Streptococcus gordonii Poised for Glycan Feeding through a MUC5B-Discriminating, Lipoteichoic Acid-Mediated Outside-In Signaling Circuit

Lima, Bruno P.; Davies, Julia R.; Wickström, Claes; Johnstone, Karen F.; Hall, Jeffrey W.; Svensäter, Gunnel; Herzberg, Mark C.

doi:10.1128/jb.00118-22

Cited by 5 publications

(3 citation statements)

References 69 publications

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“…In addition, MUC1 also binds to bacteria, including Pseudomonas aeruginosa [77], and Helicobacter pylori [78]. Furthermore, mucins could serve as a source of carbohydrates for commensal species, such as Streptococcus gordonii catabolism [79] or as decoys for clearance of cariogenic Streptococcus mutans [80,81], indicating a role in the maintenance and homeostasis of the oral microenvironment. Since both DFK and KSFM support the expression and release of mucins in OKF6/TERT-2 cells, similar to in vivo oral epithelial cells, either media could be used for further studies in mucin expression and function.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Culture Media for in-vitro Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Cuadra¹,

Shamim²,

Shah³

et al. 2023

Preprint

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Background: Understanding in vitro expansion of OKF6/TERT-2 oral epithelial cells is important for studying molecular biology of disease and pathology affecting the oral cavity. The media used for any cell culture is paramount in terms of efficient output. Therefore, this study aims to compare two different media for OKF6/TERT-2 cultures: Keratinocyte Serum-Free Medium (KSFM) and a composite medium comprised of DMEM/F-12 mixed with KSFM (referred to as DFK). For application purposes, this investigation also compares the toxicological effects of flavored electronic cigarette liquids (E-liquids) on OKF6/TERT-2 cultures grown in both media. Methods: Cells were grown in KSFM and DFK media and cellular growth, morphology, gene expression of mucins and tight junctions, as well as wound-healing were determined. Additionally, cellular viability and cytotoxicity were indexed after E-liquid exposures. Results: While overall cellular morphologies remained unaltered, cells grown in DFK reached confluency faster. Except for claudin-1, there is no appreciable difference in expression of the other genes tested. Additionally, cultures in DFK appear more sensitive to E-liquids ± flavors. Conclusions: DFK is an alternative medium for cultivation of OKF6/TERT-2 cells to study molecular biology of disease and pathology, such as their responses to E-liquids ± flavors.

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Section: Discussionmentioning

confidence: 99%

Comparison of Culture Media for in-vitro Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Cuadra¹,

Shamim²,

Shah³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition, MUC1 also binds to bacteria, including Pseudomonas aeruginosa [80], and Helicobacter pylori [81]. Furthermore, mucins could serve as a source of carbohydrates for commensal species, such as Streptococcus gordonii catabolism [82] or as decoys for clearance of cariogenic Streptococcus mutans [83,84], indicating a role in the maintenance and homeostasis of the oral microenvironment. Since both DFK and KSFM support the expression and release of mucins in OKF6/TERT-2 cells, similar to in vivo oral epithelial cells, either media could be used for further studies in mucin expression and function.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Culture Media for in-vitro Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Cuadra¹,

Shamim²,

Shah³

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Expansion of OKF6/TERT-2 oral epithelial cells in vitro is important for studying the molecular biology of disease and pathology affecting the oral cavity. Keratinocyte Serum-Free Medium (KSFM) is the medium of choice for this cell line. This study compares three media for OKF6/TERT-2 cultures: KSFM, Dulbecco’s Modified Eagle Medium/Nutrient Mixture of Hams F-12 (DMEM/F12) and a composite medium comprised of DMEM/F-12 and KSFM (1:1 v/v), referred as DFK. The toxicological effects of electronic cigarette liquids (E-liquids) on OKF6/TERT-2 cells cultured in these media were also compared. Methods: Cells were cultured in KSFM, DMEM/F12 or DFK and cellular morphology, growth, wound healing and gene expression of mucins and tight junctions were evaluated. Additionally, cytotoxicity was determined after E-liquid exposures. Results: Switching from KSFM to DMEM/F12 or DFK 24-hours post-seeding leads to typical cellular morphologies, and these cultures reach confluency faster than those in KSFM. Wound-healing recovery occurred fastest in DFK. Except for claudin-1, there is no difference in expression of the other genes tested. Additionally, E-liquid cytotoxicity appears to be amplified in DFK cultures. Conclusions: DMEM/F12 and DFK are alternative media for OKF6/TERT-2 cell culture to study molecular biology of disease and pathology, provided cells are initially seeded in KSFM.

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“…For example, MUC1 binds to adenovirus, reducing infection into host cells [80], where the virus binds to O-linked carbohydrates on the mucin [81]. In addition, MUC1 also binds to non-oral bacteria [82,83], as well as oral species such as Streptococcus gordonii [84] or Streptococcus mutans [54,85], implicating a role in the maintenance and homeostasis of the oral microenvironment. Since both DFK and KSFM support the expression and release of mucins in OKF6/TERT-2 cells, similar to in vivo oral epithelial cells, this further suggests that either medium could be used for studies investigating mucin expression and function.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Culture Media for In Vitro Expansion of Oral Epithelial Keratinocytes

Cuadra

Shamim

Shah

et al. 2023

Applied Biosciences

View full text Add to dashboard Cite

Background: Expansion of OKF6/TERT-2 oral epithelial cells in vitro is important for studying the molecular biology of disease and pathology affecting the oral cavity. Keratinocyte serum-free medium (KSFM) is the medium of choice for this cell line. This study compares three media for OKF6/TERT-2 cultures: KSFM, Dulbecco’s Modified Eagle Medium/Nutrient Mixture of Hams F-12 (DMEM/F12), and a composite medium comprised of DMEM/F-12 and KSFM (1:1 v/v), referred to as DFK. The toxicological effects of electronic cigarette liquids (e-liquids) on OKF6/TERT-2 cells cultured in these media were also compared. Methods: Cells were cultured in KSFM, DMEM/F12, or DFK, and cellular morphology, growth, wound healing and the gene expression of mucins and tight junctions were evaluated. Additionally, cytotoxicity was determined after e-liquid exposures. Results: Switching from KSFM to DMEM/F12 or DFK 24 h post-seeding leads to typical cellular morphologies, and these cultures reach confluency faster than those in KSFM. Wound-healing recovery occurred fastest in DFK. Except for claudin-1, there is no difference in expression of the other genes tested. Additionally, e-liquid cytotoxicity appears to be amplified in DFK cultures. Conclusions: DMEM/F12 and DFK are alternative media for OKF6/TERT-2 cell culture to study the molecular biology of disease and pathology, provided cells are initially seeded in KSFM.

show abstract

Streptococcus gordonii Poised for Glycan Feeding through a MUC5B-Discriminating, Lipoteichoic Acid-Mediated Outside-In Signaling Circuit

Cited by 5 publications

References 69 publications

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Comparison of Culture Media for In Vitro Expansion of Oral Epithelial Keratinocytes

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