Strategies for Enhanced Production of Plant Secondary Metabolites from Cell and Organ Cultures

Murthy, Hosakatte Niranjana; Dandin, Vijayalaxmi S.; Zhong, Jian‐Jiang; Paek, Kee-Yoeup

doi:10.1007/978-94-017-9223-3_20

Cited by 41 publications

(20 citation statements)

References 143 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These phytochemicals, varying according to plant species, variety, plant organ, growing stage, harvest time and postharvest processes and also prevailing climatic and soil factors, are greatly affected positively or negatively by the environmental conditions surrounding the plants. In this case, the produced compounds are hardly difficult to be in the same quality standards (Murthy et al, 2014;Gehlot et al, 2017). Cell suspension cultures, one of the most common tissue culture applications, may lead to obtain these compounds in specific quality standards, generate new compounds not found in original plant, and produce commercially these economically highly valuable phytochemicals independently of ecological conditions (Srivastava et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Açıkgöz¹,

Kara²,

AY³

et al. 2018

Akademik Ziraat Dergisi

View full text Add to dashboard Cite

Light is not only effective in photosynthesis, growth and development, but also play an important role in biosynthesis of primary and secondary metabolites. The present research was aimed to determine effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in cell suspension cultures of Echinacea purpurea L. Stem explants derived from sterile plants were subjected to callus culture in a B5 media supplemented with 1.0 mg/L BAP and 2.0 mg/L NAA. Afterward, 8-day cell cultures were incubated in light and dark environments and cell harvesting, with a three-day interval, was carried out five times. The contents of alkamide, caffeic acid derivatives and echinacoside were determined using HPLC. Alkamide, caftaric acid and echinacoside content increased regularly with light application. Light application increased alkamide content by 57%, as an average, compared to darkness. The cell cultures exposed to 12 days of incubation in light produced the highest alkamide content, with a 2.11-fold increase, compared to dark. Subjecting the cell cultures to light for 12 days resulted in 70% and 63% increases in caftaric acid and echinacoside. This study showed that light application in Echinacea purpurea L. cell suspension culture had a great potential for increasing some important phytochemicals.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Açıkgöz¹,

Kara²,

AY³

et al. 2018

Akademik Ziraat Dergisi

View full text Add to dashboard Cite

show abstract

“…In the case of isoquinoline alkaloid biosynthesis, the light could alter the production of their precursor tyrosine, in which biosynthesis is dependent on the photosynthesis [31,32]. Moreover, light could act not only as energy source for photosynthesis but also as powerful abiotic elicitor of secondary metabolism in plant cells [21]. In this study, we investigated the influence of light on the growth and protopine biosynthesis by selected cell suspensions of F. rostellata and F. officinalis.…”

Section: Effect Of Light On Growth and Protopine Biosynthesis By F Rmentioning

confidence: 99%

“…Development of such in vitro cultures would be a powerful tool for fundamental study of their metabolism and for the commercial production of protopine. It is well known that the light could have a tremendous impact on secondary metabolism of plant cells [20,21]. Study on the effect of light on protopine accumulation in Fumaria in vitro cells may provide a basic tool for manipulating the yields and creating eco-friendly biotechnology for its production.…”

Section: Introductionmentioning

confidence: 99%

Protopine Production by Fumaria Cell Suspension Cultures: Effect of Light

Georgieva

Ivanov

Marchev

et al. 2015

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Protopine biosynthesis in Fumaria rostellata and Fumaria officinalis cell suspensions was investigated. For the first time, we reported for calli and cell suspensions obtained from F. rostellata and F. officinalis. Callus induction was initiated on a Murashige and Skoog medium, supplemented with sucrose and various concentrations of plant growth regulators: 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The best morphological characteristics, growth behavior, and protopine biosynthesis were observed for two callus lines (5FRL14 and 12FOL1) cultivated under submerged conditions, at low concentration of 2,4-D (0.2 and 0.5 mg/L) and higher concentration of BAP (2.0 and 3.0 mg/L). The maximal yield of protopine was accumulated from cell suspension of F. rostellata (line 5FRL14) cultivated under illumination-49.6 mg/L. Time courses of utilization of sucrose, ammonium, nitrate, and phosphate ions in cultural liquid and acetylcholinesterase inhibitory activity of alkaloid extracts of studied suspensions are also presented.

show abstract

“…These results have pragmatic inferences for cell suspension culture aimed at increasing the levels of polyphenols in date cultivars. Secondary metabolite accumulation is genotypic dependent in plants which leads to variation in the metabolites accumulation among cultivars (Murthy et al, 2014;Hamad et al, 2015).…”

Section: Cell Line Selectionmentioning

confidence: 99%

Cell suspension culture as a means to produce polyphenols from date palm (Phoenix dactylifera L.)

Naik

Al-Khayri

2018

Ciênc. agrotec.

View full text Add to dashboard Cite

Date palm accumulates a wide range of secondary metabolites high in nutritional and therapeutic value. In the present study, date palm (Phoenix dactylifera L., cv. Shaishi) shoot-tip-induced callus was used to establish cell suspension cultures in Murashige and Skoog (MS) liquid medium containing 1.5 mg L-1 2-isopentenyladenine (2iP) and 10 mg L-1 naphthaleneacetic acid (NAA). To study the growth kinetics, cultures were maintained for 12 weeks during which weekly measurements were carried out to determine the biomass accumulation based on packed cell volume (%), fresh weight and dry weight (g). In addition, weekly determination of polyphenols (catechin, caffeic acid, kaempferol, and apigenin) was carried out using high performance liquid chromatography (HPLC). The 11-week-old culture was found highest in the production of biomass (62.9 g L-1 fresh weight and 7.6 g L-1 dry weight) and polyphenols (catechin-155.9 µg L-1, caffeic acid-162.7 µg L-1, kaempferol-89.7 µg L-1, and apigenin-242.7 µg L-1) from the cell suspension cultures. This is the first report on the production of polyphenols from the cell suspension culture of date palm. This study facilitates further development of large-scale production of polyphenols and the utilization of bioreactors.

show abstract

Strategies for Enhanced Production of Plant Secondary Metabolites from Cell and Organ Cultures

Cited by 41 publications

References 143 publications

Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Protopine Production by Fumaria Cell Suspension Cultures: Effect of Light

Cell suspension culture as a means to produce polyphenols from date palm (Phoenix dactylifera L.)

Contact Info

Product

Resources

About