Stone cell distribution and lignin structure in various pear varieties

Yan, Chongchong; Mei, Yuxia; Zhang, Nan; Jin, Qing; Zhi, Fang; Lin, Yi; Cai, Yongping

doi:10.1016/j.scienta.2014.05.018

Cited by 81 publications

(67 citation statements)

References 10 publications

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“…This acts as a type of sclerenchymatous cells in the pear pulp, which determines the internal quality of pear fruits (Cai et al, 2010;Jin et al, 2013). An increased concentration of stone cells in pear fruit leads to a muricated taste, poor edible quality, and loss of economic value (Choi and Lee, 2013;Zhao et al, 2013;Yan et al, 2014). During the formation of stone cells, lignin is deposited in the corner of the primary wall of parenchyma cells, from where it extends to the intercellular and secondary wall layers along the primary wall corner until the whole cell lumen is covered.…”

Section: Introductionmentioning

confidence: 99%

“…The results of previous studies have shown that PbCCOMT genes are expressed at high levels during the early stages of fruit development, which might promote the accumulation of pear stone cells (Wu et al, 2012). Furthermore, a higher ratio of G units can lead large trouble in degradation of polymeride lignin, which subsequently increases the degree of sclereid polymerization, and seriously affects the quality and taste of pear fruit (Yan et al, 2014). This indicates that CCOMT is a valuable regulatory gene.…”

Section: Introductionmentioning

confidence: 99%

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Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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ABSTRACT. With high nutritional value in its fruits, Dangshan Su pear has been widely cultivated in China. The stone cell content in fruits is a key factor affecting fruit quality in pear, and the formation of stone cells has been associated with lignin biosynthesis. O-Methyltransferase (OMT) is a key enzyme involved in lignin metabolism within the phenylpropanoid pathway. Here, we screened 26 OMT genes from the Pyrus bretschneideri cv. Dangshan Su genome using the DNATOOLs software. To characterize the OMT gene family in pear, gene structure, chromosomal localization, and conserved motifs of PbOMTs were analyzed. PbOMTs were divided into two categories, type I (designated PbCCOMTs) and type II (designated PbCOMTs), indicating the differentiation of function during evolution. Based on the analysis of multiple sequence alignment, cis-element prediction, and phylogenetic relationships, two candidate genes, PbCCOMT1 and PbCCOMT3, were selected for the analysis of temporal and spatial gene expression in pear. The promoter regions of both PbCCOMT1 and PbCCOMT3 contain regulatory motifs for lignin synthesis. Moreover, the two genes show high similarity and close phylogenetic relationships with CCOMTs in other species. Expression analysis showed that transcript levels of two PbCCOMTs were positively associated with the contents of both stone cells and lignin during the development of pear fruit. These results suggest that PbCCOMT1 and PbCCOMT3 are closely associated with lignin biosynthesis. These findings will help clarify the function of PbOMTs in lignin metabolism and to elucidate the mechanisms underlying stone cell formation in pear.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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“…However, the expression levels of genes positively regulated with lignin content were universally much higher than that of negatively regulated genes, indicating that they are the core genes responsible for lignin synthesis during stone cell formation in pear fruits. Genes and enzymes involved in monolignol biosynthesis have been studied extensively in different pear varieties [1,2,8,21,[60][61] . Therefore roles of these genes during lignin accumulation in pear pulp will not be discussed in detail.…”

Section: Discussionmentioning

confidence: 99%

Integrated transcriptomic and proteomic analysis reveal new insights into stone cell formation in Korla pear

