −2 h −1 of the organic phase (a 1 mg mL −1 solution of PEG-PCL in tetrahydrofuran) through a microengineered nickel membrane with a hexagonal pore array and 200 μm pore spacing into deionized water agitated at 700 rpm, the micelle size linearly increased from 92 nm for a 5-μm pore size to 165 nm for a 40-μm pore size. The micelle size was finely tuned by the agitation rate, transmembrane flux and aqueous to organic phase ratio. An encapsulation efficiency of 89% and a drug loading of ∼75% (w/w) were achieved when a hydrophobic drug (vitamin E) was entrapped within the micelles, as determined by ultracentrifugation method. The drug-loaded micelles had a mean size of 146 ± 7 nm, a polydispersity index of 0.09 ± 0.01, and a ζ potential of −19.5 ± 0.2 mV. When drug-loaded micelles where stored for 50 h, a pH sensitive drug release was achieved and a maximum amount of vitamin E (23%) was released at the pH of 1.9. When a pH-sensitive hydrazone bond was incorporated between PEG and PCL blocks, no significant change in micelle size was observed at the same micellization conditions.

show abstract

“…Using swirl-flow ME with a Shirasu Porous Glass (SPG) membrane, it was possible to achieve a high transmembrane flux of 3 m 3 m -2 h -1 resulting in a dispersed phase volume of 40% in a single pass, but at the expense of high shear stress at the membrane surface. ME is often used for production of multiple emulsions 7 and fragile particles, such as core/shell capsules 8, 9 . In order to prevent breakage of shear sensitive materials, it is highly desirable to maintain low shear conditions in the bulk of the product stream, although shear stress at the membrane surface may be quite significant.…”

Section: Introductionmentioning

confidence: 99%

Continuous Membrane Emulsification with Pulsed (Oscillatory) Flow

Holdich¹,

Dragosavac²,

Vladisavljević³

et al. 2013

Ind. Eng. Chem. Res.

Self Cite

View full text Add to dashboard Cite

Tubular micron pore sized sieve type membranes with internal diameter of 14 mm and length of 60 mm containing uniform pores of diameter 10 and 20 µm were used to generate emulsions of sunflower oil dispersed in water and stabilised by Tween 20 using oscillatory flow of the continuous phase. Drop diameters between 30 and 300 µm could be produced, in a controllable way and with span values of down to 0.4. By using pulsed flow it was possible to provide dispersed phase concentrations of up to 45% v/v in a single pass over the membrane, i.e. without the need to recirculate the continuous phase through the membrane tube. It was possible to correlate the drop size produced with the shear conditions at the membrane surface using the wave shear stress equation. The oscillatory Reynolds number indicated flow varying from laminar to substantially turbulent, but the change in flow conditions did not show a notable influence on the drop diameters produced, over what is predicted by the varying wall shear stress applied to the wave equation. However, the 20 µm pore sized sieve type membrane appeared to allow the passage of the pressure pulse through the membrane pores, under certain operating conditions, which did lead to finer drop sizes produced than would be predicted. These through-membrane pulsations could be suppressed by changes in operating conditions: a higher dispersed phase injection rate or more viscous continuous phase, and they did not occur under similar operating conditions used with the 10 2 µm pore sized sieve type of membrane. Generating emulsions of this size using pulsed continuous phase flow provides opportunities for combining drop generation at high dispersed phase concentration, by membrane emulsification, with downstream processing such as reaction in plug flow reactors.

show abstract

Stirred cell membrane emulsification for multiple emulsions containing unrefined pumpkin seed oil with uniform droplet size

Cited by 53 publications

References 33 publications

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Continuous Membrane Emulsification with Pulsed (Oscillatory) Flow

Contact Info

Product

Resources

About