Strategic biocatalysis with hyperthermophilic enzymes

Comfort, Donald A.; Chhabra, Swapnil R.; Conners, Shannon B.; Chou, Chin Cheng; Epting, Kevin L.; Johnson, Matthew R.; Jones, Kristen L.; Sehgal, Amitabh C.; Kelly, Robert M.

doi:10.1039/b406297c

Cited by 34 publications

(13 citation statements)

References 83 publications

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“…Deciphering these secrets would enable us to exploit mannanases for their applications in biotechnology. The following section will discuss details of some of the most promising applications of mannanases (Howard et al, 2003;Galbe et al, 2002;Comfort et al, 2004).…”

Section: Applicationsmentioning

confidence: 99%

Microbial Mannanases: An Overview of Production and Applications

Dhawan¹,

Kaur

2007

Critical Reviews in Biotechnology

388

271

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Microbial mannanases have become biotechnologically important since they target the hydrolysis of complex polysaccharides of plant tissues into simple molecules like manno-oligosaccharides and mannoses. The role of mannanases in the paper and pulp industry is well established and recently they have found application in the food and feed technology, coffee extraction, oil drilling and detergent industry. Mannanses are enzymes produced mainly from microorganisms but mannanases produced from plants and animals have also been reported. Bacterial mannanases are mostly extracellular and can act in a wide range of pH and temperature, though acidic and neutral mannanases are more common. This review will focus on complex mannan structure and the microbial enzyme complex involved in its complete breakdown, mannanase sources, production conditions and their applications in the commercial sector. The reference to plant and animal mannanases has been made to complete the overview. However, the major emphasis of the review is on the microbial mannanases.

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Section: Applicationsmentioning

confidence: 99%

Microbial Mannanases: An Overview of Production and Applications

Dhawan¹,

Kaur

2007

Critical Reviews in Biotechnology

388

271

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“…␤-Mannanases and ␣-galactosidases serve as enzyme breakers that hydrolyse the galactomannans in the fracturing fluid. Thermostable enzymes are of particular interest in this application due to geothermal gradients in deep oil wells [115]. In addition, premature hydrolysis of galactomannans at the earth's surface is prevented when using hyperthermophilic enzymes as surroundings only reach optimal temperatures for enzyme activity deep in the well [116].…”

Section: Oil and Gas Well Stimulationmentioning

confidence: 99%

Fungal β-mannanases: Mannan hydrolysis, heterologous production and biotechnological applications

Zyl

Rose

Trollope

et al. 2010

Process Biochemistry

166

108

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“…Di sisi lain, terdapat enzim hidrolitik mananase (EC 3.2.1.x) yang secara acak memotong ikatan  1,4 pada rantai utama gl ukomanan, galaktomanan, dan galaktoglukomanan (Collins, Gerday & Feller, 2005;McCleary, 1988). Enzim xilanase dan mananase banyak diaplikasikan dalam kegiatan industri, seperti untuk kebutuhan pakan, pangan, energi, hingga pembuatan kertas (Chen, Ko, Huang & Guo, 2015;Comfort et al, 2004;Dhawan & Kaur, 2007;Khasin, Alchanati & Shoham, 1993).…”

Section: Pendahuluanunclassified

“…isolate and (e & f) L22207 isolate minyak dan gas selama proses pengeboran (Comfort et al, 2004). Aktivitas enzim kasar xilanase dan mananase pada isolat terpilih dari hari pertama hingga hari kelima diukur dengan metode DNS.…”

Section: Optimization Of Ph and Temperature Of Mannolytic Isolates unclassified

Isolasi Bakteri Pendegradasi Xilan dan Manan dari Perairan Indonesia

Tasia¹,

Zuraida

Yopi

2016

JPBKP

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ABSTRAKBakteri laut penghasil enzim xilanase dan mananase menyimpan banyak potensi bagi bioteknologi kelautan. Informasi sebarannya juga dapat digunakan untuk memetakan keragaman bakteri laut serta analisis lingkungan perairan Indonesia sehingga pemanfaatannya dapat tepat sasaran. Penelitian ini bertujuan untuk mengisolasi bakteri laut pendegradasi xilan dan manan dari Indonesia. Penapisan kemampuan xilanolitik dan manolitik menggunakan metode Congo red dilakukan pada isolat bakteri yang diisolasi dari Laut Jawa, Selat Makassar, Laut Flores, dan Laut Sawu pada kedalaman 5 dan 20 m. Hasil penelitian menunjukkan bahwa isolat X7517, X1654, dan XM26511 diketahui menghasilkan xilanase dengan kondisi optimal reaksi enzim masing-masing adalah pH 9, T 90 °C (2,253±2,075 U/ml), pH 6, T 70 °C (0,633±0,082 U/ml), dan pH 6, T 70 °C (2,293±0,066 U/ml). Ketiganya diketahui memiliki kesamaan genetis dengan Halomonas aquamarina DSM 30161, Alteromonas macleodii NBRC 102226, dan H. meridiana NBRC 15608. Isolat bakteri manolitik L15203 dan L16571 memiliki kesamaan dengan Idiomarina zobellii KMM231, sedangkan isolat L2207 memiliki kesamaan dengan Bacillus sp. MB 71. Ketiga bakteri tersebut memiliki aktivitas mananase optimal pada kondisi alkali, masing-masing pada pH 8, T 80 °C (0,477±0,024 U/ml) untuk L 15203, pH 9, T 90 °C (0,476±0,009U/ml) untuk L16571 dan pH 9, T 80 °C (0,528±0,057 U/ml) untuk L2207. Bakteri laut xilanolitik dan manolitik yang berpotensi dalam produksi xilanase dan mananase melimpah di sebagian perairan Indonesia, terutama di perairan yang semakin dekat permukaan laut atau perairan dangkal. 8, T 70 °C (0.477±0,024 U/ml), pH 9, T 80 °C (0.476±0,009U/ml) and pH 9, T 80 °C (0.528 ±0,057 U/ml), respectively. Xylanolytic and mannolytic marine bacteria that are potential for xylanase and mannanase production were found abundantly on parts of Indonesian waters particularly at shallow water. KATA KUNCI: bakteri laut Indonesia, xilanase, mananase ABSTRACT Xylanase and mannanase producing marine bacteria are potential to be utilized in marine biotechnology. Information of their distribution is important to determine the biodiversity pattern of marine bacteria and to analyze the aquatic environment in

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Strategic biocatalysis with hyperthermophilic enzymes

Cited by 34 publications

References 83 publications

Microbial Mannanases: An Overview of Production and Applications

Microbial Mannanases: An Overview of Production and Applications

Fungal β-mannanases: Mannan hydrolysis, heterologous production and biotechnological applications

Isolasi Bakteri Pendegradasi Xilan dan Manan dari Perairan Indonesia

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