Storage of washed or irradiated red cells in <scp>AS</scp>‐7 improves their <i>in vitro</i> characteristics

Proffitt, Susan; Thomas, Stephen; Swann, Ian D.; Popovsky, Mark; Smith, D. J. W.; Roberts, David J.; Cardigan, Rebecca

doi:10.1111/vox.12271

Cited by 8 publications

(8 citation statements)

References 20 publications

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“…Previous studies have shown that human RBCs stored with SOLX maintain ATP and 2,3 DPG, and lower increase in lactate and extracellular K + compared to other preservation solutions [20, 32]. Our results confirm that rat RBCs closely mimics human RBC changes.…”

Section: Discussionsupporting

confidence: 86%

Rat red blood cell storage lesions in various additive solutions

Jani

Yalçın

Williams

et al. 2017

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Peer reviewed eScholarship.orgPowered by the California Digital Library University of California U n c o r r e c t e d A u t h o r P r o o fClinical Hemorheology and Microcirculation xx (20xx) RESULTS:There were no differences in biochemical or mechanical parameters at baseline or after one week of storage. 18However, after two weeks, AS-7 preserved biochemical and mechanical properties as compared to CPDA-1 and AS-1. 19Changes were observed to be significant after 14-days of storage. AS-7 prevented extracellular K + increase, reduced acidosis, 20showed lower hemolysis, preserved ATP and 2,3-DPG levels (consequently oxygen affinity), and reduced lactate. AS-7, when 21 compared to CPDA-1 and AS-1, prevented the reduction in RBC deformability and was found to preserve the EI at multiple 22 shear stresses, the membrane SEM, the aggregability and viscosity.

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Section: Discussionsupporting

confidence: 86%

Rat red blood cell storage lesions in various additive solutions

Jani

Yalçın

Williams

et al. 2017

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“…It has been reported that some changes occur during the course of storage [ 4 ]. During storage, progressive morphological and biochemical changes occur which are often related to the reduction of ATP, 2,3-biphosphoglycerate, and NADH in red blood cells (RBCs) [ 5 , 6 ]. These changes are referred to as the “storage lesions” [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…During storage, progressive morphological and biochemical changes occur which are often related to the reduction of ATP, 2,3-biphosphoglycerate, and NADH in red blood cells (RBCs) [ 5 , 6 ]. These changes are referred to as the “storage lesions” [ 5 ]. There is also strong evidence that oxidative stress plays a major role in the storage lesions [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

The Relationship of Oxidation Sensitivity of Red Blood Cells and Carbonic Anhydrase Activity in Stored Human Blood: Effect of Certain Phenolic Compounds

Huyut

Şekeroğlu

Balahoroğlu

et al. 2016

BioMed Research International

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It has been reported that many modifications occur with the increase of oxidative stress during storage in erythrocytes. In order to delay these negative changes, we evaluated whether the addition of substances likely to protect antioxidant capacity in stored blood would be useful. Therefore, we investigated the effects of resveratrol, tannic acid, and caffeic acid in lipid peroxidation and antioxidant capacity of erythrocytes in stored blood. Donated blood was taken into four CPD containing blood bags. One bag was used as the control, and the others were supplemented with caffeic acid (30 μg/mL), resveratrol (30 μg/mL), and tannic acid (15 μg/mL), respectively. Erythrocyte lipid peroxidation, sensitivity to oxidation, glutathione levels and carbonic anhydrase, glutathione peroxidase, and catalase activities were measured on days 0, 7, 14, 21, and 28. In the control group, erythrocyte malondialdehyde levels and sensitivity to oxidation were increased whereas glutathione, glutathione peroxidase, and catalase levels were decreased (p < 0.05). Resveratrol and caffeic acid prevented malondialdehyde accumulation and preserved glutathione, glutathione peroxidase, and catalase activities in erythrocytes. We demonstrated that resveratrol, caffeic acid, and tannic acid in stored blood could decrease the sensitivity to oxidation of erythrocytes in vitro but did not exhibit such effects on CA activity.

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“…Several alkaline, chloride‐free and hypotonic solutions were researched into, such as ErythroSol, ErythroSol‐1 and EAS‐81 (also known as SOLX and AS‐7 currently) . One promising AS utilizing the chloride shift is AS‐7 . AS‐7 contains phosphate and bicarbonate at an alkaline pH of 8·4, which improves the buffering capacity of the AS leading to higher glucose consumption and ATP generation with better and longer RBC storage.…”

Section: Red Cell Additive Solutionsmentioning

confidence: 99%

“…In vivo recovery of RBCs stored in AS‐7 was 88 ± 5% at 42 days and 82 ± 3% at 56 days, outperforming the first generation ASs . RBC haemolysis and 24‐h recovery at Day 42 is well maintained after overnight hold of whole blood at 20–24°C and after RBC washing and irradiation . US FDA cleared AS‐7 for 42‐day RBC storage and CE mark approved for 56 days.…”

Section: Red Cell Additive Solutionsmentioning

confidence: 99%

Advances in blood storage bags and preservative solutions

Tsoi¹

2016

ISBT Science Series

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The design of storage systems for blood component focuses on preservation of the physical integrity of the blood components and their functions as well as maintaining their safety profile for clinical transfusion. The systems must be easily accessible and ready for use in hospitals, and they are affordable to those in need. There is consensus in the healthcare community that the use of di‐ethylhexyl phthalate (DEHP), a plasticizer commonly used in blood storage bags, should be minimized in light of the potential risks of endocrine disruption in high‐risk populations such as neonates, pregnant and nursing women. Candidate DEHP replacements in blood container systems have then become the objectives of research studies and commercial activities. Moreover, there were advancements in technology of the development of blood cell preservative solutions leading to improvement in the quality of blood component upon storage and logistic advantages of blood component preparation in the past decades.

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Storage of washed or irradiated red cells in AS‐7 improves their in vitro characteristics

Abstract: These data suggest that the quality of these components may be improved by storage in AS-7 compared with SAGM.

Cited by 8 publications

References 20 publications

Rat red blood cell storage lesions in various additive solutions

Rat red blood cell storage lesions in various additive solutions

The Relationship of Oxidation Sensitivity of Red Blood Cells and Carbonic Anhydrase Activity in Stored Human Blood: Effect of Certain Phenolic Compounds

Advances in blood storage bags and preservative solutions

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