Stopped-flow determination of 2-mercaptopyrimidines as inductors of the iodine-azide reaction

Kurzawa, J.; Wiśniewska, Agnieszka; Janowicz, K.

doi:10.1016/j.aca.2006.03.049

Cited by 9 publications

(7 citation statements)

References 12 publications

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“…5, the optimum value of 5.5 was chosen as providing the best results. Similar results were indicated by the following parameters: volumetric titration [24][25][26], spectrophotometric [27] measurements of the course of iodine-azide reaction in an aqueous medium.…”

Section: Factors With An Impact Both On Separation and Post-column Desupporting

confidence: 81%

High-performance liquid chromatographic determination of propylthiouracil in human urine by post-column iodine–azide reaction

Zakrzewski

2008

Journal of Pharmaceutical and Biomedical Analysis

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Section: Factors With An Impact Both On Separation and Post-column Desupporting

confidence: 81%

High-performance liquid chromatographic determination of propylthiouracil in human urine by post-column iodine–azide reaction

Zakrzewski

2008

Journal of Pharmaceutical and Biomedical Analysis

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“…5). Observations conducted in the course of iodine-azide reaction measured in aqueous medium with volumetric titration (Kurzawa 1985(Kurzawa , 1987 and stoppedflow analysis (Kurzawa et al, 2006) showed much similarity.…”

Section: Chromatographic Systemmentioning

confidence: 86%

“…the highest and the fastest) consumption of iodine in the induced reaction. From the analytical point of view, this consumption can be defined as a method of measurement of the sensitivity of sulfur compound in volumetric (Kurzawa, 1985(Kurzawa, , 1987, coulometric, spectrometric and stopped-low analysis (Kurzawa et al, 2006) determination. As a result, the higher consumption of iodine the lower detection limit is observed.…”

Section: Specificity and Separationmentioning

confidence: 99%

Determination of propylthiouracil in pharmaceutical formulation by high-performance liquid-chromatography with a post-column iodine-azide reaction as a detection system

Zakrzewski

2008

Arch. Pharm. Res.

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A high-performance liquid chromatographic method with a post-column iodine-azide reaction has been chosen and tested for validity in quantitative determination of propylthiouracil in tablets. A mobile phase with a flow rate of 1.4 ml/min was conducted in the form of isocratic chromatography on a C18 column with acetonitrile-water-sodium azide solution (2.5%; pH 5.5) 24:26:50 (v/v/v). Unreacted iodine from post-column iodine-azide induced by reaction was monitored with visible detection at lambda=350 nm. The method proved both its linearity within the range of 8-100 nM (r2>0.9988) and satisfactory results of inter-day precision (RSD<4.2%) and accuracy (recovery>91%). The limits of detection (DDL) and quantification (DQL) reached the levels of 5 and 8 nM, respectively. The validation of the method comprised also its specificity. The results obtained proved the suitability and appropriateness of the suggested method for intended use.

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“…Those alternations are monitored by volumetric (Kossakowski, Kłopocki, and Ż bikowski 1972;Kurzawa 1985Kurzawa , 1987 and spectrophotometric (Kurzawa, Wiśniewska, and Janowicz 2006) analysis, slow titration with biamperometric detection of the end-point (Kurzawa 1985), and a stopped-flow technique (Kurzawa, Wiśniewska, and Janowicz 2006) in methylthiouracil analysis. Moreover, the iodineazide reaction was applied in planar chromatography for detection of the described thiol (Zakrzewski and Ciesieslki 2003).…”

Section: Introductionmentioning

confidence: 99%

Postcolumn Iodine–Azide Reaction as a Detection System in HPLC for the Determination of Methylthiouracil in Urine

Zakrzewski

2009

Analytical Letters

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This article describes a system for determining methylthiouracil in urine based on the sensitizing induction of methylthiouracil on the reaction between iodine and azide ions and combined techniques of high-performance liquid chromatography and simple postcolumn detection. The optimal conditions for iodineazide reaction and high-performance liquid chromatographic separation were determined. The reproducibility, linearity, and recovery were evaluated under the optimal conditions. The methylthiouracil standards added to normal urine show that the response of the detector, set at 350 nm (corresponding to unreacted iodine in the postcolumn iodine-azide reaction), is linear within the concentration range of 0.4-5.0 nmol mL À1 urine. The relative standard deviation values for precision and recovery within the calibration range varied from 0.9 to 4.8% and from 94 to 105%, respectively. Limits of detection (LOD) and quantitation (LOQ) were 0.3 and 0.4 nmol mL À1 urine, respectively.

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Stopped-flow determination of 2-mercaptopyrimidines as inductors of the iodine-azide reaction

Cited by 9 publications

References 12 publications

High-performance liquid chromatographic determination of propylthiouracil in human urine by post-column iodine–azide reaction

High-performance liquid chromatographic determination of propylthiouracil in human urine by post-column iodine–azide reaction

Determination of propylthiouracil in pharmaceutical formulation by high-performance liquid-chromatography with a post-column iodine-azide reaction as a detection system

Postcolumn Iodine–Azide Reaction as a Detection System in HPLC for the Determination of Methylthiouracil in Urine

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