1993
DOI: 10.1128/jvi.67.10.6179-6191.1993
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Strain specificity and binding affinity requirements of neutralizing monoclonal antibodies to the C4 domain of gp120 from human immunodeficiency virus type 1

Abstract: The binding properties of seven CD4-blocking monoclonal antibodies raised against recombinant gpl20 of human immunodeficiency virus type 1 strain MN (HWV-lMN) and two CD4-blocking monoclonal antibodies to recombinant envelope glycoproteins gpl20 and gpl60 of substrain IIIB of HIVLm were analyzed. With a panel of recombinant gpl20s from seven diverse HIV-1 isolates, eight of the nine antibodies were found to be strain specific and one was broadly cross-reactive. Epitope mapping revealed that all nine antibodies… Show more

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Cited by 42 publications
(26 citation statements)
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“…Antibodies to the CD4bd have neutralizing activity, but anti-CD4bd MAbs are not capable of neutralizing all HIV-1 strains; thus, of a panel of three human anti-CD4bd MAbs tested against eight type B HIV-1 laboratory isolates, none was able to neutralize all vari-ants (16). Similar results were found by others using both laboratory and primary isolates of HIV-1 (2,24,39,44,51).…”
supporting
confidence: 76%
See 1 more Smart Citation
“…Antibodies to the CD4bd have neutralizing activity, but anti-CD4bd MAbs are not capable of neutralizing all HIV-1 strains; thus, of a panel of three human anti-CD4bd MAbs tested against eight type B HIV-1 laboratory isolates, none was able to neutralize all vari-ants (16). Similar results were found by others using both laboratory and primary isolates of HIV-1 (2,24,39,44,51).…”
supporting
confidence: 76%
“…At a minimum, one can state that shared antigens of the CD4bd are found among most of the clades (35), but the degree to which they are shared by individual viruses cannot be ascertained by staining infected PBMCs. Previous studies of virus neutralization (2,16,24,39,44,51) showed that only a portion of viruses studied can be neutralized by anti-CD4bd MAbs, suggesting that, on this functional criterion, some antigens of this large and complex epitope may fall into the variant-specific rather than the groupspecific category.…”
Section: Discussionmentioning
confidence: 94%
“…Conversely, the presence of a MAb epitope on monomeric gp120 from a primary virus does not predict that the virus will be neutralized. The affinities of certain antibodies for monomeric gp120 from a T-cell line-adapted strain such as IIIB or MN can be used to predict their virus neutralization potencies (4,54). However, this relationship is not found for other antibodies (60), and it does not appear to extend generally to primary viruses, as discussed above for 19b.…”
Section: Discussionmentioning
confidence: 99%
“…In order to maintain as much tertiary structure as possible, the constructs were designed to preserve the disulfide bridges found in the full-length gp140. As described previously (Nakamura et al, 1993(Nakamura et al, , 2012, fragments expressed by this approach are typically glycosylated, and maintain the disulfide structures required for recognition by a variety of conformation-dependent mAbs.…”
Section: Immunization With 108060 Q655r Rgp140 and Initial Mab Screeningmentioning
confidence: 99%