Straightforward Synthesis of L‐PEI‐Coated Gold Nanoparticles and Their Biological Evaluation

Bouché, Mathilde; Fournel, Sylvie; Kichler, Antoine; Selvam, Tamilselvi; Gallani, Jean‐Louis; Bellemin‐Laponnaz, Stéphane

doi:10.1002/ejic.201800489

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…Another study has a similar result, where 10 nm diameter PEI coated AuNP showed to be biocompatible through in vitro cytotoxicity studies, where in gold concentrations of up to 400 μM they were shown to be non-toxic to three different cancer cell lines (HCT116 colorectal carcinoma, MCF7 breast adenocarcinoma and PC3 prostate adenocarcinoma) [121]. Whereas, the 23 nm diameter PEI coated AuNP showed a moderate level of cytotoxicity to the same three cancer cell lines, with IC 50 results below 80 μM [121]. In both experiments the larger nanoparticles proved to be more cytotoxic then their smaller counterparts.…”

Section: Toxicitymentioning

confidence: 84%

“…Interestingly, when 40 nm PAA coated AuNP were used, cell viability dropped considerably to only 10% using the same gold concentration [120]. Another study has a similar result, where 10 nm diameter PEI coated AuNP showed to be biocompatible through in vitro cytotoxicity studies, where in gold concentrations of up to 400 μM they were shown to be non-toxic to three different cancer cell lines (HCT116 colorectal carcinoma, MCF7 breast adenocarcinoma and PC3 prostate adenocarcinoma) [121]. Whereas, the 23 nm diameter PEI coated AuNP showed a moderate level of cytotoxicity to the same three cancer cell lines, with IC 50 results below 80 μM [121].…”

Section: Toxicitymentioning

confidence: 92%

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Biomedical applications of polyelectrolyte coated spherical gold nanoparticles

Fuller

Köper

2019

Nano Convergence

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Surface modified gold nanoparticles are becoming more and more popular for use in biomaterials due to the possibility for specific targeting and increased biocompatibility. This review provides a summary of the recent literature surrounding polyelectrolyte coatings on spherical gold nanoparticles and their potential biomedical applications. The synthesis and layer-by layer coating approach are briefly discussed together with common characterisation methods. The potential applications and recent developments in drug delivery, gene therapy, photothermal therapy and imaging are summarized as well as the effects on cellular uptake and toxicity. Finally, the future outlook for polyelectrolyte coated gold nanoparticles is explored, focusing on their use in biomedicine.

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Section: Toxicitymentioning

confidence: 84%

Section: Toxicitymentioning

confidence: 92%

Biomedical applications of polyelectrolyte coated spherical gold nanoparticles

Fuller

Köper

2019

Nano Convergence

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“…Assemblies of this charge are observably taken up and internalized more easily by the negatively charged cell membrane compared to particles with negative and neutral surface charge [69,70]. Overall positive particle charge promotes high electrostatic affinity for biological molecules, and increases efficiency of uptake [71]. Particles with very high positive charge though can potentially be taken up indiscriminately increasing offtarget effects [72].…”

Section: Pyridine-2-thione Assaymentioning

confidence: 99%

Active targeting of CD4⁺ T lymphocytes by PEI-capped, peptide-functionalized gold nanoparticles

Ncobeni¹,

laTorre²,

Alberício³

et al. 2022

Nanotechnology

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Active targeting, which comprises recognition and attachment to target cells through receptor-ligand binding, is a promising approach for the treatment of viral infections, both as a supplement and as a potential replacement to conventional system-wide therapy. In particular, site-specific formulations for the treatment of HIV infection may overcome challenges associated with current ARV regimens. These challenges include toxicity, drug resistance and the existence of viral reservoirs. In this study we explored active targeting by synthesizing a gold nanoparticle construct decorated with an anti-CD4 cyclic peptide. The aim was to demonstrate selectivity of the system for the CD4 receptor and to deliver the RNA payload into T-lymphocytes. Colloidal gold nanoparticles functionalized with N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) were formed by a one-pot synthesis method where thiol modified polyethyleneimine (PEI) was mixed with chloroauric acid. PEI-SPDP AuNPs (gold nanoparticles) were conjugated to an anti-CD4 peptide and loaded with RNA. We measured toxicity and uptake using TZM-bl and HeLa cells. Our findings show that the nanoparticles bind selectively to CD4+ cells. No internal RNA delivery was demonstrated. Further work is required to improve internalization.

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“…In other case, the reducing substance can play the role of a reducing agent and a ligand, which forms a complex compound with the available metal ion. It is typical for reducing polymers including a nitrogen atom as heteroatom, e.g., poly(vinylpyrrolidone) (PVP), polyethylenimine (PEI), or polyaniline (PANI) [168][169][170][171][172][173][174]. Such a formation of a complex compound is widely applied in practice for the tailored preparation of metal nanoparticles.…”

Section: Preparation Of Metal Nanoparticles By Chemical Reduction: Romentioning

confidence: 99%

Physicochemical Aspects of Metal Nanoparticle Preparation

Kvı́tek¹,

Prucek²,

Panáček³

et al. 2020

Engineered Nanomaterials - Health and Safety

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Physicochemical properties, including optical properties or catalytic activity, and biological properties of metal nanoparticles are considerably influenced by their diameter. Therefore, a tailored synthesis of metal nanoparticles represents a key topic in the field of nanotechnology, and the number of research papers, concerning this topic, has been annually growing with an arithmetic progression. Metal nanoparticles are most frequently prepared via chemical reduction of metals in ionic form from their solutions. Using this synthetic approach, tailored parameters of the particles can be achieved via the adjustment of numerous factors: difference of potentials of the metal redox system and the reducing agent redox system, pH of the reaction mixture, and its temperature. The influence of these three factors on the diameter of the prepared metal nanoparticles will be discussed in the following chapter with respect to general laws and based on numerous examples from research practice.

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Straightforward Synthesis of L‐PEI‐Coated Gold Nanoparticles and Their Biological Evaluation

Cited by 6 publications

References 21 publications

Biomedical applications of polyelectrolyte coated spherical gold nanoparticles

Biomedical applications of polyelectrolyte coated spherical gold nanoparticles

Active targeting of CD4⁺ T lymphocytes by PEI-capped, peptide-functionalized gold nanoparticles

Physicochemical Aspects of Metal Nanoparticle Preparation

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Straightforward Synthesis of L‐PEI‐Coated Gold Nanoparticles and Their Biological Evaluation

Cited by 6 publications

References 21 publications

Biomedical applications of polyelectrolyte coated spherical gold nanoparticles

Biomedical applications of polyelectrolyte coated spherical gold nanoparticles

Active targeting of CD4+ T lymphocytes by PEI-capped, peptide-functionalized gold nanoparticles

Physicochemical Aspects of Metal Nanoparticle Preparation

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Product

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Active targeting of CD4⁺ T lymphocytes by PEI-capped, peptide-functionalized gold nanoparticles