Strain improvement of chymosin-producing strains of Aspergillus niger var. awamori using parasexual recombination

Bodie, Elizabeth A.; Armstrong, Gale; Dunn-Coleman, Nigel

doi:10.1016/0141-0229(94)90151-1

Cited by 39 publications

(12 citation statements)

References 17 publications

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“…Further improvement was done by parasexual recombination resulting in a strain producing 1.5 g/l from parents producing 1.2 g/l [79]. Four recombinant proteases have been approved by FDA for cheese production [80,81].…”

Section: Improving Productionmentioning

confidence: 99%

Contributions of Microorganisms to Industrial Biology

2007

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Life on earth is not possible without microorganisms. Microbes have contributed to industrial science for over 100 years. They have given us diversity in enzymatic content and metabolic pathways. The advent of recombinant DNA brought many changes to industrial microbiology. New expression systems have been developed, biosynthetic pathways have been modified by metabolic engineering to give new metabolites, and directed evolution has provided enzymes with modified selectability, improved catalytic activity and stability. More and more genomes of industrial microorganisms are being sequenced giving valuable information about the genetic and enzymatic makeup of these valuable forms of life. Major tools such as functional genomics, proteomics, and metabolomics are being exploited for the discovery of new valuable small molecules for medicine and enzymes for catalysis.

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Section: Improving Productionmentioning

confidence: 99%

Contributions of Microorganisms to Industrial Biology

2007

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“…Chymosin, e.g., cleaves a peptide bond in casein and is the crucial enzyme coagulant in the manufacture of cheese. It is traditionally derived from animal rennets, but attempts to more efficiently produce chymosin in microorganisms such as Kluyveromyces lactis (Swinkels et al 1993) and Aspergillus (Bodie et al 1994) have led to a number of new recombinant chymosins on the market.…”

Section: Proteases and Peptidasesmentioning

confidence: 99%

Technical Enzyme Production and Whole‐Cell Biocatalysis: Application of Hansenula polymorpha

Papendieck¹,

Dahlems²,

Gellissen³

2002

Hansenula Polymorpha

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“…In order to increase the production of different metabolites by Aspergillus strains, parasexual crosses between selected strains may be considered. This strategy has been used to enhance the production levels of metabolites, enzymes [5], recombinant proteins [13] and citric acid [14].…”

Section: Introductionmentioning

confidence: 99%