Storage of platelets in balanced salt solutions: a simple platelet storage medium

Abstract

When a commercially available intravenous solution, plasmalyte-A, was added to platelet pellets as a platelet storage medium (PSM) after more than 85 percent of the plasma had been expressed, the platelets functioned equally well, after 5 days of storage, in platelet aggregation, release reaction, and serotonin uptake as did platelets stored in plasma. The level of fibrinopeptide A was significantly different in the PSM. Similarly, lower levels of kallikrein and complement components in the PSM-stored platelets indicated reduced activation of plasma enzyme systems. Morphology scores showed better shape maintenance in PSM, with values of 255 versus values of 185 in plasma (time zero, 280); electron microscopy also showed somewhat better structural maintenance in PSM. Glucose consumption, lactate and ammonia production, and fatty acid oxidation were also reduced in PSM. No significant differences in pH, white cell count, pO2, pCO2, or lactic dehydrogenase were observed after 5 days of storage in plasma or PSM; however, platelet counts were reduced after 5 days in plasma but remained constant in PSM. The beta-thromboglobulin release was significantly lower in the PSM-stored platelets after 5 days. The volume of platelet membrane glycoprotein lb (which is responsible for platelet adhesion) decreased 15 percent in plasma following storage, whereas a decrease of only 5 percent was noted in PSM. There was less leaching of plasticizers in PSM than in plasma. Survival and recovery using 51Cr-labeled autologous platelets showed that, after 5 days of storage, the recovery of PSM-stored platelets was 63 percent and the survival was 7.7 +/- 0.8 days (multiple hit model).(ABSTRACT TRUNCATED AT 250 WORDS)

Platelet glycolytic flux increases stimulated by ultraviolet‐induced stress is not the direct cause of platelet morphology and activation changes: possible implications for the role of glucose in platelet storage

“…Adams and Rock 33 presented data showing that serotonin uptake for PLTs stored in Plasmalyte A without extra glucose was only 10 percent of control PLTs stored either in the same medium with glucose or in plasma. An in vivo study done subsequent to this in vitro work demonstrated that cells stored in Plasmalyte A had 63 percent recovery and 7.7‐day survival 34 . It must be noted, however, that glucose levels on Day 5 reported in the in vivo study were 4.4‐fold higher than those found in the in vitro work (3.1 ± 0.4 vs. 0.7 ± 0.3 mmol/L), leaving a question of whether or not the PLTs in the in vivo study were truly depleted of glucose 33,34 .…”

Galactomannan Antigenemia after Infusion of Gluconate-Containing Plasma-Lyte

“…It is used for fluid resuscitation, hydration in seriously ill patients (6,11,17,20), and extended storage of platelets or peripheral blood stem cell products (3,16,19).…”

Preservation of hemostatic and structural properties of rehydrated lyophilized platelets: potential for long-term storage of dried platelets for transfusion.

“…Maintenance of critical membrane GPs during storage is crucial to platelet function in vivo. With the storage life of fresh platelets limited to 5 days, there has been considerable study to lengthen platelet shelf life and enhance stored platelet response (18)(19)(20)(21)(22)(23)(24). Investigators have addressed storage conditions that preserve platelet integrity and responsiveness (25,26).…”