Stoichiometry and kinetics of the PHB-producing Type II methanotrophs Methylosinus trichosporium OB3b and Methylocystis parvus OBBP

Review of Sustainable Methane Mitigation and Biopolymer Production

“…Maximal PHA/PHB accumulation of 36-70% has been reported for Methylocystis parvus OBBP, while only 7-9% was reported for Methylocystis hirsute CSC 1 (Asenjo and Suk, 1986;Pieja et al, 2011a;Rostkowski et al, 2013). Methylosinus and Methylobacterium were the other two genera reported to have fairly high amounts of PHB (Bourque et al, 1995;Kim et al, 1996;Zhang et al, 2008;Doronina et al, 2008; (Zhang et al, 2008;Zuniga et al, 2011;Xin et al, 2011;Rostkowski et al, 2013). Differences in reported PHA/PHB storage capacities may be attributable to varying physical and environmental factors (detailed in Section 3) applied during batch/continuous culture experiments.…”

“…PHA/PHB accumulation potential of pure and mixed cultures of methanotrophs under universally optimized conditions is thus poorly documented in the literature (Table 3). The genus Methylocystis dominates mixed species consortia in different environments and is therefore perhaps the most commonly studied genus (Helm et al, 2006;Peija et al, 2011;Rahnama et al, 2012;Rostkowski et al, 2013). Maximal PHA/PHB accumulation of 36-70% has been reported for Methylocystis parvus OBBP, while only 7-9% was reported for Methylocystis hirsute CSC 1 (Asenjo and Suk, 1986;Pieja et al, 2011a;Rostkowski et al, 2013).…”

“…The genus Methylocystis dominates mixed species consortia in different environments and is therefore perhaps the most commonly studied genus (Helm et al, 2006;Peija et al, 2011;Rahnama et al, 2012;Rostkowski et al, 2013). Maximal PHA/PHB accumulation of 36-70% has been reported for Methylocystis parvus OBBP, while only 7-9% was reported for Methylocystis hirsute CSC 1 (Asenjo and Suk, 1986;Pieja et al, 2011a;Rostkowski et al, 2013). Methylosinus and Methylobacterium were the other two genera reported to have fairly high amounts of PHB (Bourque et al, 1995;Kim et al, 1996;Zhang et al, 2008;Doronina et al, 2008; (Zhang et al, 2008;Zuniga et al, 2011;Xin et al, 2011;Rostkowski et al, 2013).…”

“…In addition, they are non-toxic and biocompatible for a wide range of medical applications (Wendlandt et al, 2010). Therefore, research interest in these directions is developing and CH 4 emitted from anthropogenic sources is being considered as one of the ideal carbon sources for biopolymer production (Asenjo et al, 1986;Kim et al, 1996;Wendlandt et al, 2001;Helm et al, 2006;Listewnik et al, 2007;Doronina et al, 2008;Dong et al, 2011;Zhang et al, 2011;Zuniga et al, 2011;Pieja et al, 2012;Khosravi-darni et al, 2013;Rostkowski et al, 2013). Methane to biopolymer conversion provides a common solution for three major environmental problems, viz., (1) a potential approach for carbon sequestration and GHGs emission reduction; (2) production of biodegradable polymers for replacing conventional fossil fuel-derived plastics; and (3) reducing the use of organic carbon sources such as sugars for PHA/PHB production.…”

Effect of storage on the respirometric relationship between substrate utilization and microbial growth

“…e l s e v i e r . c o m / l o c a t e / b i o r t e c h growth rate, l max , to calculate the maximum specific rate of substrate utilization (Rostkowski et al, 2013).…”