Storage of pig embryos in the ligated rabbit oviduct and its effect on the viability after re-transfer to synchronized gilts

Herrmann, H.H.; Holtz, W.

doi:10.1016/0378-4320(85)90083-1

Cited by 10 publications

(7 citation statements)

References 38 publications

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“…Therefore, most or some of the 3-4-cell embryos obtained in the present study might have been derived from a similar abnormal cleavage pattern. Herrmann & Holtz (1985) found that the recovery rate and developmental rate of early pig embryos fertilized in vivo and transferred to ligated rabbit oviducts were higher for 2-cell than for 3-4-cell embryos. This indicates that 3-4-cell embryos are more sensitive to in-vitro conditions of manipulation and culture than 2-cell embryos.…”

Section: Discussionmentioning

confidence: 98%

Blastocyst formation by pig embryos resulting from in-vitro fertilization of oocytes matured in vitro

Yoshida¹,

Ishizaki²,

Kawagishi³

1990

Reproduction

107

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Section: Discussionmentioning

confidence: 98%

Blastocyst formation by pig embryos resulting from in-vitro fertilization of oocytes matured in vitro

Yoshida¹,

Ishizaki²,

Kawagishi³

1990

Reproduction

107

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“…By comparison, recovery rates for embryos originating from ovines (Lawson et al. 1972), porcines (Herrmann and Holtz 1985), bovines (Boland 1984; Sirard et al. 1985; Ellington et al.…”

Section: Discussionmentioning

confidence: 99%

“…The recovery rate of 89% accomplished with transferred latex spheres of a size corresponding to that of zona-free rabbit oocytes and mouse embryos, is probably related to the higher specific gravity (1.45 g⁄cm 3 ) that renders them less buoyant. By comparison, recovery rates for embryos originating from ovines (Lawson et al 1972), porcines (Herrmann and Holtz 1985), bovines (Boland 1984;Sirard et al 1985;Ellington et al 1990) and caprines (Rao et al 1984) that had been transferred to a ligated rabbit oviduct, ranged from 60% to almost 90%.…”

Section: Discussionmentioning

confidence: 99%

“…Thereafter, the polythene tubing was inspected under the microscope to make sure that all objects had been evacuated. The infundibulum was clamped shut for 2–3 min to prevent back‐flow, as described by Herrmann and Holtz (1985). After reposition of the genital tract, the abdominal cavity was closed with two layers of suture and the recipients were left to recover under a heat lamp.…”

Section: Methodsmentioning

confidence: 99%

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Coating of Objects Introduced into the Oviduct of Pseudopregnant Rabbit Does

El-Gayar

Holtz

2009

Reprod Domestic Animals

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This investigation addresses the possibility of providing mouse embryos or other foreign objects with a protective mucin coat by transferring them into the oviduct of a live rabbit doe. Mouse embryos at the 8 or 16-cell stage, rabbit oocytes and latex spheres resembling mouse embryos in size were transferred to the ligated oviducts of ovulation-induced rabbit does. The does were killed 24 h later to have their oviducts flushed. A large proportion of the latex spheres (89%) and of the ovulated oocytes of the recipient does (92%) was recovered. The recovery rates for transferred rabbit oocytes, either intact or with the zona pellucida removed, were 61% and 51%, respectively, whereas that for mouse embryos was extremely poor (20%). Rabbit oocytes with or without zona were enveloped in a thick mucin coat regardless whether they had been transferred or ovulated by the recipients. The same applied to empty rabbit zonae. Mouse embryos and latex spheres were also covered by a mucin coat, but it was four times thinner. While residing in the rabbit oviduct, the mouse embryos continued developing to a stage comparable to what would have been expected in situ. During the subsequent in vitro culture, mouse embryos continued developing to the expanded blastocyst stage. They did, yet, not hatch from the zona. It may be concluded that particles of various origins, when placed into the oviduct of ovulated rabbit does, will be provided with a mucin covering which is, however, considerably thinner than that surrounding oocytes or zonae pellucidae originating from rabbits.

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“…Using 4-0 DEXON "S" (Davis + Geck Inc., USA), the oviducts were ligated with one ligature on the utero-tubal junction and another on the distal end of the ampulla. Hemostasis was achieved by topical application of epinephrine dissolved in lactated Ringer's solution (0.5 mg/kg, 1:1000) and by avoiding injury to the main vessels near the oviducts according to the tubal ligation technique of Herrmann and Holtz (11). During these procedures, tissues were frequently irrigated with heparinized lactated Ringer's solution.…”

Section: Surgical Techniquementioning

confidence: 99%