Strategic design of a Mussel-inspired in situ reduced Ag/Au-Nanoparticle Coated Magnesium Alloy for enhanced viability, antibacterial property and decelerated corrosion rates for degradable implant Applications

Abstract

Magnesium (Mg) and its alloys have attracted much attention as a promising candidate for degradable implant applications however the rapid corrosion of magnesium inside the human body greatly limits its use as an implant material. Therefore, coating the alloy surface with a multifunctional film is a promising way to overcome the drawbacks. Here we propose for the first time a multifunction layer coating to enhance the cell viability, antibacterial property and decelerated corrosion rates to act as a novel material to be used for degradable implant Applications. For that, the magnesium alloy (AZ31) was first treated with hydrofluoric acid (HF) and then dopamine tris Hydrochloric acid (tris-HCL) solution. The reducing catechol groups in the polydopamine (PD) layer subsequently immobilize silver/gold ions in situ to form uniformly dispersed Ag/Au nanoparticles on the coating layer. The successful formation of Ag/Au nanoparticles on the HF-PD AZ31 alloy was confirmed using XPS and XRD, and the morphology of all the coated samples were investigated using SEM images. The alloy with HF-PDA exhibit enhanced cell attachment and proliferation. Moreover, the nanoparticle immobilized HF-PD alloy exhibited dramatic corrosion resistance enhancement with superior antibacterial properties and accountable biocompatibility. Thus the result suggest that HF-PD Ag/Au alloy has great potential in the application of degradable implant and the surface modification method is of great significance to determine its properties.

Advances in Antibacterial Functionalized Coatings on Mg and Its Alloys for Medical Use—A Review

“…It can be seen from the table that multifunctional coatings with the properties of good corrosion resistance and excellent antibacterial ability are combination with a verity of materials. Biodegradable polymers with good biocompatibility such as polydopamine (PD) [24], polycaprolactone (PCL) [67,97], poly (D, L-lactic acid) [63,64] are the most frequently used coating materials. Moreover, many studies were concentrated on dipping [24,53,102,106], layer-by-layer (LbL) assembly [58], and electrophoretic deposition [31,109] to combine the properties of different polymers.…”

“…Typical studies on in vitro cytocompatibility are shown in Table 6. In cell studies, the cytocompatibility and positive effects of antibacterial coatings on cell proliferation were frequently shown for bone-linked cells such as murine osteoblast-like cell line MC3T3-E1 [18][19][20]24,27] and human osteoblast cell line MG-63 [67,96]. Besides proliferation, cell adhesion and osteoblast differentiation were also evaluated in most of the studies.…”

“…Most of the research studies were carried out by adding antibiotics [21][22][23] or antibacterial metallic elements (silver, copper, zinc, etc.) [24][25][26] into polymer coatings [27][28][29][30] or calcium phosphates (Cap) coatings [31,32]. Furthermore, many physical and chemical surface modification strategies, such as dipping, plasma electrolytic oxidation (PEO), Lay-by-Lay (LbL) assembly, and Sol-Gel, were adopted to enhance the combination properties of the antibacterial coatings.…”

A Novel Design of Tri-Layer Membrane with Controlled Delivery of Paclitaxel and Anti-Biofilm Effect for Biliary Stent Applications

“…About 60% of early stent occlusion is caused by biliary clogging and sludge, and continuous effort has been made to address such type of stenting failure with respect to minimizing microbial adhesion and biofilm formation [ 15 , 16 ]. Previous studies suggest that both systemic and local administration of antibiotics and choleretics have little effect in preventing biofilm formation as well as improving the patency [ 17 , 18 , 19 ]. Many alternative approaches have been proposed to facilitate drainage, ranging from surface modification to immobilization of biomolecules or biocompatible materials [ 20 ].…”

Bi-layered Nanofibers Membrane Loaded with Titanium Oxide and Tetracycline as Controlled Drug Delivery System for Wound Dressing Applications

“…In particular, PCL nanofibrous membranes fabricated by electrospinning are attracting a lot of interest for various applications, because they have numerous useful properties including high surface area, biodegradability, biocompatibility, and excellent mechanical properties [22]. PDO composite nanofibrous mat-loaded antibacterial agents, such as Ag nanoparticles, ciprofloxacin and tetracycline, are attractive wound dressing materials due to their excellent antibacterial activity against Gram-positive and Gram-negative bacteria [23,24,25]. Tetracycline (TTC) is one of the most commonly antibiotics for the treatment of several skin infections [26], such as acne, periodontal, and urinary infections.…”