Strand specific transcription of satellite DNA I in rat ascites hepatoma cells

Miyahara, Masaki; Sumiyoshi, Hideo; Yamamoto, Mikio; Endo, Hideki

doi:10.1016/0006-291x(85)90501-7

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…However, in computational analysis, we analyzed only the quantity of polyadenylated transcripts to avoid contamination with the genome DNA (see "Materials and methods"). Pericentromeric DNA transcripts are known to be polyadenylated in human and mouse 12,42 though the existence of non-polyadenylated satellite RNA was demonstrated in a rat ascites hepatoma cell line 47 .…”

Section: Discussionmentioning

confidence: 99%

Human pericentromeric tandemly repeated DNA is transcribed at the end of oocyte maturation and is associated with membraneless mitochondria-associated structures

Dobrynin

Korchagina

Prjibelski

et al. 2020

Sci Rep

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Most of the human genome is non-coding. However, some of the non-coding part is transcriptionally active. In humans, the tandemly repeated (TR) pericentromeric non-coding DNA—human satellites 2 and 3 (HS2, HS3)—are transcribed in somatic cells. These transcripts are also found in pre- and post-implantation embryos. The aim of this study was to analyze HS2/HS3 transcription and cellular localization of transcripts in human maturating oocytes. The maternal HS2/HS3 TR transcripts transcribed from both strands were accumulated in the ooplasm in GV-MI oocytes as shown by DNA–RNA FISH (fluorescence in-situ hybridization). The transcripts’ content was higher in GV oocytes than in somatic cumulus cells according to real-time PCR. Using bioinformatics analysis, we demonstrated the presence of polyadenylated HS2 and HS3 RNAs in datasets of GV and MII oocyte transcriptomes. The transcripts shared a high degree of homology with HS2, HS3 transcripts previously observed in cancer cells. The HS2/HS3 transcripts were revealed by a combination of FISH and immunocytochemical staining within membraneless RNP structures that contained DEAD-box helicases DDX5 and DDX4. The RNP structures were closely associated with mitochondria, and are therefore similar to membraneless bodies described previously only in oogonia. These membraneless structures may be a site for spatial sequestration of RNAs and proteins in both maturating oocytes and cancer cells.

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Section: Discussionmentioning

confidence: 99%

Human pericentromeric tandemly repeated DNA is transcribed at the end of oocyte maturation and is associated with membraneless mitochondria-associated structures

Dobrynin

Korchagina

Prjibelski

et al. 2020

Sci Rep

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“…In the past few years, it has become evident that constitutive heterochromatin may indeed have a function. Several studies describing transcription of satellite sequences in var-ious organisms point toward a more active role of these regions (Diaz et al, 1981;Miyahara et al, 1985;Epstein et al, 1986;Schafer et al, 1986;Wu et al, 1986;Bonaccorsi et al, 1990;Gaubatz and Cutler, 1990;Belyaeva et al, 1992;Rudert et al, 1995;Rouleux-Bonnin et al, 1996;Renault et al, 1999). Work in yeast indicates the involvement of transcripts of repetitive DNA in silencing of heterochromatin regions (Volpe et al, 2002), whereas the large noncoding X-IST transcript is a key player in the process of X-chromosome inactivation in mammalian females (Boumil and Lee, 2001).…”

Section: Introductionmentioning

confidence: 99%

Structural and Functional Characterization of Noncoding Repetitive RNAs Transcribed in Stressed Human Cells

Valgardsdóttir

Chiodi

Giordano

et al. 2005

MBoC

111

108

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Thermal and chemical stresses induce the formation in human cells of novel and transient nuclear structures called nuclear stress bodies (nSBs). These contain heat shock factor 1 (HSF-1) and a specific subset of pre-mRNA processing factors. Nuclear stress bodies are assembled on specific pericentromeric heterochromatic domains containing satellite III (SatIII) DNA. In response to stress, these domains change their epigenetic status from heterochromatin to euchromatin and are transcribed in poly-adenylated RNAs that remain associated with nSBs. In this article, we describe the cloning, sequencing, and functional characterization of these transcripts. They are composed of SatIII repeats and originate from the transcription of multiple sites within the SatIII arrays. Interestingly, the level of SatIII RNAs can be down-regulated both by antisense oligonucleotides and small interfering RNAs (siRNA). Knockdown of SatIII RNA by siRNAs requires the activity of Argonaute 2, a component of the RNA-induced silencing complex. Down-regulation of satellite III RNAs significantly affects the recruitment of RNA processing factors to nSBs without altering the association of HSF-1 with these structures nor the presence of acetylated histones within nSBs. Thus, satellite III RNAs have a major role in the formation of nSBs.

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The Role of ncRNA in Centromeres: A Lesson from Marsupials

O’Neill

Carone

2009

Centromere

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Though centromeres have been thought to be comprised of repetitive, transcriptionally inactive DNA, new evidence suggests that eukaryotic centromeres produce a variety of transcripts and that RNA is essential for centromere competence. It has been proposed that centromere satellite transcripts play an essential role in centromere function through demarcation of the kinetochore-binding domain. However, the regional limits and regulation of transcription within the mammalian centromere are unknown. Analysis of transcriptional domains within the centromere in mammalian models is impeded by the unbridgeable expanse of satellite monomers throughout the pericentromere. The comparatively small size of the wallaby centromere and the evolutionary role of the centromere in marsupial speciation events position the wallaby centromere as a tractable and valuable mammalian centromere model. We highlight the current understanding of the wallaby centromere and the role of transcription in centromere function.

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Strand specific transcription of satellite DNA I in rat ascites hepatoma cells

Cited by 5 publications

References 15 publications

Human pericentromeric tandemly repeated DNA is transcribed at the end of oocyte maturation and is associated with membraneless mitochondria-associated structures

Human pericentromeric tandemly repeated DNA is transcribed at the end of oocyte maturation and is associated with membraneless mitochondria-associated structures

Structural and Functional Characterization of Noncoding Repetitive RNAs Transcribed in Stressed Human Cells

The Role of ncRNA in Centromeres: A Lesson from Marsupials

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