Strategies for Pyramiding Resistance Genes Against the Barley Yellow Mosaic Virus Complex (BaMMV, BaYMV, BaYMV-2)

Werner, Kay; Friedt, Wolfgang; Ordon, Frank

doi:10.1007/s11032-005-3445-2

Cited by 132 publications

(76 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its implementation may, however, reduce the potential for the emergence of resistance-breaking viral isolates. Pyramiding several resistance genes in a single genotype is considered to be a promising strategy to obtain durable virus resistance (39). With the identification of rym11 as loss-of-function alleles of HvPDIL5-1, it is now possible to combine two recessive resistance loci (rym11 and rym4/rym5) on the basis of perfectly linked and diagnostic molecular markers.…”

Section: Loss-of-function Alleles Are Prevalent In Domesticated Barleymentioning

confidence: 99%

PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

Yang

Lüpken

Habekuß

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Protein disulfide isomerases (PDIs) catalyze the correct folding of proteins and prevent the aggregation of unfolded or partially folded precursors. Whereas suppression of members of the PDI gene family can delay replication of several human and animal viruses (e.g., HIV), their role in interactions with plant viruses is largely unknown. Here, using a positional cloning strategy we identified variants of PROTEIN DISULFIDE ISOMERASE LIKE 5-1 (HvPDIL5-1) as the cause of naturally occurring resistance to multiple strains of Bymoviruses. The role of wild-type HvPDIL5-1 in conferring susceptibility was confirmed by targeting induced local lesions in genomes for induced mutant alleles, transgene-induced complementation, and allelism tests using different natural resistance alleles. The geographical distribution of natural genetic variants of HvPDIL5-1 revealed the origin of resistance conferring alleles in domesticated barley in Eastern Asia. Higher sequence diversity was correlated with areas with increased pathogen diversity suggesting adaptive selection for bymovirus resistance. HvPDIL5-1 homologs are highly conserved across species of the plant and animal kingdoms implying that orthologs of HvPDIL5-1 or other closely related members of the PDI gene family may be potential susceptibility factors to viruses in other eukaryotic species.allele mining | resistance breeding | soil-borne virus disease | chaperone

show abstract

Section: Loss-of-function Alleles Are Prevalent In Domesticated Barleymentioning

confidence: 99%

PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

Yang

Lüpken

Habekuß

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The successful pyramiding of disease resistance genes or resistance QTLs and the effectiveness of this strategy has been reported for example for stripe rust (Castro et al 2003) or barley yellow mosaic virus resistance (Okada et al 2004, Werner et al 2005 in barley, leaf rust (Kloppers and Pretorius 1997) or powdery mildew resistance (Liu et al 2000) in wheat, as well as leaf blast (Hittalmani et al 2000) or bacterial blight resistance (Shanti et al 2001;Singh et al 2001) in rice.…”

Section: Diagnostic Molecular Markersmentioning

confidence: 99%

Fine mapping, physical mapping and development of diagnostic markers for the Rrs2 scald resistance gene in barley

et al. 2009

View full text Add to dashboard Cite

show abstract

“…tolerance means that unrestricted virus infection and replication takes place but infected plants show only mild or no symptoms and no or minor decrease in grain yield, while resistance is characterised by a decreased virus replication in infected plants. In order to determine whether combining some of the above mentioned loci leads to a higher level of tolerance against a German isolate of BYDV-PAV, Ryd2, Ryd3 and the QTL on chromosome 2HL were combined using DH-lines and molecular markers, a strategy which was eYcient in pyramiding diVerent resistance genes against the barley yellow mosaic virus complex (Werner et al 2005(Werner et al , 2007.…”

Section: Introductionmentioning

confidence: 99%

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

et al. 2011

Self Cite

View full text Add to dashboard Cite

Barley yellow dwarf virus (BYDV) is an economically important pathogen of barley, which may become even more important due to global warming. In barley, several loci conferring tolerance to BYDV-PAV-ASL-1 are known, e.g. Ryd2, Ryd3 and a quantitative trait locus (QTL) on chromosome 2H. The aim of the present study was to get information whether the level of tolerance against this isolate of BYDV in barley can be improved by combining these loci. Therefore, a winter and a spring barley population of doubled haploid (DH) lines were genotyped by molecular markers for the presence of the susceptibility or the resistance encoding allele at respective loci (Ryd2, Ryd3, QTL on chromosome 2H) and were tested for their level of BYDV-tolerance after inoculation with viruliferous (BYDV-PAV-ASL-1) aphids in Weld trials. In DH-lines carrying the combination Ryd2 and Ryd3, a signiWcant reduction of the virus titre was detected as compared to lines carrying only one of these genes. Furthermore, spring barley DH-lines with this allele combination also showed a signiWcantly higher relative grain yield as compared to lines carrying only Ryd2 or Ryd3. The QTL on chromosome 2H had only a small eVect on the level of tolerance in those lines carrying only Ryd2, or Ryd3 or a combination of both, but the eVect in comparison to lines carrying no tolerance allele was signiWcant. Overall, these results show that the combination of Ryd2 and Ryd3 leads to quantitative resistance against BYDV-PAV instead of tolerance.

show abstract

Strategies for Pyramiding Resistance Genes Against the Barley Yellow Mosaic Virus Complex (BaMMV, BaYMV, BaYMV-2)

Cited by 132 publications

References 33 publications

PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

Fine mapping, physical mapping and development of diagnostic markers for the Rrs2 scald resistance gene in barley

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

Contact Info

Product

Resources

About