2010
DOI: 10.1021/bi100448f
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STPR, a 23-Amino Acid Tandem Repeat Domain, Found in the Human Function-Unknown Protein ZNF821

Abstract: The STPR motif is composed of 23-amino acid repeats aligned contiguously. STPR was originally reported as the DNA-binding domain of the silkworm protein FMBP-1. ZNF821, the human protein that contains the STPR domain, is a zinc finger protein of unknown function. In this study, we prepared peptides of silkworm FMBP-1 STPR (sSTPR) and human ZNF821 STPR (hSTPR) and compared their DNA binding behaviors. This revealed that hSTPR, like sSTPR, is a double-stranded DNA-binding domain. Sequence-independent DNA binding… Show more

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Cited by 7 publications
(6 citation statements)
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“…DNA-binding domains, such as the helix-turn-helix, zinc finger, and leucine zipper domains, are well-known and serve to structurally classify DNA-binding domains (56). However, this study and the excellent descriptions by others demonstrate that TR DNA-binding domains occur in prokaryotes and eukaryotes and represent a new class of DNA-binding proteins (9,46,52).…”
Section: Discussionmentioning
confidence: 80%
“…DNA-binding domains, such as the helix-turn-helix, zinc finger, and leucine zipper domains, are well-known and serve to structurally classify DNA-binding domains (56). However, this study and the excellent descriptions by others demonstrate that TR DNA-binding domains occur in prokaryotes and eukaryotes and represent a new class of DNA-binding proteins (9,46,52).…”
Section: Discussionmentioning
confidence: 80%
“…In addition, this class contains 3 genes ( ZNF592 , 653 and 821 ) encoding zinc finger proteins that are not well known. The affiliation of these genes with the zinc finger family suggested that they function in the regulation of the transcription, as observed for ZNF821 , which contains a domain reported to be a novel DNA-binding motif [29]. Moreover, ZNF653 has recently been characterized as a novel protein affecting transcription through the mediation of several nuclear receptors, such as peroxisomal proliferator-activated receptor gamma, which is known to mediate the expression of lipogenic genes and to be negatively correlated with the concentration of trans -FA and CLA isomers in milk [30].…”
Section: Discussionmentioning
confidence: 99%
“…). Previous findings on human ZNF821, which contains a STPR motif in the C‐terminal tail, also indicated that STPR contributes to nuclear localization . Thus, the nuclear localization function of the STPR motif might be widely conserved among STPR‐containing proteins.…”
Section: Discussionmentioning
confidence: 85%
“…The sequential illumination periods during the measurement of fluorescence intensity were 15.0 μs each. The data were processed in essentially the same manner as described previously , except that the measurement time (60 s, consisting of six consecutive measurements of 10 s each) differed. For quantitative analyses, the fluorescence ACF G( τ ) was calculated as follows: Gnormalτ=ItIt+τIfalse(tfalse)2,where τ represents the time delay, I is the fluorescence intensity, and the brackets denote the ensemble average.…”
Section: Methodsmentioning
confidence: 99%
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