Strawberries (Fragaria spp. and Hybrids)

Jungnickel, F.

doi:10.1007/978-3-642-73520-2_2

Cited by 9 publications

(6 citation statements)

References 128 publications

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“…Before the establishment of in vitro culture, the seeds without preliminary treatment were disinfected using 70 % ethanol for 3 min, followed by 3 washes with sterile distilled water. JG-B (Jungnickel/Gliemeroth Basal) medium (Jungnickel, 1988), pH 4.4-4.6 was used to inoculate the seeds of two Ramonda plants.…”

Section: Micropropagation Methodsmentioning

confidence: 99%

“…Plantlets from stage 1 were transferred in three different nutrient media: a) JG-B (Jungnickel/ Gliemeroth Basal) medium (Jungnickel 1988) with phytohormones cytokinin 6-benzylaminopurine (BAP) 0.5 mg l −1 and auxin indole-3-acetic acid (IAA) 0.5 mg l −1 ; b) WPM medium (Lloyd and McCown 1980) with BAP 0.1 and IAA 0.1 mg l −1 ; and c) RA medium (40 % MS) (Tóth et al 2004) with BAP 0.1 and IAA 0.1 mg l −1 . After a month, the developed plantlets were transferred to fresh media for further multiplication.…”

Section: Stage 1 (Seed Germination)mentioning

confidence: 99%

“…Plantlets from previous stage for each species and populations were developed in the three different nutrient media: a) JG-B (Jungnickel/Gliemeroth Basal) medium (Jungnickel 1988) with phytohormones BAP 0.5 and IAA 0.5 mg l −1 ; b) WPM medium (Lloyd and McCown 1980) with BAP 0.1 and IAA 0.1 mg l −1 ; and c) RA medium (40 % MS) (Tóth et al 2004) with BAP 0.1 and IAA 0.1 mg l −1 . In this stage of micropropagation, some differences were observed between plantlets from different populations and nutrient media.…”

Section: Stage 2 (Seedling Development)mentioning

confidence: 99%

“…This procedure is decisive to produce a greater number of plants during a period of 4 months. (Jungnickel, 1988) with phytohormones cytokinin 6-benzylaminopurine (BAP) 0.5 mg l −1 and auxin indole-3-acetic acid (IAA) 0.5 mg l −1 ; WPM medium (Lloyd and McCown, 1980) with BAP 0.1 mg l −1 and IAA 0.1 mg l −1 ; and RA medium (40 % MS) (Tóth et al, 2004) with BAP 0.1 mg l −1 and IAA 0.1 mg l −1 . Mean in each column followed by same letters are not significantly different at the P 0.05 by one-way ANOVA with Duncan's multiple range tests …”

Section: Stage 2 (Seedling Development)mentioning

confidence: 99%

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Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Gashi

Abdullai

Sota

et al. 2014

Physiol Mol Biol Plants

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Ramonda serbica and Ramonda nathaliae are rare and endemo relict plant species from Balkan Peninsula. An efficient micro propagation and in vitro conservation method via direct and indirect organogenesis from seed and leaf explants, respectively, was established in this study. The seed of both Ramonda species were collected from different populations in Kosovo, and were germinated in nutrient media JG-B without any phytohormone. The highest number of shoots and multiplication rate was observed on JG-B medium supplemented with BAP and IAA (0.5 mg l −1 each), whereas the highest number of leaves per plantlets was found on WPM and RA medium supplemented with BAP and IAA (0.1 mg l −1 each). During this stage of micro propagation some significant differences were observed in plantlets from different populations. The indirect organogenesis from parts of leaves of natural plants was not successful due to unavailability of established protocol for disinfections of the plant material. On other hand, parts of leaves from micro propagated plantlets, cultured on MS medium supplemented with different ratio of BAP and NAA, resulted in the highest efficiency for shoot regeneration. In vitro conservation of micro propagated plants at the lower temperature (4°C) had a significantly positive effect for storage of more than 12 months.

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Section: Micropropagation Methodsmentioning

confidence: 99%

Section: Stage 1 (Seed Germination)mentioning

confidence: 99%

Section: Stage 2 (Seedling Development)mentioning

confidence: 99%

Section: Stage 2 (Seedling Development)mentioning

confidence: 99%

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Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Gashi

Abdullai

Sota

et al. 2014

Physiol Mol Biol Plants

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“…Storage of shoot cultures on filter paper bridges in liquid medium at 4 °C in the dark with additional medium added at 3-month intervals is successful for many commercial strawberry genotypes (Mullin and Schlegel, 1976). Strawberry cultures are stored for many years on a semisolid basal medium at low temperature with annual transfers for rejuvenation (Jungnickel, 1988). Cold storage of Fragaria germplasm in semipermeable plastic tissueculture bags ranges from 9-24 months be-Received for publication 22 Mar.…”

Section: Methodsmentioning

confidence: 99%

Photoperiod Improves Long-term Survival of In Vitro-stored Strawberry Plantlets

Reed¹

2002

HortSci

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Cold storage is important for managing in vitro germplasm collections. Strawberry shoot cultures can typically be held at 4 °C for 9 to 24 months before they require repropagation. Concentration of BA in the storage medium, pre-storage cold acclimatization (CA), and exposure to a photoperiod during storage were studied to determine conditions for improved strawberry culture storage. Fragaria shoot cultures stored at 4 °C were rated for plantlet condition on a 0-5 scale at 9, 12, and 19 months. Four species were CA and stored on medium with 0, 1, 2.5, or 5 μm BA either in darkness or under a 12-hour photoperiod. Mean ratings over all treatments and genotypes were best at 9 and 12 months (3.4) and declined at 19 months (2.2). BA in the storage medium significantly improved ratings for two species at 9 and 12 months, but ratings were not significantly different at 19 months. At 19 months of storage, shoot cultures stored with a photoperiod were rated significantly better (P ≤ 0.05) than those grown in darkness. Five Fragaria genotypes stored on medium without BA were used to study the effect of photoperiod and CA on ratings of stored plantlets. CA-shoot cultures stored for 9 or 12 months were rated significantly better than non-CA cultures. After 12 and 19 months storage, three of the five genotypes stored under a 12-hour photoperiod had significantly higher ratings than those stored in the dark (P ≤ 0.01), but by 19 months CA was nonsignificant. Overall, the addition of a photoperiod improved the condition of Fragaria shoot cultures stored at 4 °C. Chemical name used: N6-benzyladenine (BA).

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Regeneration of Plants from Protoplast of Cultivated Strawberry (Fragaria x ananassa) and Wild Strawberry (Fragaria vesca)

Nyman¹,

Wallin

1993

Biotechnology in Agriculture and Forestry

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Strawberries (Fragaria spp. and Hybrids)

Cited by 9 publications

References 128 publications

Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Photoperiod Improves Long-term Survival of In Vitro-stored Strawberry Plantlets

Regeneration of Plants from Protoplast of Cultivated Strawberry (Fragaria x ananassa) and Wild Strawberry (Fragaria vesca)

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