Streptococcins of Indigenous Hemolytic Streptococci

Rasool, Sheikh Ajaz; Ahmed, Samia A.; Iqbal, Alfred

doi:10.1080/10575639608043241

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Five staphylococci isolates (three isolates were selected from our study which are sensitive to most antibiotics (staph4, staph11 and staph31), and two standard strains (obtained from the Central Laboratories: ATCC 12345 and ATCC 98765)) were used as basic indicator strains to determine the most producing staphylococcin isolates, by well diffusion method (Rasool et al, 1996). Nutrient agar plates were inoculated with 100 µL of each basic indicator strains after growing them in a Brain-Heart infusion broth and diluting appropriately to a 0.5 McFarland standard (1.5×108 CFU/ml), then left to dry at room temperature for a period (10-15 minutes).…”

Section: Investigation Of the Efficient Strains Producing Staphylococcinmentioning

confidence: 99%

“…Four staphylococcal isolates (staph19, staph25, staph28 and staph43) were chosen as good Staphylococcin producers according to their widest inhibition zone on the basic indicator isolates. Then the four isolates (producers) were tested against fifty four S. aureus (Indicator) by well diffusion method, as described earlier (Rasool et al, 1996).…”

Section: Typing Of S Aureus Strainsmentioning

confidence: 99%

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Bacteriocin Typing of Staphylococcus Aureus Isolated from Different Sources in Ibb City , Yemen

Al-Gosha’ah¹,

Al-Baker²,

Al-Hetar³

2014

JJBS

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A total of 207 specimens were collected from different sources including patients, health care staff and hospital environment in Ibb city, Yemen. The study used the bacteriocin produced from active producer strains in typing of Staphylococcus aureus. Depending on the morphological, cultural and biochemical characteristics, 54 (26.09%) isolates of Staphylococcus aureus were identified. An antibiotic sensitivity test was done for the bacterial isolates, and the results showed that there were multiple resistant antibiotics. The Staphylococcin production of these isolates has been detected by using wells assay. Fifty one isolates were Staphylococcin producer. Four isolates (staph19, staph25, staph28 and staph43) were chosen as good Staphylococcin producers, and used locally as indicators in bacteriocin typing. Depending on S. aureus typing, the isolates fell into (9) groups. The most numerous group was characterized by susceptibility to all four staphylococcin and comprised 61.11% isolates of S. aureus, while the lowest numerous were found in three groups with a ratio of 1.85%; the remaining groups had little percentages ranging from 3.70% to 11.11%. We observed that about (94.44%) of the isolates were bacteriocin producers, and among them, four isolates had a strong bacteriocin production. Based on typing, most isolates had one pattern.

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Section: Investigation Of the Efficient Strains Producing Staphylococcinmentioning

confidence: 99%

Section: Typing Of S Aureus Strainsmentioning

confidence: 99%

Bacteriocin Typing of Staphylococcus Aureus Isolated from Different Sources in Ibb City , Yemen

Al-Gosha’ah¹,

Al-Baker²,

Al-Hetar³

2014

JJBS

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“…The thermo stability of the bacteriocin preparations were determined by heating 2.0 ml of the preparations at 40 0 C, 60 0 C, 80 0 C and 100 0 C for 30 min. Samples were removed at intervals of 5 minutes, cooled and assayed for remaining activity as done by Rasool et al [13].…”

Section: 1 Effect Of Ph and Temperaturementioning

confidence: 99%

Potential use of bacteriocin producing lactic acid bacterial strain isolated from milk products and its application as the fish feed

Khumbongmayum¹,

Veenagayathri²

2016

Int. J. Sci. World

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Bacteriocins are gene-encoded inhibitory proteins and those produced by Gram-positive Lactic acid bacteria. Some bacteriocins even display antagonistic activity towards Gram-positive food borne pathogens and spoilage organisms. This present study involves isolation of Bacteriocin-producing lactic acid bacteria from a variety of milk and milk products. The physico-chemical properties of the isolated bacteriocin producing bacterial strains were screened. The isolated bacteriocin bacterial strains were biochemically characterized and identified. Further, the isolated effective bacterial strain was used as a fish feed and its effect on their growth was evaluated. The evaluated data continue to demonstrate that the bacteriocin producing bacterial strains will have greater potential in the food products industry.

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“…The inhibition against the indicator organism was recorded. The activity of the bacteriocin in the presence of chloroform was performed by mixing the culture supernatant with chloroform (at ratio of 1:1), incubated for 30 min followed by evaporating the remaining solvent before testing against the indicator organism (Rasool et al 1996).…”

Section: Effects Of Temperature Ph Enzymes and Chloroform On Bacterio...mentioning

confidence: 99%

Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus

Yusuf

Hamid

2013

Journal of Agrobiology

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Lactic acid bacteria (LAB) were isolated from the bile, gizzard, intestine and caecum of the Malaysian domestic non-broiler chicken. Of the 56 isolates grown on MRS agar plates, 7 - namely, B3L3, B4L4, G5L5, B5L6, B10L7, I1L8, and C4L10 - showed inhibition against the methicillin resistant Staphylococcus aureus (MRSA). These isolates were all Gram positive cocci, non-spore forming, and catalase negative. The purified fraction from the three phase partitioning (TPP) method, employing t-butanol and ammonium sulfate, produced inhibitory zones of 8 to 18 mm in diameter on the indicator organism, Staphylococcus aureus (MRSA). The TPP purified fraction contained heat stable proteins of about 10 kDa in size and was inactivated by trypsin. Based on the partial rDNA sequences (>89% similarity), isolates B4L4 and G5L5 belonged to Enterococcus hirae; isolate B3L3 belonged to Enterococcus faecium; isolates B5L6, B10L7, I1L8 to Enterococcus faecalis; and isolate C4L10 to Enterococcus mundtii. These probiotic strains, exclusively isolated from the Malaysian non-broiler chicken, produced an antimicrobial protein or bacteriocin which was active against a pathogenic strain of methicillin resistant Staphylococcus aureus (MRSA).

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Streptococcins of Indigenous Hemolytic Streptococci

Cited by 4 publications

References 13 publications

Bacteriocin Typing of Staphylococcus Aureus Isolated from Different Sources in Ibb City , Yemen

Bacteriocin Typing of Staphylococcus Aureus Isolated from Different Sources in Ibb City , Yemen

Potential use of bacteriocin producing lactic acid bacterial strain isolated from milk products and its application as the fish feed

Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus

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