In this chapter, we review the level of disturbance caused by railways due to noise and vibration, air, soil and water pollution, and soil erosion. There is evidence that soil and hydrology contamination may affect vegetation and aquatic fauna while noise can affect terrestrial vertebrates. In fact, noise, light, and vibration due to railways have been observed to reduce the abundance and richness of some insects, amphibians, and birds, and to cause avoidance behaviour on predators. Interestingly, reptiles, some bird species, small mammals, and large mammals seem to ignore rail traffic and benefit from the vegetation planted in the railway verges that provide food and shelter. Some engineering structures have been implemented to reduce the effects of railway disturbance: rail fastenings, rail dampers, under-sleeper pads, and noise barriers are applied to minimize noise and vibration; washing with water and cleaning the ballast are used to mitigate soil pollution; and grass plantation, the use of gypsum and application of compost/mulch coverage, are applied to control soil erosion.