Strain-specific probiotic (Lactobacillus helveticus) inhibition ofCampylobacter jejuni invasion of human intestinal epithelial cells

Abstract

Campylobacter jejuni is the most common bacterial cause of enterocolitis in humans, leading to diarrhoea and chronic extraintestinal diseases. Although probiotics are effective in preventing other enteric infections, beneficial microorganisms have not been extensively studied with C. jejuni. The aim of this study was to delineate the ability of selected probiotic Lactobacillus strains to reduce epithelial cell invasion by C. jejuni. Human colon T84 and embryonic intestine 407 epithelial cells were pretreated with Lactobacillus strains and then infected with two prototypic C. jejuni pathogens. Lactobacillus helveticus, strain R0052 reduced C. jejuni invasion into T84 cells by 35-41%, whereas Lactobacillus rhamnosus R0011 did not reduce pathogen invasion. Lactobacillus helveticus R0052 also decreased invasion of one C. jejuni isolate (strain 11168) into intestine 407 cells by 55%. Lactobacillus helveticus R0052 adhered to both epithelial cell types, which suggest that competitive exclusion could contribute to protection by probiotics. Taken together, these findings indicate that the ability of selected probiotics to prevent C. jejuni-mediated disease pathogenesis depends on the pathogen strain, probiotic strain and the epithelial cell type selected. The data support the concept of probiotic strain selectivity, which is dependent on the setting in which it is being evaluated and tested.

Probiotic Colonization of the Adherent Mucus Layer of HT29MTXE12 Cells Attenuates Campylobacter jejuni Virulence Properties

“…Animal and human studies of the effects of probiotics on the gut involve at least a period of persistent colonization for the effects to be observed (33). Despite this, most in vitro studies of probiotic action typically involve short exposure of model epithelia to the probiotic strains used (1,19,25,46). It is possible that such brief pretreatment of cells with probiotics fails to reproduce at least some of the effects that would be seen in vivo.…”

“…Preliminary data from coculture (7) and animal studies (29,41,45) point to the potential effectiveness of probiotics in inhibiting C. jejuni infection. In a recent paper, Wine et al (46) provide evidence for strain-and cell-type-specific reduction in C. jejuni invasiveness following coculture with probiotics. However, in general, the effect of probiotics on the pathogenicity of C. jejuni has received little attention.…”

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

“…Furthermore, prevention of disease in humans and a reduction of the pathogen reservoir in farm animals, without the need for antibiotics, are of both ecological and financial benefit to society. In recent years, the beneficial effects of probiotic use on animal health, on the reduction of pathogens in the food chain, and on growth performance have increasingly been highlighted (Wine et al, 2009;Modesto et al, 2009;Patterson & Burkholder, 2003).…”

Labneh with probiotic properties produced from kefir: development and sensory evaluation

“…These mechanisms lead to the modulation of the microbiota and/or inhibition of colonization by potential pathogens, and bacterial translocation across the intestinal wall (Eizaguirre et al, 2011;Wine et al, 2009;Wong et al, 2013).…”