Strain Differences in the Immunogenicity of Aggregated Human IgG and the Adjuvant Action of Lipopolysaccharide on the Low‐Responder Strain of Mice

Abstract

Strain differences in the antibody response to human IgG (HGG) were observed when aggregated HGG was injected intravenously. Lipopolysaccharide (LPS) administered subsequently markedly enhanced the antibody response to HGG in low responder C57BL/6 mice as compared with that in high responder DDD, C3H/He or (C57BL/6 × DDD)F1 mice. Aggregate‐free preparation of HGG at a dose of 0.5 mg induced immunological tolerance in all strains of mice tested. LPS injected subsequently converted tolerogenic, aggregate‐free HGG into immunogen in DDD mice but not in C57BL/6 mice. To determine the correlation between adjuvanticity and mitogenicity of LPS, spleen cells from normal mice were cultured in the presence of LPS and 3H‐thymidine uptake was measured. Spleen cells of DDD mice incorporated three times as much 3H‐thymidine as those of C57BL/6 mice. There seems no strong correlation between both activities of LPS. The data obtained are discussed in terms of strain differences in the macrophage function for processing the antigen.

Immunogenicity of different stressed IgG monoclonal antibody formulations in immune tolerant transgenic mice

“…In fact, several studies have shown that the administration of aggregate-free foreign IgG can be very well tolerated in different animal species and in human patients, sometimes even when formulated in incomplete Freund's adjuvant or with lipopolysaccharides as adjuvants. [40][41][42][43][44] fragments, meaning that not all the disulfide bridges were broken. Interestingly, the pH-shift stressed did not contain these resistant fragments and the overall tone of the oxidized sample lane remained darker for sizes below 150 kDa than the tone of other lanes.…”

Specific Antibody Responses to Subtilisin Carlsberg (Alcalase) in Mice: Development of an Intranasal Exposure Model

“…Different inbred strains of mice will respond differently depending on the immunogen, the adjuvant, the antibody isotype of interest, and the route of administration (Tomlinson et al, 1989;Else and Wakelin, 1989;Gurvich and Korukova, 1986;Nakashima et al, 1983;Revoltella and Ovary, 1969;Mancino and Ovary, 1980;Staruch and Wood, 1982;Kudo et al, 1978;Petty and Steward, 1977;Berzofsky et al, 1977;Fujiwara et al, 1976;Nomoto et al, 1972). Though Balb/c mice have been good responders in other systems, including the i.t.…”