StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato

Zhou, Xiangjun; Zha, Manrong; Huang, Jing; Li, Li; Imran, Muhammad; Zhang, Cankui

doi:10.1093/jxb/erx026

Cited by 83 publications

(59 citation statements)

References 66 publications

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“…pRI 101-AN was used as the binary vector. Transgenic potato plants were produced by Agrobacterium tumefaciens-mediated transformation following the protocol described by Zhou et al (2017). Grafting transgenic potato onto wild-type potato followed the same procedure described above.…”

Section: Grafting and Tissue Samplingmentioning

confidence: 99%

Elucidation of the Mechanisms of Long-Distance mRNA Movement in aNicotiana benthamiana/Tomato Heterograft System

et al. 2018

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Recent heterograft analyses showed that large-scale messenger RNA (mRNA) movement takes place in the phloem, but the number of mobile transcripts reported varies widely. However, our knowledge of the mechanisms underlying large-scale mRNA movement remains limited. In this study, using a /tomato () heterograft system and a transgenic approach involving potato (), we found that: (1) the overall mRNA abundance in the leaf is not a good indicator of transcript mobility to the root; (2) increasing the expression levels of nonmobile mRNAs in the companion cells does not promote their mobility; (3) mobile mRNAs undergo degradation during their movement; and (4) some mRNAs arriving in roots move back to shoots. These results indicate that mRNA movement has both regulated and unregulated components. The cellular origins of mobile mRNAs may differ between herbaceous and woody species. Taken together, these findings suggest that the long-distance movement of mRNAs is a complex process and that elucidating the physiological roles associated with this movement is challenging but remains an important task for future research.

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Section: Grafting and Tissue Samplingmentioning

confidence: 99%

Elucidation of the Mechanisms of Long-Distance mRNA Movement in aNicotiana benthamiana/Tomato Heterograft System

et al. 2018

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“…MYB44 gene is highly downregulated under low-Pi in roots [123], which may be the result of our identified putative miRNA's guided cleavage. Studies in potato have indicated that MYB44 TF may form a regulatory complex together with WRKY6 TF, which negatively regulates Pi transport by suppressing PHO1 expression [123]. Other degradome records, among the most differentially expressed sRNAs, were found to target mRNAs of the V-ATPase assembly factor (VMA21-like) and three barley genomic loci encoding uncharacterized proteins (HORVU7Hr1G053570, HORVU1Hr1G027340, and HORVU0Hr1G023910) (Fig.…”

Section: Degradome Profiling Describes Post-transcriptional Regulatormentioning

confidence: 86%

Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses

Sega

Kruszka

Bielewicz

et al. 2020

Preprint

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Background: Small RNAs (sRNAs) are 18–24 nt regulatory elements which are responsible for plant development regulation and participate in many plant stress responses. Insufficient inorganic phosphate (Pi) concentration triggers plant responses to balance the internal Pi level. Results: In this study, we describe Pi-starvation-responsive small RNAs and transcriptome changes in barley (Hordeum vulgare L.) using Next-Generation Sequencing (NGS) data derived from three different types of NGS libraries: (i) small RNAs, (ii) degraded RNAs, and (iii) functional mRNAs. We find that differentially and significantly expressed miRNAs (DEMs, p-value < 0.05) are represented by 162 (44.88 % of total differentially expressed small RNAs) molecules in shoot and 138 (7.14 %) in root; mainly various miR399 and miR827 isomiRs. The remaining small RNAs (i.e., those without perfect match to reference sequences deposited in miRBase) are considered as differentially expressed other sRNAs (DESs, Bonferroni correction). In roots, a more abundant and diverse set of other sRNAs (1796 unique sequences, 0.13 % from total unique reads obtained under low-Pi) contributes more to the compensation of low-Pi stress than that in shoots (199 unique sequences, 0.01 %). More than 80 % of differentially expressed other sRNAs are upregulated in both organs. Additionally, in barley shoots, upregulation of small RNAs is accompanied by strong induction of two nucleases (S1/P1 endonuclease and 3’-5’ exonuclease). This suggests that most small RNAs may be generated upon endonucleolytic cleavage to increase the internal Pi pool. Transcriptomic profiling of Pi-starved barley shoots identify 98 differentially expressed genes (DEGs). A majority of the DEGs possess characteristic Pi-responsive cis-regulatory elements (P1BS and/or PHO element), located mostly in the proximal promoter regions. GO analysis shows that the discovered DEGs primarily alter plant defense, plant stress response, nutrient mobilization, or pathways involved in the gathering and recycling of phosphorus from organic pools.Conclusions: Our results provide comprehensive data to demonstrate complex responses at the RNA level in barley to maintain Pi homeostasis and indicate that barley adapts to Pi scarcity through elicitation of RNA degradation. Novel P-responsive genes were selected as putative candidates to overcome low-Pi stress in barley plants.

