The Relationship of Oxidation Sensitivity of Red Blood Cells and Carbonic Anhydrase Activity in Stored Human Blood: Effect of Certain Phenolic Compounds

Abstract

It has been reported that many modifications occur with the increase of oxidative stress during storage in erythrocytes. In order to delay these negative changes, we evaluated whether the addition of substances likely to protect antioxidant capacity in stored blood would be useful. Therefore, we investigated the effects of resveratrol, tannic acid, and caffeic acid in lipid peroxidation and antioxidant capacity of erythrocytes in stored blood. Donated blood was taken into four CPD containing blood bags. One bag was used as the control, and the others were supplemented with caffeic acid (30 μg/mL), resveratrol (30 μg/mL), and tannic acid (15 μg/mL), respectively. Erythrocyte lipid peroxidation, sensitivity to oxidation, glutathione levels and carbonic anhydrase, glutathione peroxidase, and catalase activities were measured on days 0, 7, 14, 21, and 28. In the control group, erythrocyte malondialdehyde levels and sensitivity to oxidation were increased whereas glutathione, glutathione peroxidase, and catalase levels were decreased (p < 0.05). Resveratrol and caffeic acid prevented malondialdehyde accumulation and preserved glutathione, glutathione peroxidase, and catalase activities in erythrocytes. We demonstrated that resveratrol, caffeic acid, and tannic acid in stored blood could decrease the sensitivity to oxidation of erythrocytes in vitro but did not exhibit such effects on CA activity.

Actions of resveratrol and serotonin added to stored bloods on lipid peroxidation and oxidative DNA damage

“…Because of a possible increase in 8-OHDG levels in stored blood can cause gene mutations and false protein coding after blood transfusion, it is important to prevent injury in the DNA bases. Increasing oxidative stress and decreasing antioxidant capacity due to storage time (3,9,15,17,26), especially OH . radicals, can lead to DNA oxidation by damaging nuclear proteins and DNA (9).…”

“…A total of 60 µg/mL resveratrol and 60 µg/mL serotonin was added to the second and third group, respectively. For blood storage time and measurement intervals, time intervals specified by previous studies were selected (15,17,26). For this purpose, MDA and oxidized CoQ10 were measured in the samples taken at the beginning, 7, 14, 21 and 28 days in all groups.…”

“…This can lead to budget, labor and time losses. Some studies have suggested that the loss of the antioxidant defense system and increased oxidative stress during and after storage shorten the shelf life time of blood cells (15,16). For this reason, it has been shown that adding some additives such as antioxidants to blood bags has a positive effect in order to increase the shelf life by protecting the antioxidant capacities of stored blood (8,17).…”

The susceptibility to autoxidation of erythrocytes in diabetic mice: Effects of melatonin and pentoxifylline

“…The susceptibility to in vitro oxidation of erythrocytes was performed according to the method used by Huyut et al . For this purpose, a suspension of erythrocyte with azide buffer was prepared and MDA level was measured spectrophotometrically at 532 nm with thiobarbituric acid (TBA) reagent (baseline).…”

In Stored Human Blood, The Inhibitor Effect of Tannic Acid and Caffeic Acid on Lipid Peroxidation and Oxidative DNA Damage

“…These criterias; during the storage period rate of hemolysis in erythrocytes should not exceed 1% and after the transfusion at least 75% of RBCs should stay alive in the first 24 hours (3). In many clinical situation, blood transfusion is necessary, such as deep anemia, acute blood loss and some surgeries (4,5). Several studies indicated that while the stored blood maintained at optimum conditions, some adverse change occurs called as "storage lesion" time-dependently.…”