STR typing of ancient DNA extracted from hair shafts of Siberian mummies

Amory, Sylvain; Keyser, Christine; Crubézy, Éric; Ludes, B.

doi:10.1016/j.forsciint.2006.05.042

Cited by 43 publications

(19 citation statements)

References 22 publications

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“…Then, the bones were placed in a microwave oven (5 x 1 min) to eliminate soft tissues [12]. The marrow remaining in the bone matrix was cleaned with distilled water and the bone was then crushed with a extremely cold, dry climate of this region generally provided a well-preserved nucleic acid chain [13]. Of the six specimens studied, two were identified as newborns using the method described by Scheuer and Black [14].…”

Section: Samplesmentioning

confidence: 99%

“…The ancient bones were treated with extreme precautions during each step of sample preparation. DNA extraction and PCR amplification were carried out in a DNA laboratory specifically for ancient bones as described elsewhere [13,17]. In both laboratories, the technical personnel handling bone material were genotyped for the neighbouring 189 mtDNA region.…”

Section: Samplesmentioning

confidence: 99%

See 1 more Smart Citation

Detection of the A189G mtDNA heteroplasmic mutation in relation to age in modern and ancient bones

Lacan

Thèves

Amory³

et al. 2008

Int J Legal Med

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Abstract:The aim of this study was to demonstrate the presence of the A189G age-related point mutation on DNA extracted from bone. For this, a PNA/DNA sequencing method which can determine an age threshold for the appearance of the mutation was used.Initially, work was done in muscle tissue in order to evaluate the sensitivity of the technique and afterwards in bone samples from the same individuals. This method was also applied to ancient bones from 6 well preserved skeletal remains. The mutation was invariably found in muscle, and at a rate of up to 20% in individuals over 60 years old.In modern bones, the mutation was detected in individuals aged 38 years old or more, at a rate of up to 1%, but its occurrence was not systematic (only 4 out of 10 of the individuals over 50 years old carried the heteroplasmy). For ancient bones, the mutation was also found in the oldest individuals according to osteologic markers. The study of this type of age-related mutation and a more complete understanding of its manifestation has potentially useful applications. Combined with traditional age markers, it could improve identification accuracy in forensic cases or in anthropological studies of ancient populations.

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Section: Samplesmentioning

confidence: 99%

Section: Samplesmentioning

confidence: 99%

Detection of the A189G mtDNA heteroplasmic mutation in relation to age in modern and ancient bones

Lacan

Thèves

Amory³

et al. 2008

Int J Legal Med

View full text Add to dashboard Cite

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“…Nevertheless, recently, there have been a growing number of aDNA studies that report the successful recovery of nuDNA [3][4][5][6][7]. Almost all of these studies performed on human remains have focused on the amelogenin gene for sex identification and on short tandem repeat loci (STRs) using commercially available typing kits developed for forensic applications [8][9][10][11][12]. Autosomal STR typing generally enables individual identification and deciphering close kinship relationship, while Y-chromosomal STRs are useful to follow-up paternal lineages analogous to the conventional sequencing of mtDNA hypervariable region I (HV-I) that allows the following of maternal lineages.…”

Section: Introductionmentioning

confidence: 99%

Pigment phenotype and biogeographical ancestry from ancient skeletal remains: inferences from multiplexed autosomal SNP analysis

Bouakaze¹,

Keyser

Crubézy

et al. 2009

Int J Legal Med

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In the present study, a multiplexed genotyping assay for ten single nucleotide polymorphisms (SNPs) located within six pigmentation candidate genes was developed on modern biological samples and applied to DNA retrieved from 25 archeological human remains from southern central Siberia dating from the Bronze and Iron Ages. SNP genotyping was successful for the majority of ancient samples and revealed that most probably had typical European pigment features, i.e., blue or green eye color, light hair color and skin type, and were likely of European individual ancestry. To our knowledge, this study reports for the first time the multiplexed typing of autosomal SNPs on aged and degraded DNA. By providing valuable information on pigment traits of an individual and allowing individual biogeographical ancestry estimation, autosomal SNP typing can improve ancient DNA studies and aid human identification in some forensic casework situations when used to complement conventional molecular markers.

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“…Once the aforementioned investigations are completed, prior to digestion of cellular components, each hair must be cleaned to remove any extraneous sources of DNA that may not be derived from the hair donor [12,13]. This washing process, though essential for removal of exterior contaminants, can potentially remove epithelial cells adhering to the shaft surface, and so should be carried out with caution [14]. DNA must then be separated from all other biological components in order for analysis to be undertaken.…”

Section: Dna Recoverymentioning

confidence: 99%

DNA reviews: hair

Graham

2007

Forens Sci Med Pathol

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Human and nonhuman hairs are often recovered during forensic investigation. As with all other biological samples that may be collected, via DNA analysis, hairs have the potential to provide the investigating authority with valuable intelligence pertaining to the identity of offenders, victims and even pets. DNA analysis of hairs is not however a straightforward process. The biochemical make up of hairs provide the DNA analyst with a unique set of challenges that must be overcome before any useful information can be gleaned from the evidence. This short review provides an overview of the location and condition of DNA within hair samples, and discusses the analytical methods that are employed to maximise the information that can be obtained from this sample type.

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STR typing of ancient DNA extracted from hair shafts of Siberian mummies

Cited by 43 publications

References 22 publications

Detection of the A189G mtDNA heteroplasmic mutation in relation to age in modern and ancient bones

Detection of the A189G mtDNA heteroplasmic mutation in relation to age in modern and ancient bones

Pigment phenotype and biogeographical ancestry from ancient skeletal remains: inferences from multiplexed autosomal SNP analysis

DNA reviews: hair

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