Strategies, Opportunities, and Challenges in Crop Genetic Diversity Conservation: A Plant Breeder’s Perspective

Singh, Ankita; Behera, Chandana

doi:10.1007/978-981-16-6005-4_7

Cited by 6 publications

(5 citation statements)

References 58 publications

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“…On the other hand, the RADseq technology generates the sequence reads for only the genome-wide restriction sites and such genetic resources enhance understanding of the level of genetic diversity in the sesame population. Even though the higher genome assembly spanning rate was reported for sdRAD-seq data, the ddRAD-seq has predicted more SNPs (27,604) compared to fewer SNPs (13,136) from sdRAD-seq data. This is expected as the ddRAD-seq dataset includes more restriction sites and is expected to be more polymorphic restriction sites than sdRAD-seq [27,28].…”

Section: Plos Onementioning

confidence: 80%

“…Evaluating the genetic diversity of sesame accessions will provide information about how best to use sesame germplasm in a breeding program to accelerate crop improvement. Single nucleotide polymorphism (SNPs) as a molecular marker analysis is one of the most useful methods of investigating the genetic diversity of crop plants [13]. An effective core collection that can capture the maximum genetic diversity of germplasm using the SNP markers would efficiently reduce the number of accessions for phenotypic assessment with only the core collection [14].…”

Section: Introductionmentioning

confidence: 99%

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A pilot-scale comparison between single and double-digest RAD markers generated using GBS strategy in sesame (Sesamum indicum L.)

et al. 2023

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To reduce the genome sequence representation, restriction site-associated DNA sequencing (RAD-seq) protocols is being widely used either with single-digest or double-digest methods. In this study, we genotyped the sesame population (48 sample size) in a pilot scale to compare single and double-digest RAD-seq (sd and ddRAD-seq) methods. We analysed the resulting short-read data generated from both protocols and assessed their performance impacting the downstream analysis using various parameters. The distinct k-mer count and gene presence absence variation (PAV) showed a significant difference between the sesame samples studied. Additionally, the variant calling from both datasets (sdRAD-seq and ddRAD-seq) exhibits a significant difference between them. The combined variants from both datasets helped in identifying the most diverse samples and possible sub-groups in the sesame population. The most diverse samples identified from each analysis (k-mer, gene PAV, SNP count, Heterozygosity, NJ and PCA) can possibly be representative samples holding major diversity of the small sesame population used in this study. The best possible strategies with suggested inputs for modifications to utilize the RAD-seq strategy efficiently on a large dataset containing thousands of samples to be subjected to molecular analysis like diversity, population structure and core development studies were discussed.

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Section: Plos Onementioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

A pilot-scale comparison between single and double-digest RAD markers generated using GBS strategy in sesame (Sesamum indicum L.)

et al. 2023

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“…The confirmation of the identification of the candidate genes and alleles involved with increased Pb tolerance paved the way for targeted breeding programs and genetic engineering approaches aimed at developing Pb-resistant crop varieties. 168 , 170 …”

Section: Genetic and Epigenetic Regulation Of Pb Responses In Plantsmentioning

confidence: 99%

Lead toxicity in plants: mechanistic insights into toxicity, physiological responses of plants and mitigation strategies

Gupta,

Dwivedi,

Kumar

et al. 2024

Plant Signaling & Behavior

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“…Additionally, implementing advanced harvesting methods and post-harvest practices to preserve the quality and freshness of produce incurs expenses related to equipment, training, and infrastructure. Balancing these expenses against the potential benefits of enhanced crop resilience, yield stability, and nutritional quality poses a significant financial challenge for stakeholders along the agricultural value chain [31]. Despite these hurdles, strategic investments and collaborative efforts are essential to overcome these challenges and unlock the full potential of gene-based advancements in agriculture.…”

Section: Upgrading Of Nutritional and Flavor Valuesmentioning

confidence: 99%

Gene-Based Developments in Improving Quality of Tomato: Focus on Firmness, Shelf Life, and Pre- and Post-Harvest Stress Adaptations

Nie,

Yang,

Zheng

et al. 2024

Horticulturae

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Tomato (Solanum lycopersicum) is a widely consumed vegetable crop with significant economic and nutritional importance. This review paper discusses the recent advancements in gene-based approaches to enhance the quality of tomatoes, particularly focusing on firmness, shelf life, and adaptations to pre- and post-harvest stresses. Utilizing genetic engineering techniques, such as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins 9 (CRISPR/Cas9) and Transcription Activator-like Effector Nucleases (TALENs), researchers have made remarkable progress in developing tomatoes with improved traits that address key challenges faced during cultivation, storage, and transportation. We further highlighted the potential of genetic modifications in enhancing tomato firmness, thereby reducing post-harvest losses and improving consumer satisfaction. Furthermore, strategies to extend tomato shelf life through genetic interventions are discussed, emphasizing the importance of maintaining quality and freshness for sustainable food supply chains. Furthermore, the review delves into the ways in which gene-based adaptations can bolster tomatoes against environmental stresses, pests, and diseases, thereby enhancing crop resilience and ensuring stable yields. Emphasizing these crucial facets, this review highlights the essential contribution of genetic advancements in transforming tomato production, elevating quality standards, and promoting the sustainability of tomato cultivation practices.

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Strategies, Opportunities, and Challenges in Crop Genetic Diversity Conservation: A Plant Breeder’s Perspective

Cited by 6 publications

References 58 publications

A pilot-scale comparison between single and double-digest RAD markers generated using GBS strategy in sesame (Sesamum indicum L.)

A pilot-scale comparison between single and double-digest RAD markers generated using GBS strategy in sesame (Sesamum indicum L.)

Lead toxicity in plants: mechanistic insights into toxicity, physiological responses of plants and mitigation strategies

Gene-Based Developments in Improving Quality of Tomato: Focus on Firmness, Shelf Life, and Pre- and Post-Harvest Stress Adaptations

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