STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination

Dirks, Wilhelm G.; Drexler, Hans

doi:10.1007/978-1-62703-128-8_3

Cited by 57 publications

(54 citation statements)

References 9 publications

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“…Cells placed under selective pressure (drug resistance, antibiotic selection) can frequently select for a previously undetectable, underlying contaminant population; thus, frequent DNA profiling is advised. Non-human (inter-species) cell line contamination is less well publicized but thought to affect 6 percent of cultures (24). Moreover, current STR and SNP profiling does not detect this form of contamination.…”

Section: Non-human Cell Linessupporting

confidence: 42%

Cell line authentication to improve preclinical cancer research: Methods in cell line authentication, quality control, and annotation

Ethier¹,

Neve²

2015

AACR Education book

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Section: Non-human Cell Linessupporting

confidence: 42%

Cell line authentication to improve preclinical cancer research: Methods in cell line authentication, quality control, and annotation

Ethier¹,

Neve²

2015

AACR Education book

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“…[21][22][23][24] Recent techniques using DNA fingerprinting of short tandem repeats (STRs) at selected loci within the genome offer a simple, inexpensive, and rapid method of authenticating cell lines. [25][26][27] We have recently conducted an STR analysis of the endometrial cell line HES, developed in our laboratory in 1989. 9 We discovered that the HES cell line exhibited a DNA fingerprint indistinguishable from that of the HeLa cell DNA fingerprint.…”

Section: Introductionsupporting

confidence: 43%

Discovery of HeLa Cell Contamination in HES Cells: Call for Cell Line Authentication in Reproductive Biology Research

Kniss

Summerfield

2014

Reprod. Sci.

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Continuous cell lines are used frequently in reproductive biology research to study problems in early pregnancy events and parturition. It has been recognized for 50 years that many mammalian cell lines contain inter-or intraspecies contaminations with other cells. However, most investigators do not routinely test their culture systems for cross-contamination. The most frequent contributor to cross-contamination of cell lines is the HeLa cell isolated from an aggressive cervical adenocarcinoma. We report on the discovery of HeLa cell contamination of the human endometrial epithelial cell line HES isolated in our laboratory. Short tandem repeat analysis of 9 unique genetic loci demonstrated molecular identity between HES and HeLa cells. In addition, we verified that WISH cells, isolated originally from human amnion epithelium, were also contaminated with HeLa cells. Inasmuch as our laboratory did not culture HeLa cells at the time of HES cell derivations, the source of contamination was the WISH cell line. These data highlight the need for continued diligence in authenticating cell lines used in reproductive biology research.

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“…HeLa)(Dirks and Drexler 2013; Dolgin 2016; Gartler 1968; Hughes et al 2007; Landry et al 2013; Pamies et al 2017; Reid 2017). During the last decades, as cross-contaminations and cellular misidentifications became apparent, the importance of cell line identity gained increasing attention (Dirks and Drexler 2013; Freedman 2015; Freedman et al 2015b). The rapid development in the field of genome sequencing allowed us now to address questions on genetic drift and stability at higher levels of resolution.…”

Section: Discussionmentioning

confidence: 99%

Major changes of cell function and toxicant sensitivity in cultured cells undergoing mild, quasi-natural genetic drift

et al. 2018

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Genomic drift affects the functional properties of cell lines, and the reproducibility of data from in vitro studies. While chromosomal aberrations and mutations in single pivotal genes are well explored, little is known about effects of minor, possibly pleiotropic, genome changes. We addressed this question for the human dopaminergic neuronal precursor cell line LUHMES by comparing two subpopulations (SP) maintained either at the American-Type-Culture-Collection (ATCC) or by the original provider (UKN). Drastic differences in susceptibility towards the specific dopaminergic toxicant 1-methyl-4-phenylpyridinium (MPP+) were observed. Whole-genome sequencing was performed to identify underlying genetic differences. While both SP had normal chromosome structures, they displayed about 70 differences on the level of amino acid changing events. Some of these differences were confirmed biochemically, but none offered a direct explanation for the altered toxicant sensitivity pattern. As second approach, markers known to be relevant for the intended use of the cells were specifically tested. The “ATCC” cells rapidly down-regulated the dopamine-transporter and tyrosine-hydroxylase after differentiation, while “UKN” cells maintained functional levels. As the respective genes were not altered themselves, we conclude that polygenic complex upstream changes can have drastic effects on biochemical features and toxicological responses of relatively similar SP of cells.Electronic supplementary materialThe online version of this article (10.1007/s00204-018-2326-5) contains supplementary material, which is available to authorized users.

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STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination

Cited by 57 publications

References 9 publications

Cell line authentication to improve preclinical cancer research: Methods in cell line authentication, quality control, and annotation

Cell line authentication to improve preclinical cancer research: Methods in cell line authentication, quality control, and annotation

Discovery of HeLa Cell Contamination in HES Cells: Call for Cell Line Authentication in Reproductive Biology Research

Major changes of cell function and toxicant sensitivity in cultured cells undergoing mild, quasi-natural genetic drift

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