Strategies for the photo-control of endogenous protein activity

Brechun, Katherine E.; Arndt, Katja M.; Woolley, G. Andrew

doi:10.1016/j.sbi.2016.11.014

Cited by 16 publications

(20 citation statements)

References 42 publications

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“…In addition to transcriptional control, biological circuits can also be tuned by offering direct control over protein activities (Table 1 ). Optical control of protein activities has been established in eukaryotic cells in part due to the access to a wide range of sensors (Brechun et al, 2017 ; Liu and Tucker, 2017 ). These sensors belong to one-component systems which undergo conformational changes or shifts in their dimerization or oligomerization states upon light illumination.…”

Section: Light In Tuning Synthetic Circuitsmentioning

confidence: 99%

“…Light has also been used to control subcellular localizations of proteins (Brechun et al, 2017 ). One strategy is based on separated light sensors which can be brought together upon illumination, including Phy/PIF and magnets.…”

Section: Light In Tuning Synthetic Circuitsmentioning

confidence: 99%

“…In one study, protein localizations to nucleus, endosomes, and cell membrane were achieved by the PhyB/PIF6 system (Yang et al, 2013 ). Another strategy is to fuse LOV family proteins to cage the signal peptide (Brechun et al, 2017 ). Different systems have been engineered to control import to and export from the nucleus (Di Ventura and Kuhlman, 2016 ; Yumerefendi et al, 2016 ) and a recent study has proposed a strategy to control tridirectional protein localization among the nucleus, cytoplasm, and plasma membrane (Redchuk et al, 2018a ).…”

Section: Light In Tuning Synthetic Circuitsmentioning

confidence: 99%

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Programming Bacteria With Light—Sensors and Applications in Synthetic Biology

et al. 2018

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Photo-receptors are widely present in both prokaryotic and eukaryotic cells, which serves as the foundation of tuning cell behaviors with light. While practices in eukaryotic cells have been relatively established, trials in bacterial cells have only been emerging in the past few years. A number of light sensors have been engineered in bacteria cells and most of them fall into the categories of two-component and one-component systems. Such a sensor toolbox has enabled practices in controlling synthetic circuits at the level of transcription and protein activity which is a major topic in synthetic biology, according to the central dogma. Additionally, engineered light sensors and practices of tuning synthetic circuits have served as a foundation for achieving light based real-time feedback control. Here, we review programming bacteria cells with light, introducing engineered light sensors in bacteria and their applications, including tuning synthetic circuits and achieving feedback controls over microbial cell culture.

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Section: Light In Tuning Synthetic Circuitsmentioning

confidence: 99%

Section: Light In Tuning Synthetic Circuitsmentioning

confidence: 99%

Section: Light In Tuning Synthetic Circuitsmentioning

confidence: 99%

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Programming Bacteria With Light—Sensors and Applications in Synthetic Biology

et al. 2018

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“…Gene therapy (Collins and Thrasher, 2015 ) for example has been attempted long before optogenetics was even conceived. Direct insertion of functional proteins (Lin et al, 2017 ) or optic control of these proteins (Brechun et al, 2016 ) have also been suggested. The observation of Parkinson symptoms improvement after a localized brain lesion (Dubois et al, 1986 ) inspired the use of stereotactic brain surgery in patients.…”

Section: A Multidisciplinary Contextmentioning

confidence: 99%

And Then There Was Light: Perspectives of Optogenetics for Deep Brain Stimulation and Neuromodulation

et al. 2017

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Deep Brain Stimulation (DBS) has evolved into a well-accepted add-on treatment for patients with severe Parkinsons disease as well as for other chronic neurological conditions. The focal action of electrical stimulation can yield better responses and it exposes the patient to fewer side effects compared to pharmaceuticals distributed throughout the body toward the brain. On the other hand, the current practice of DBS is hampered by the relatively coarse level of neuromodulation achieved. Optogenetics, in contrast, offers the perspective of much more selective actions on the various physiological structures, provided that the stimulated cells are rendered sensitive to the action of light. Optogenetics has experienced tremendous progress since its first in vivo applications about 10 years ago. Recent advancements of viral vector technology for gene transfer substantially reduce vector-associated cytotoxicity and immune responses. This brings about the possibility to transfer this technology into the clinic as a possible alternative to DBS and neuromodulation. New paths could be opened toward a rich panel of clinical applications. Some technical issues still limit the long term use in humans but realistic perspectives quickly emerge. Despite a rapid accumulation of observations about patho-physiological mechanisms, it is still mostly serendipity and empiric adjustments that dictate clinical practice while more efficient logically designed interventions remain rather exceptional. Interestingly, it is also very much the neuro technology developed around optogenetics that offers the most promising tools to fill in the existing knowledge gaps about brain function in health and disease. The present review examines Parkinson's disease and refractory epilepsy as use cases for possible optogenetic stimulation therapies.

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“…Optogenetics has revolutionized research in neuroscience, cell biology and developmental biology by allowing the 'remote control' of cell and animal behaviour with extraordinary precision (1)(2)(3)(4)(5). This precision is achieved by utilizing light as a stimulus that offers unique advantages over pharmacological and genetic manipulation strategies.…”

Section: Introductionmentioning

confidence: 99%

Engineering strategy and vector library for the rapid generation of modular light-controlled protein-protein interactions

Tichy

Gerrard

Legrand

et al. 2019

Preprint

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Optogenetics enables the spatio-temporally precise control of cell and animal behaviour. Many optogenetic tools are driven by light-controlled protein-proteininteractions (PPIs) that are repurposed from natural light-sensitive domains (LSDs).Applying light-controlled PPI to new target proteins is challenging because it is difficult to predict whether one the many available LSDs will yield robust light regulation. As a consequence, fusion protein libraries need to be prepared and tested, but methods and platforms to facilitate this process are currently not available. Here, we developed a genetic engineering strategy and vector library for the rapid generation of lightcontrolled PPIs. The strategy permits fusing a target protein to LSDs efficiently and in two orientations. The public and expandable library contains 29 vectors with blue, green or red light-responsive LSDs many of which have been previously applied ex vivo and in vivo. We demonstrate the versatility of the approach and the necessity for sampling LSDs by generating light-activated caspase-9 (casp9) enzymes.Collectively, this work provides a new resource for optical regulation of a broad range of target proteins in cell and developmental biology.

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Strategies for the photo-control of endogenous protein activity

Cited by 16 publications

References 42 publications

Programming Bacteria With Light—Sensors and Applications in Synthetic Biology

Programming Bacteria With Light—Sensors and Applications in Synthetic Biology

And Then There Was Light: Perspectives of Optogenetics for Deep Brain Stimulation and Neuromodulation

Engineering strategy and vector library for the rapid generation of modular light-controlled protein-protein interactions

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