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“…For example, in roots, the highly up-regulated 20 nt sequence 5'-ACCGACCTACTTGACCCTTC-3' (log 2 (fc) = 6.46, id = 348) binds to the 3'-UTR region of the MYB44 TF's mRNA and guides/promotes cleavage in the 1037 position (PAREsnip2: score = 4; MFE = -33.3) ( Table 2). RNA-Seq data for potato (Solanum tuberosum L.) proved that expression of the MYB44 gene is highly downregulated under low-Pi in roots [98], which may be the result of miRNA-guided PTGS. Studies in potato have indicated that MYB44 TF may form a regulatory complex together with WRKY6 TF, which negatively regulates Pi transport by suppressing PHO1 expression [98].…”

Section: Pi-responsive Motifs Found In the Promoters Of Degsmentioning

confidence: 99%

“…RNA-Seq data for potato (Solanum tuberosum L.) proved that expression of the MYB44 gene is highly downregulated under low-Pi in roots [98], which may be the result of miRNA-guided PTGS. Studies in potato have indicated that MYB44 TF may form a regulatory complex together with WRKY6 TF, which negatively regulates Pi transport by suppressing PHO1 expression [98]. Other degradome records, among the most differentially expressed sRNAs, were found to target mRNAs of the V-ATPase assembly factor (VMA21-like) and three barley genomic loci encoding uncharacterized proteins (HORVU7Hr1G053570, HORVU1Hr1G027340, and HORVU0Hr1G023910) ( Table 2).…”

Section: Pi-responsive Motifs Found In the Promoters Of Degsmentioning

confidence: 99%

Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses

Sega

Kruszka

Bielewicz

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Small RNAs (sRNAs) are 20–30 nt regulatory elements which are responsible for plant development regulation and participate in many plant stress responses. Insufficient inorganic phosphate (Pi) concentration triggers plant responses to balance the internal Pi level. Results: In this study, we describe Pi-starvation-responsive small RNAs and transcriptome changes in barley (Hordeum vulgare L.) using Next-Generation Sequencing (NGS) RNA-Seq data derived from three different types of NGS libraries: (i) small RNAs, (ii) degraded RNAs, and (iii) functional mRNAs. We find that differentially and significantly expressed miRNAs (DEMs, p-value < 0.05) are represented by 162 (44.88 % of total differentially expressed small RNAs) molecules in shoot and 138 (7.14 %) in root; mainly various miR399 and miR827 isomiRs. The remaining small RNAs (i.e., those without perfect match to reference sequences deposited in miRBase) are considered as differentially expressed other sRNAs (DESs, p-value Bonferroni correction < 0.05). In roots, a more abundant and diverse set of other sRNAs (DESs, 1796 unique sequences, 0.13 % from total unique reads obtained under low-Pi) contributes more to the compensation of low-Pi stress than that in shoots (DESs, 199 unique sequences, 0.01 %). More than 80 % of differentially expressed other sRNAs are up-regulated in both organs. Additionally, in barley shoots, up-regulation of small RNAs is accompanied by strong induction of two nucleases (S1/P1 endonuclease and 3’-5’ exonuclease). This suggests that most small RNAs may be generated upon endonucleolytic cleavage to increase the internal Pi pool. Transcriptomic profiling of Pi-starved barley shoots identifies 98 differentially expressed genes (DEGs). A majority of the DEGs possess characteristic Pi-responsive cis-regulatory elements (P1BS and/or PHO element), located mostly in the proximal promoter regions. GO analysis shows that the discovered DEGs primarily alter plant defense, plant stress response, nutrient mobilization, or pathways involved in the gathering and recycling of phosphorus from organic pools.Conclusions: Our results provide comprehensive data to demonstrate complex responses at the RNA level in barley to maintain Pi homeostasis and indicate that barley adapts to Pi-starvation through elicitation of RNA degradation. Novel P-responsive genes were selected as putative candidates to overcome low-Pi stress in barley plants.

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StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato

Abstract: HighlightPi deficiency suppressed the transcription factor gene StMYB44, encoding the protein that interacts with Arabidopsis WRKY6 and StWRKY6 and negatively affects Pi distribution by suppressing the expression of PHOSPHATE1 in potato.

Cited by 83 publications

References 66 publications

Elucidation of the Mechanisms of Long-Distance mRNA Movement in aNicotiana benthamiana/Tomato Heterograft System

Elucidation of the Mechanisms of Long-Distance mRNA Movement in aNicotiana benthamiana/Tomato Heterograft System

Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses

Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses

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