Mamat¹,

Zhang²,

Xu³

et al. 2020

Preprint

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Background Korla fragrant pear(P•sinkiangensis Yü)is a famous local variety of Xinjiang China. One difficulty is the high stone cell content of these pears, which causes the formation of rough skins on the fruit. To elucidate the underlying mechanisms of stone cell formation, parallel analyses of the transcriptome and proteome was performed to identify important regulators and pathways involved in stone cell formation.Results Fruit samples were collected at three important time points depending on the stages of stone cell formation (20, 50 and 80 days after flowering). A total of 24268 differentially expressed genes (DEGs) and 1011 differentially accumulated proteins (DAPs) were identified from all the time points. Function analysis of the differential genes/proteins revealed that a set of candidates was associated with stone cell formation. These candidates mainly enriched in pathways involved in lignin biosynthesis, cellulose and xylan biosynthesis, S-adenosylmethionine (SAM) metabolic process, Reactive oxygen species (ROS) production, and cell death. We mined a total of 253 DEGs, and 100 DAPs, 63 of which were significantly changed at both the transcript and protein levels during fruit development.Conclusions Our findings reveal that some intriguing genes/proteins were previously unrecognized related with the sclereid formation, which provided new insights into molecular processes regulating sclereid accumulation in pear pulp.

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“…Compared with other genes, PbBGLU38 and PbBGLU43 are more uniformly expressed in all tissue parts and all stages, and may perform a certain indispensable function in pear fruit trees. The expression levels of PbBGLU28 and PbBGLU29 gene in owers, 79 DAF (when the ligni cation stop in fruit) [31,35,36] and 145 DAF (when the fruit was fully mature) [31,35,36] relatively high. Moreover, these two genes were clustered together with AtBGLU1-6 which related to the accumulation of avonoids in Arabidopsis [2], so PbBGLU28 and PbBGLU29 may participate in the accumulation of avonoids in pear.…”

Section: Expression Characteristics Of Chinese White Pear Bglu Genesmentioning

confidence: 99%

“…However, due to its high content of stone cells, its economic value is not high enough [31]. Stone cells are a kind of peculiar cell in pear fruit, it is one of the important factors that affect pear fruit processing and fresh food quality.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Comparative Analysis of the β-glucosidase Family in Five Rosaceae Species and their Potential Role on Lignification of Stone Cells in Chinese White Pear

Wang

Yang

Han

et al. 2020

Preprint

Self Cite

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Background: The β-glucosidase BGLU in the glycoside hydrolase family 1 (GH1) is involved in the sugar metabolism of the plant and plays an important role in maintaining the normal physiological function of the plant. Recent studies had shown that β-glucosidase was involved in plant lignification. The lignification in pear fruit is closely related to the formation of pear stone cells, but the BGLU genes family has not been identified in pears.Result: A total of 343 BGLU genes were identified from five species of Rosaceae (Pyrus bretschneideri, Prunus mume, Malus domestica, Prunus avium, Fragaria vesca). According to phylogenetic analysis, 50 PbBGLUs were divided into 8 groups. 298 syntenic pairs were found in intra- and inter-species collinear analysis of five Rosaceae species, found that pears and apples had more syntenic pairs than pear and the other three Rosaceae species. The Ka/Ks analysis of duplication PbBGLU genes in pear indicated that the main mode of expansion of the PbBGLUs was segmental replication and was mainly affected by purification. qRT-PCR showed that the three gene expression patterns of PbBGLU1, PbBGLU15 and PbBGLU16 were basically consistent with the change trend of pear fruit lignin and stone cell content, and may be involved in lignification and stone cell development of pear fruit. Subcellular localization showed that these three candidate genes were all located on the cell wall.Conclusion: In this study, a genome-wide analysis of BGLU genes in five Rosaceae species was carried out, and three candidate genes related to lignification and stone cell development of pear fruits were identified, which laid the foundation for a deeper understanding of the function of BGLU genes in pear fruits and potential in changing pear fruit quality.

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Stone cell distribution and lignin structure in various pear varieties

Cited by 81 publications

References 10 publications

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Integrated transcriptomic and proteomic analysis reveal new insights into stone cell formation in Korla pear

Genome-Wide Comparative Analysis of the β-glucosidase Family in Five Rosaceae Species and their Potential Role on Lignification of Stone Cells in Chinese White Pear

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