Streptococcus pneumoniae Colonization Is Required To Alter the Nasal Microbiota in Cigarette Smoke-Exposed Mice

Shen, Pamela; Whelan, Fiona; Schenck, L. Patrick; McGrath, Joshua J.C.; Vanderstocken, Gilles; Bowdish, Dawn M. E.; Surette, Michael G.; Stämpfli, Martin R.

doi:10.1128/iai.00434-17

Cited by 13 publications

(14 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies suggested a direct alteration of bacterial infection and carriage pathways, as it has already been shown that S. aureus invasion and biofilm formation are elevated after cigarette exposure [47, 113, 114]. A similar effect was observed for pneumococcal biofilms [115, 116] (Additional file 1).…”

Section: The Upper Respiratory Tract Microbiome Changes With Age and supporting

confidence: 55%

The microbiome of the upper respiratory tract in health and disease

et al. 2019

View full text Add to dashboard Cite

The human upper respiratory tract (URT) offers a variety of niches for microbial colonization. Local microbial communities are shaped by the different characteristics of the specific location within the URT, but also by the interaction with both external and intrinsic factors, such as ageing, diseases, immune responses, olfactory function, and lifestyle habits such as smoking. We summarize here the current knowledge about the URT microbiome in health and disease, discuss methodological issues, and consider the potential of the nasal microbiome to be used for medical diagnostics and as a target for therapy.

show abstract

Section: The Upper Respiratory Tract Microbiome Changes With Age and supporting

confidence: 55%

The microbiome of the upper respiratory tract in health and disease

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The intestinal microbiota can protect itself against colonization with new bacteria (colonization resistance), while dysbiosis is apparently exploited by CRE for colonization. On the other hand, it is also possible that the established CRE colonization induces significant perturbations to the microbiota, which in turn may act as a pathogenic community to perpetuate host pathology (15).…”

Section: Discussionmentioning

confidence: 99%

Intestinal Dysbiosis in Carriers of Carbapenem-Resistant Enterobacteriaceae

Korach-Rechtman

Hreish

et al. 2020

mSphere

View full text Add to dashboard Cite

Infection with carbapenem-resistant Enterobacteriaceae (CRE) has become an important challenge in health care settings and a growing concern worldwide. Since infection is preceded by colonization, an understanding of the latter may reduce CRE infections. We aimed to characterize the gut microbiota in CRE carriers, assuming that microbiota alterations precede CRE colonization. We evaluated the gut microbiota using 16S rRNA gene sequencing extracted of fecal samples collected from hospitalized CRE carriers and two control groups, hospitalized noncarriers and healthy adults. The microbiota diversity and composition in CRE-colonized patients differed from those of the control group participants. These CRE carriers displayed lower phylogenetic diversity and dysbiotic microbiota, enriched with members of the family Enterobacteriaceae. Concurrent with the enrichment in Enterobacteriaceae, a depletion of anaerobic commensals was observed. Additionally, changes in several predicted metabolic pathways were observed for the CRE carriers. Concomitantly, we found higher prevalence of bacteremia in the CRE carriers. Several clinical factors that might induce changes in the microbiota were examined and found to be insignificant between the groups. The compositional and functional changes in the microbiota of CRE-colonized patients are associated with increased risk for systemic infection. Our study results provide justification for attempts to restore the dysbiotic microbiota with probiotics or fecal transplantation. IMPORTANCE The gut microbiota plays important roles in the host’s normal function and health, including protection against colonization by pathogenic bacteria. Alterations in the gut microbial profile can potentially serve as an early diagnostic tool, as well as a therapeutic strategy against colonization by and carriage of harmful bacteria, including antibiotic-resistant pathogens. Here, we show that the microbiota of hospitalized patients demonstrated specific taxa which differed between carriers of carbapenem-resistant Enterobacteriaceae (CRE) and noncarriers. The difference in the microbiota also dictates alterations in microbiome-specific metabolic capabilities, in association with increased prevalence of systemic infection. Reintroducing specific strains and/or correction of dysbiosis with probiotics or fecal transplantation may potentially lead to colonization by bacterial taxa responsible for protection against or depletion of antibiotic-resistant pathogens.

show abstract

“…Tobacco smoke exposure during pregnancy is one risk factor for GBS colonization that has not yet been adequately explored. Tobacco smoke has been shown to be associated with increased colonization of the respiratory, gastrointestinal, and even genital tracts with various bacterial pathogens [15][16][17][18][19][20][21]. However, the impact of tobacco smoke exposure on GBS colonization has not been adequately explored.…”

Section: Introductionmentioning

confidence: 99%

Group B streptococcal colonization: Prevalence and impact of smoking in women delivering term or near term neonates in a large tertiary care hospital in the southern United States

et al. 2020

View full text Add to dashboard Cite

Background and hypothesis The role of smoking as a risk factor for group B streptococcal (GBS) colonization in women during pregnancy has not been previously adequately explored. We hypothesized that women of term or near term neonates who smoked during pregnancy were more likely to have GBS colonization than their non-smoking counterparts. Methods The electronic health records (EHRs) of a convenience sample of women delivering in an inner-city university tertiary care center were reviewed. The outcome variable of interest was maternal GBS colonization during pregnancy. The primary independent variable of interest was tobacco smoking during pregnancy, determined from the EHRs by the number of cigarettes smoked during gestation. Descriptive statistics were conducted and categorical data were compared by the Fischer's exact test. Multiple logistic regression analysis was further conducted to determine the independent impact of tobacco smoke exposure on GBS colonization. Results The prevalence of maternal GBS colonization was 35% among the study population. In the univariate analyses, factors associated with maternal GBS colonization were tobacco smoking during pregnancy (P of trend <0.001), Race (P<0.001), maternal age <20 years (P = 0.006), low birthweight <2500 gm (P = 0.020), maternal drug use (P = 004), and gestational age <37 (P = 0.041). In a multiple logistic regression analysis, tobacco smoking during pregnancy remained the most significant predictor of GBS colonization. Women who smoked during pregnancy were more than twice more likely to be colonized than their non-smoking counterparts (OR = 2.6; 95% CI = 1.5-4.6; p<0.001). Maternal age was the only other

show abstract

Streptococcus pneumoniae Colonization Is Required To Alter the Nasal Microbiota in Cigarette Smoke-Exposed Mice

Cited by 13 publications

References 50 publications

The microbiome of the upper respiratory tract in health and disease

The microbiome of the upper respiratory tract in health and disease

Intestinal Dysbiosis in Carriers of Carbapenem-Resistant Enterobacteriaceae

Group B streptococcal colonization: Prevalence and impact of smoking in women delivering term or near term neonates in a large tertiary care hospital in the southern United States

Contact Info

Product

Resources

About