Strategies for the transformation of filamentous fungi

Ruiz‐Díez, Beatriz

doi:10.1046/j.1365-2672.2002.01516.x

Cited by 185 publications

(114 citation statements)

References 53 publications

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“…Homologous recombination has been the major approach for producing knockout strains in T. reesei with varying levels of success (reviewed by Ruiz-Díez, 2002;Meyer, 2008). Recent advances in method development have resulted in high-throughput generation of knockout/gene replacement strains by more efficient strategies, increasing the efficiency of homologous recombination and allowing sequential gene deletions.…”

Section: Rut-c30 As a Host For Heterologous Protein Productionmentioning

confidence: 99%

Trichoderma reesei RUT-C30 – thirty years of strain improvement

2012

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The hypersecreting mutant Trichoderma reesei RUT-C30 (ATCC 56765) is one of the most widely used strains of filamentous fungi for the production of cellulolytic enzymes and recombinant proteins, and for academic research. The strain was obtained after three rounds of random mutagenesis of the wild-type QM6a in a screening program focused on high cellulase production and catabolite derepression. Whereas RUT-C30 achieves outstanding levels of protein secretion and high cellulolytic activity in comparison to the wild-type QM6a, recombinant protein production has been less successful. Here, we bring together and discuss the results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic-and proteomic-based research on the RUT-C30 strain published over the last 30 years.

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Section: Rut-c30 As a Host For Heterologous Protein Productionmentioning

confidence: 99%

Trichoderma reesei RUT-C30 – thirty years of strain improvement

2012

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“…In recent years, efficient transformation systems have been developed for a wide range of filamentous fungi (reviewed in [1,4,5]). Successful transformation of fungi has been achieved by: CaCl 2 /polyethylene glycol [6][7][8], electroporation [9-15], particle bombardment [2,6,10,16,17], and Agrobacterium tumefaciens-mediated transformation (AMT) [1].…”

Section: Achieving Stable Homokaryotic Transformationmentioning

confidence: 99%

“…However, for random mutagenesis strategies which seek to find unknown genes through phenotypic changes, the presence of untransformed nuclei or nuclei with different integration loci, may prove more challenging. [5,[41][42][43][44]. An alternative to drug resistance genes for transformation of fungi is to use auxotrophic markers such as pyrG (a homologue of the Saccharomyces cerevisiae ura3 gene).…”

Section: Achieving Stable Homokaryotic Transformationmentioning

confidence: 99%

“…While our review covers some of the material in those papers, we have sought to give a broader, comparative overview of the approaches to functional genomics in filamentous fungi and to indicate the directions in which this research is heading. Other general reviews are also available [4,5] and this review updates that work with recent advances. …”

Section: Introductionmentioning

confidence: 99%

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Approaches to functional genomics in filamentous fungi

et al. 2006

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The study of gene function in filamentous fungi is a field of research that has made great advances in very recent years. A number of transformation and gene manipulation strategies have been developed and applied to a diverse and rapidly expanding list of economically important filamentous fungi and oomycetes. With the significant number of fungal genomes now sequenced or being sequenced, functional genomics promises to uncover a great deal of new information in coming years. This review discusses recent advances that have been made in examining gene function in filamentous fungi and describes the advantages and limitations of the different approaches.

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“…Agrobacterium tumefaciens, a plant pathogenic bacterium, transfers part of its Ti plasmid (T-DNA) to the host cell and then inserts the T-DNA region into host genome. 19,20) Using this property, Agrobacterium tumefaciens-mediated transformation (ATMT) is widely applied as a transformation method for plants. It can be used to achieve targeted gene disruption and random insertional mutation.…”

mentioning

confidence: 99%

Construction of an Engineering Strain Producing High Yields of α-TransglucosidaseviaAgrobacterium tumefaciens-Mediated Transformation ofAsperillus niger

Zhou

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Strategies for the transformation of filamentous fungi

Cited by 185 publications

References 53 publications

Trichoderma reesei RUT-C30 – thirty years of strain improvement

Trichoderma reesei RUT-C30 – thirty years of strain improvement

Approaches to functional genomics in filamentous fungi

Construction of an Engineering Strain Producing High Yields of α-TransglucosidaseviaAgrobacterium tumefaciens-Mediated Transformation ofAsperillus niger

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