Streptococcocis occurence at rainbow trout (Oncorhynchus mykiss, Walbaum) cultivated in province Mersin-Turkey)

Abstract

Özet:Mersin ilinde faaliyet gösteren yedi Gökkuşağı alabalığı işletmesinde bir yıl süreyle 260 adet farklı büyüklüklerde balık örneği ve 56 adet su örneği Gram pozitif bakteri florası açısından klasik izolasyon ve identifikasyon yöntemleriyle incelenmiştir. İzolatların identifikasyonu ticari ID32Strep hızlı kiti (BioMerieux) ile yapılmıştır. Balık örneklerinin 63'ünde (%24.23) ve su örneklerinin 9'unda (%16.07) 59 adet Gram pozitif, katalaz ve oksidaz negatif kok izole edilmiştir. V. İşletmede yıl boyunca herhangi bir kokkal izolata rastlanmazken, işletmelerin hiçbirinde kış aylarında Gram pozitif, katalaz ve oksidaz negatif kok saptanmamıştır. Gram pozitif, katalaz ve oksidaz negatif kok izolatlar Aerococcus viridans, Enterococcus durans/hirae, Enterococcus faecium-1, Lactococcus garvieae, Lactococcus lactis lactis, Leuconostoc spp. ve Streptococcus anginosus olarak identifiye edilmiştir. Bu bakterilerin izole edildiği 62 balıktan 28'inde (%45.16) renkte kararma, gözde matlık, tek ya da çift taraftlı eksoftalmus, solungaçta kansızlık gibi hastalık belirtileri ve 7'sinde (%11.29) karaciğerde kanama ve anemi görülmüştür. Antibakteriyel duyarlılık testleri bu izolatların gentamisin, oksitetrasiklin, neomisin, enrofloksasin, eritromisin ve streptomisin'e duyarlı olduklarını gösterirken çoğunun sulfametoksazol-trimetoprim'e dirençli olduklarını ortaya koymuştur. MersinÜniversitesi BAP-SÜF-YB (SÖ) 2004-2 no'lu projenin bir kısmıdır. Özer ve ark., 2(3): 272-283 (2008) 273 Abstract: Streptococcocis occurence at rainbow trout ( Oncorhynchus mykiss, Walbaum) cultivated in province Mersin-TurkeyDuring a one year period 260 various sized fish samples and 56 water samples at seven rainbow trout farms in province Mersin were investigated for Gram positive-catalase and oxydase negative coccal bacteria with classical isolation and identification methods. The isolates were identified with ID32Strep rapid kit. 59 Gram positive-catalase and oxydase negative cocci were isolated from 63 (24.23%) fish samples and 9 (16.07%) water samples. Gram positive-catalase and oxydase negative cocci were not found at fish farms in winter and at the fifth farm during the year. Gram positive-catalase negative coccal isolates were identified as Aerococcus viridans, Enterococcus durans/hirae, Enterococcus faecium-1, Lactococcus garvieae, Lactococcus lactis lactis, Leuconostoc spp. and Streptococcus anginosus. Clinical signs like darkening of skin, corneal opacity, uni-or bilateral exophthalmia, anemic gills were diagnosed at 28 of 62 bacteria-isolated fishes (45.16%) and 7 (11.29%) of them had haemorraghia and anemia at liver. While antibacterial susceptibility tests verified, that these isolates were susceptible to gentamycin, oxitetracycline, neomycin, enrofloxacin, eritromycin and streptomycin, most of them were resistant to sulphamethoxazole-trimetoprim.

Synthesis and characterization of N -(alky(aryl)carbamothioyl)cyclohexanecarboxamide derivatives and their Ni(II) and Cu(II) complexes

“…Acetone was dried by shaking with anhydrous calcium sulfate (Drierite) for several hours, followed by careful fractional distillation. The ligands were prepared by a procedure similar to that reported in the literature [8,32]. A solution of cyclohexanecarbonyl chloride (0.005 mol) in acetone (30 cm 3 ) was added dropwise to a suspension of potassium thiocyanate (0.005 mol) in acetone (30 cm 3 ).…”

“…Metallic complexes were prepared according to the method described[8,32].A solution of metallic acetate (0.01 mol) in ethanol (30 cm 3 ) was added dropwise to a solution of the ligand in a 1 : 2 ratio for all metal with a small excess of ligand in ethanol (30 cm 3 ) at room temperature, and the resulting mixture was stirred for 30 min. The solid complexes were filtered and recrystallized from an ethanol : dichloromethane mixture (1 : 2).…”

Crystal structure of cis-copper(II) complex with N-(di-n-propylcarbamothioyl)cyclohexanecarboxamide ligand

“…The copper(II) complex was prepared according to the method described [25][26][27]. The solution of ligand (10.0 mmol) in ethanol (50 mL) was added dropwise a solution of copper(II) acetate (10.0 mmol) in etanol (50 mL) at room temperature.…”

Parasite Fauna of Fish in Büyükçekmece Dam Lake

“…(Ehrenberg, 1831) Trichodina is a group of dorsoventrally flattened oval ciliated protozoan parasites that lives in freshwater, brackish water and sea water. Several Trichodina species were identified in different environments in Turkey Erdem, 1998, 1999;Özer at al., 2010;Öğüt and Altuntaş, 2011). Although organic pollution gradient was becoming to increase Trichodina sp., Trichodina spp.…”