Streptococcus mutans gtfA gene specifies sucrose phosphorylase

Russell, R. R. B.; Mukasa, Hidehiko; Shimamura, Atsunari; Ferretti, Joseph J.

doi:10.1128/iai.56.10.2763-2765.1988

Cited by 44 publications

(19 citation statements)

References 31 publications

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“…Further analysis of the product formed from sucrose by purified enzyme encoded by the ggg gene indicates that the GtfA enzyme is most likely a sucrose phosphorylase, which catalyzes the transfer of a glucosyl moiety from sucrose to Pi to generate a-D-glucose-1-phosphate and free fructose (22). Since both glucan and glucose-i-phosphate have similar mobilities in paper and thin-layer chromatography (21,22), and they are also methanol insoluble, this new finding (22) is consistent with previous reports (19)(20)(21). However, the identity of the reaction products of the GtfA enzyme with sucrose as the substrate has not been fully solved.…”

supporting

confidence: 87%

Impairment of melibiose utilization in Streptococcus mutans serotype c gtfA mutants

Barletta

Curtiss

1989

Infect Immun

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The Streptococcus mutans serotype c gtfA gene encodes a 55-kilodalton sucrose-hydrolyzing enzyme. Analysis of S. mutans gtfA mutants revealed that the mutant strains were specifically impaired in the ability to use melibiose as a sole carbon source. S. mutans gafA mutant strains synthesized less a-galactosidase activity inducible by raffinose than wild-type strains. Melibiose (an inducer in wild-type strains) failed to induce significant levels of a-galactosidase in the mutant strains. We hypothesize that melibiose use by S. mutans requires the interaction of the GtfA enzyme, or another gene product under the control of the gifA promoter, with other gene product(s) involved in melibiose transport or hydrolysis.

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supporting

confidence: 87%

Impairment of melibiose utilization in Streptococcus mutans serotype c gtfA mutants

Barletta

Curtiss

1989

Infect Immun

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“…It was also identified in bacteria from other environments, for example in Leuconostoc mesenteroides (Kagan et al. 1942) or Pseudomonas saccharophila (Doudoroff 1943), as well as in Streptococcus mutans, Bifidobacterium longum, B. adolescentis and B. lactis (Russell et al. 1988; Kim et al.…”

Section: Discussionmentioning

confidence: 99%

Sucrose phosphorylase of the rumen bacteriumPseudobutyrivibrio ruminisstrain A

Kasperowicz

Stan-Glasek

Guczynska

et al. 2009

Journal of Applied Microbiology

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Aims: To verify the taxonomic affiliation of bacterium Butyrivibrio fibrisolvens strain A from our collection and to characterize its enzyme(s) responsible for digestion of sucrose. Methods and Results: Comparison of the 16S rRNA gene of the bacterium with GenBank showed over 99% sequence identity to the species Pseudobutyrivibrio ruminis. Molecular filtration, native electrophoresis on polyacrylamide gel, zymography and thin layer chromatography were used to identify and characterize the relevant enzyme. An intracellular sucrose phosphorylase with an approximate molecular mass of 52 kDa exhibiting maximum activity at pH 6·0 and temperature 45°C was identified. The enzyme was of inducible character and catalysed the reversible conversion of sucrose to fructose and glucose‐1‐P. The reaction required inorganic phosphate. The Km for glucose‐1‐P formation and fructose release were 3·88 × 10−3 and 5·56 × 10−3 mol l−1 sucrose, respectively – while the Vmax of the reactions were −0·579 and 0·9 μmol mg protein−1 min−1. The enzyme also released free glucose from glucose phosphate. Conclusion: Pseudobutyrivibrio ruminis strain A utilized sucrose by phosphorolytic cleavage. Significance and Impact of the Study: Bacterium P. ruminis strain A probably participates in the transfer of energy from dietetary sucrose to the host animal.

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“…SPF Sprague-Dawley rats (Kingston Facility, Charles River Breeding Laboratories) were utilized to test for caries induction as previously described (4). Animals (approximately 21 days old) were divided into groups of 12 animals each. All rats were fed diet 2000 containing 56% sucrose as well as drinking water containing 10% sucrose.…”

Section: Methodsmentioning

confidence: 99%

Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model

et al. 1993

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The role of each of the Streptococcus mutans gif genes coding for glucan synthesis in cariogenesis was evaluated by using strain UA130 in the specific-pathogen-free (SPF) rat model system. Mutants defective in either or both of the genes required for insoluble glucan synthesis, the g#B7 and goT genes, exhibited markedly reduced levels of smooth-surface carious lesions relative to that of the parental organism. Likewise, the mutant defective in the gtJD gene coding for the glucosyltransferase-S enzyme synthesizing water-soluble glucans also produced significantly fewer smooth-surface lesions than strain UA130. None of these mutations markedly altered the rate of sulcal caries induction relative to that of the parental organism. In addition, a mutant of strain UA130 defective in the gtfA gene was reexamined in the SPF rat model. In contrast to previous results from a gnotobiotic rat system, these mutants also induced significantly fewer smooth-surface carious lesions compared with that by strain UA130. These results suggest that all four genes are important for smooth-surface caries formation. Furthermore, these results are discussed relative to the differences in the diets utilized in the SPF and gnotobiotic rat model systems for assessing the virulence factors of S. mutans. Strain Relevant property Source S. mutans UA130S UA130 maintained in San Antonio, Tex. P. Caufielda UA13OS(pTH10) UA130S containing pTH10 (Em') This study UA13OR UA130 maintained in Rochester, N.Y. P. Caufielda GS5B GS5 with inactive gtfB (Emr) 1 GS5DD GS5 with inactive gtfD (Tetr) 9 UA130B UA130S with inactive gtfB (Emr) This study UA130C UA130S with inactive gtfC (Em') This study UA130BC UA130S with inactive gtfB and gtfC (Emr) This study UA13ODD UA130S with inactive gtfD (Tetr) This study UAB747 UA130 with inactive gtfA (Em') 2 UA13ORR UA13OR isolated from SPF rats This study UA130SR UA130(pTH10) isolated from SPF rats (Ems) This study S. sobrinus 6715 Highly cariogenic in rats 4

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Streptococcus mutans gtfA gene specifies sucrose phosphorylase

Cited by 44 publications

References 31 publications

Impairment of melibiose utilization in Streptococcus mutans serotype c gtfA mutants

Impairment of melibiose utilization in Streptococcus mutans serotype c gtfA mutants

Sucrose phosphorylase of the rumen bacteriumPseudobutyrivibrio ruminisstrain A

Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model

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