Streptococcus gallolyticus abrogates anti-carcinogenic properties of tannic acid on low-passage colorectal carcinomas

Abstract

the tannase-producing Gram-positive bacterial species Streptococcus gallolyticus subsp. gallolyticus (Sgg) is an opportunistic pathogen of the human gut and strongly associated with colorectal cancer (cRc). A unique feature of Sgg is its ability to degrade tannic acids (tA). tA constitute an important part of the human diet with known anti-tumorigenic properties. Here, we examined whether Sgg is able to protect tumor cells from the toxic effect of TA and thus drive tumorigenesis indirectly. Human CRC cell lines (n = 8) were treated with increasing concentrations of TA. We confirmed the cytotoxic activity of TA in a dose-dependent manner. In virtually all cell lines, viability decreased significantly (>60% inhibition). Moreover, pyrogallol, the degradation product of TA, had no effect on the tested cell lines. This suggests a specific effect of TA. Cytotoxicity was due to necrosis and induction of senescence in residual cells. finally, when tA was degraded by Sgg, the cytotoxic effect could be abolished. Tumor cells even responded with boosted cell proliferation, highlighting the impact of Sgg on cRc progression. We here provide another piece of evidence for the active interplay between Sgg and cancer preventive components. these data will help to move forward in designing concepts for therapeutic and eventually also prophylactic approaches to combat gastrointestinal malignancies.

A structurally unique Fusobacterium nucleatum tannase provides detoxicant activity against gallotannins and pathogen resistance

“…However, and despite the importance of the diet in the aetiology of colorectal malignancies, no information is available regarding the genetic determinants that regulate the interaction of the bacterium with food components in order to facilitate their survival and growth within the tumour environment. Recently, tannic acids have been demonstrated to be cytotoxic for a panel of CRC‐derived cells lines, while their degradative product, pyrogallol, did not affect the growth of these cells (Oehmcke‐Hecht et al ., 2020 ). Indeed, the presence of the opportunistic gut pathogen, Streptococcus gallolyticus , abrogated the cytotoxic capacity of tannic acids (Oehmcke‐Hecht et al ., 2020 ).…”

“…Recently, tannic acids have been demonstrated to be cytotoxic for a panel of CRC‐derived cells lines, while their degradative product, pyrogallol, did not affect the growth of these cells (Oehmcke‐Hecht et al ., 2020 ). Indeed, the presence of the opportunistic gut pathogen, Streptococcus gallolyticus , abrogated the cytotoxic capacity of tannic acids (Oehmcke‐Hecht et al ., 2020 ). Increasing evidence indicates that oral pathobionts found in the gut during disorders such as CRC seem to originate from the oral niche through translocation mechanisms to the gastrointestinal tract that are currently unknown (Flemer et al ., 2018 ; Schmidt et al ., 2019 ).…”

Gut microbiota, inflammatory bowel disease and colorectal cancer

“…There is plenty of evidence of the tumor-promoting effects of Streptococcus gallolyticus in colon cells[ 35 - 37 ]. Colonic cells incubated with Streptococcus gallolyticus ( S. gallolyticus ) presented increased levels of β-catenin, c-Myc, and proliferating cell nuclear antigen (PCNA), all of which are transcription factors associated with cancer development[ 35 ].…”

Understanding the Role of the Microbiome in Cancer Diagnostics and Therapeutics by Creating and Utilizing ML Models

“…Promoters of antitumor response by TLR4, TNF production, although prescription of antibiotics may alter. [19,44] Oscillospiraceae Microbiota composition, antibiotics before ipilimumab treatment does not influence baseline dominant microbiota. [11,44] Table 7.…”

“…[19,44] Oscillospiraceae Microbiota composition, antibiotics before ipilimumab treatment does not influence baseline dominant microbiota. [11,44] Table 7. Cont.…”