Strategies for Global RNA Sequencing of the Human Pathogen Neisseria gonorrhoeae

Identification of a GrgA-Euo-HrcA Transcriptional Regulatory Network in Chlamydia

“…Subsequent RNA-seq studies were conducted with samples harvested from infected L929 cells at 16 hpi. We chose this time point first because it corresponds to the mid-log phase of RB replication, and we are most interested in uncovering how GrgA mediates RB growth regulation, and additionally because the genome coverages in the above pilot study with samples prepared at 16 hpi exceeded the recommended coverage for bacteria with small genomes ( 42 ). We repeated RNA-seq analysis with ATC-treated biological replicates for the 12 to 16 hpi time period to generate data that could be analyzed statistically.…”