Storage oil hydrolysis during early seedling growth

Quettier, Anne-Laure; Eastmond, Peter J.

doi:10.1016/j.plaphy.2008.12.005

Cited by 137 publications

(119 citation statements)

References 53 publications

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…Storage oil breakdown takes place immediately after seed germination and provides the C skeletons and energy that support seeding growth following germination (Quettier and Eastmond, 2009). Most of the oil bodies in Arabidopsis seedlings disappeared within 6 d after germination on 60 mM N (Siloto et al, 2006).…”

Section: There Is a Homeostasis Between Tag Biosynthesis And Degradatmentioning

confidence: 99%

ABI4 Activates DGAT1 Expression in Arabidopsis Seedlings during Nitrogen Deficiency

et al. 2011

View full text Add to dashboard Cite

Triacylglycerol (TAG) is the major seed storage lipid and is important for biofuel and other renewable chemical uses. Acylcoenzyme A:diacylglycerol acyltransferase1 (DGAT1) is the rate-limiting enzyme in the TAG biosynthesis pathway, but the mechanism of its regulation is unknown. Here, we show that TAG accumulation in Arabidopsis (Arabidopsis thaliana) seedlings increased significantly during nitrogen deprivation (0.1 mM nitrogen) with concomitant induction of genes involved in TAG biosynthesis and accumulation, such as DGAT1 and OLEOSIN1. Nitrogen-deficient seedlings were used to determine the key factors contributing to ectopic TAG accumulation in vegetative tissues. Under low-nitrogen conditions, the phytohormone abscisic acid plays a crucial role in promoting TAG accumulation in Arabidopsis seedlings. Yeast one-hybrid and electrophoretic mobility shift assays demonstrated that ABSCISIC ACID INSENSITIVE4 (ABI4), an important transcriptional factor in the abscisic acid signaling pathway, bound directly to the CE1-like elements (CACCG) present in DGAT1 promoters. Genetic studies also revealed that TAG accumulation and DGAT1 expression were reduced in the abi4 mutant. Taken together, our results indicate that abscisic acid signaling is part of the regulatory machinery governing TAG ectopic accumulation and that ABI4 is essential for the activation of DGAT1 in Arabidopsis seedlings during nitrogen deficiency.

show abstract

Section: There Is a Homeostasis Between Tag Biosynthesis And Degradatmentioning

confidence: 99%

ABI4 Activates DGAT1 Expression in Arabidopsis Seedlings during Nitrogen Deficiency

et al. 2011

View full text Add to dashboard Cite

show abstract

“…In germinated oilseeds, mobilization of the stored fatty acid is essential to supply energy and carbon for embryonic growth. Lipolytic enzymes catalyze the first step of lipid mobilization, with the possibility of subsequently being controlled during and after the germination period (Quettier and Eastmond, 2009;Borgston e Brockman, 1984).…”

Section: Seed Lipasesmentioning

confidence: 99%

“…Table 1 shows that the seed lipases evaluated had greater activity for the triacylglycerols containing the fatty acids found in higher percentages in the nutrient reserve tissue of that seed. Such affinity is of great physiological significance for the seed (Quettier and Eastmond, 2009;Hellyer et al, 1999;Hammer and Murphy, 1993).…”

Section: Specificity Of Seed Lipasesmentioning

confidence: 99%

Seed lipases: sources, applications and properties - a review

Barros

Fleuri

Macêdo

2010

Braz. J. Chem. Eng.

178

100

View full text Add to dashboard Cite

-This paper provides an overview regarding the main aspects of seed lipases, such as the reactions catalyzed, physiological functions, specificities, sources and applications. Lipases are ubiquitous in nature and are produced by several plants, animals and microorganisms. These enzymes exhibit several very interesting features, such as low cost and easy purification, which make their commercial exploitation as industrial enzymes a potentially attractive alternative. The applications of lipases in food, detergents, oils and fats, medicines and fine chemistry, effluent treatment, biodiesel production and in the cellulose pulp industry, as well as the main sources of oilseed and cereal seed lipases, are reviewed.

show abstract

“…Among them is the mobilization of triacylglycerol (TAG) in oilseeds, which occurs following germination and supports early seedling growth, until photosynthetic competence is achieved [9]. Within the oral and poster sessions on lipid catabolism new finding were presented concerning the mechanism of TAG hydrolysis, fatty acid transport, activation and β-oxidation, which are all required for normal seedling establishment in Arabidopsis.…”

Section: Lipid Catabolism Sessionmentioning

confidence: 99%