Storage of Hydrophobic Polymers in Bacteria

Serafim, Luísa S.; Xavier, Ana M. R. B.; Lemos, Paulo C.

doi:10.1007/978-3-319-43676-0_33-1

Cited by 3 publications

(1 citation statement)

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“…PhaC genotypes appeared in a broad number of bacterial and archaeal genomes, consisting of 24.8 and 17.5%, respectively of the genome datasets, suggesting that PHAs are a relatively conserved form of carbon energy storage within bacteria ( Serafim et al, 2018 ) and archaea ( Wang et al, 2019b ). This is also supported by the distribution of Class III PhaCs in bacteria, which the study identified across 23 phyla, roughly one-fifth of the 111 recognized bacterial phyla by the Genome Taxonomy Database 8 through the standardization of bacterial taxonomy ( Parks et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Developing Bioprospecting Strategies for Bioplastics Through the Large-Scale Mining of Microbial Genomes

et al. 2021

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The accumulation of petroleum-based plastic waste has become a major issue for the environment. A sustainable and biodegradable solution can be found in Polyhydroxyalkanoates (PHAs), a microbially produced biopolymer. An analysis of the global phylogenetic and ecological distribution of potential PHA producing bacteria and archaea was carried out by mining a global genome repository for PHA synthase (PhaC), a key enzyme involved in PHA biosynthesis. Bacteria from the phylum Actinobacteria were found to contain the PhaC Class II genotype which produces medium-chain length PHAs, a physiology until now only found within a few Pseudomonas species. Further, several PhaC genotypes were discovered within Thaumarchaeota, an archaeal phylum with poly-extremophiles and the ability to efficiently use CO2 as a carbon source, a significant ecological group which have thus far been little studied for PHA production. Bacterial and archaeal PhaC genotypes were also observed in high salinity and alkalinity conditions, as well as high-temperature geothermal ecosystems. These genome mining efforts uncovered previously unknown candidate taxa for biopolymer production, as well as microbes from environmental niches with properties that could potentially improve PHA production. This in silico study provides valuable insights into unique PHA producing candidates, supporting future bioprospecting efforts toward better targeted and relevant taxa to further enhance the diversity of exploitable PHA production systems.

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Section: Discussionmentioning

confidence: 99%