Storage Period of Liquid Semen Eligible for Insemination in Thin Tail Sheep Semen Diluted with Egg Yolk Citrate with the Addition of Noni (Morinda citrifolia Linn) Fruit Extract

Abstract

Semen processing can reduce the motility and viability of the sperm. The focus of this research was to evaluate the shelflife of liquid semen which could still be used for insemination in thin tail sheep semen diluted with Egg Yolk Citrate and noni (Morinda citrifolia Linn) extract. Semen was collected from 2 years-old thin tail sheep using an artificial vagina. The sperm was analyzed at the UGM Faculty of Animal Science's Laboratory of Animal Physiology and Reproduction. In this research, the treatments were egg yolk citrate diluent (P0) as a control, P0 + 4% noni extract (P1), P0 + 6% noni extract (P2), and P0 + 8% noni extract (P3). The variables measured were the macroscopic quality of the semen as well as microscopic quality of spermatozoa. The results showed that the addition of noni extract could improve (P<0.05) motility and viability of spermatozoa stored at 5 o C. The addition of 4% noni fruit extract generated the best results in terms of preserving motility (44.2%) and spermatozoa viability (68.4%) after 5 days of storage. The conclusion of this study, day five storage of thin tail sheep liquid semen diluted with egg yolk citrate and 4% noni fruit extract at 5 o C was still eligible for insemination.

Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram

“…The ROS produced by spermatozoa and leukocytes that infiltrate the semen in the ejaculate are responsible for the dysfunction of mammalian semen. Lipid peroxidation, unsustainable spermatozoa motility and cell nucleus malfunction are the three mechanisms by which free radicals contribute to cell death (49). Oxidative damage is more likely to occur in cryopreserved semen than in fresh ejaculate.…”