Streptokinase Loading Fabrication Magnetic Nanoparticle Supported With Tannic Acid as a Modified Thrombolytic Agent

Abed, Hayder H.; Al-Wasiti, Estabraq A.; Tawfeeq, Amir T.

doi:10.36295/asro.2019.22125

Cited by 4 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Blood samples (2ml each) were collected from human volunteers (N=202) with diabetes mellitus type 2. This was centrifuged at 1600rpm for 10 minutes and the serum was transferred into a new tube and used to determine the level of serum vitamin B12 (Abed et al, 2019;Sugihara et al, 2017).…”

Section: Blood Sample Collectionmentioning

confidence: 99%

Untitled

2023

Pak. J. Pharm. Sci.,

View full text Add to dashboard Cite

Diabetes mellitus type 2 is characterized by insulin resistance, which can be combined with relatively decrease secretion of insulin hormone in the body. Metformin is usually recommended as a first-line treatment for diabetes mellitus-type 2, as it has a significant role in decrease mortality. This study aims to evaluate the B12 levels in Iraqi patients with type 2 diabetes, who were using the metformin drug in short-, medium-and long-term periods. The study included 202 patients, who were classified into four groups, according to their use of the Metformin drug as a hypoglycemic agent, while the fourth group did not use metformin and was termed as 'the standard group'. The results showed that the levels of vitamin B12 in patients using Metformin drugs in short-, medium-and long-term periods were, (444.4±17.21)pg/ml, (403.0±20.34)pg/ml and (261.7±14.70)pg/ml, respectively, compared to the standard group (469.7±26.37)pg/ml. The conclusion of this study was that a significant reduction was seen in the level of vitamin B12 in patients using the Metformin drug for medium-and long-term periods compared to the standard group, who did not use the Metformin drug.

show abstract

Section: Blood Sample Collectionmentioning

confidence: 99%

Untitled

2023

Pak. J. Pharm. Sci.,

View full text Add to dashboard Cite

show abstract

“…Blood was collected immediately after semen collection and transferred into a plain tube. This was left to stand for 10 minutes to allow the blood to clot, then centrifuged at 1,600 rpm for 10 minutes (Abed et al 2019). The serum was transferred into a new tube and kept in freezer until use.…”

Section: Blood Collectionmentioning

confidence: 99%

Effect of Vitamin D3 Supplement on the Semen Quality in Human Patients with Vitamin D Deficiency

Abed¹,

Ali²,

Mahdi³

2022

HAYATI J Biosci

Self Cite

View full text Add to dashboard Cite

Thermophilic bacteria are a source of bioactive compounds that have many benefits for human life. One of them is as a source of antimicrobials. This research aimed to identify and characterize the promising thermophilic bacterial isolates by analyzing bioactive compounds and their potential as antimicrobial agents. Thermophilic bacteria with the code LBKURCC were taken from the collection of the Biochemistry Laboratory of the University of Riau. Forty-four purified strains of thermophilic bacteria were tested for antimicrobial ability. These thermophilic bacteria were taken from hot springs located in the Sumatra provinces of West Sumatra and Riau. Strain LBKURCC218 isolated from Rimbo Panti hot springs in West Sumatra was chosen to further investigate antimicrobials production. Isolates of hot spring bacteria that produced the highest antimicrobial were identified by comparing the similarity of the 16S rRNA gene sequences. BLAST result and phylogenetic tree showed that the selected thermophilic bacterial strain was similar to Bacillus paramicoides with the similarity index of 99.93%. Analysis of bioactive compounds of the ethyl acetate extract of liquid cultures of B. paramycoides LBKURCC218 showed the best producer of antimicrobial compounds compared to other isolates. The most identified compounds from the ethyl acetate extract were Dodecanoic acid, representing 23.62% of the total compounds, followed by 11-Dodecanoic acid at 17.84%. Ethyl acetate extract of B. paramycoides LBKURCC218 has a high inhibition zone against Escherichia coli, Staphylococcus aureus, and Candida albicans.

show abstract

“…Clot formation is a crucial procedure that avoids excessive blood vessel bleeding. Streptokinase (SK) is a thrombolytic medication and enzyme produced by a type of bacteria known as beta-hemolytic streptococci ( Abed et al, 2019). It is not a plasminogen activator by itself, but it binds to plasmin or free circulating plasminogen to form a complex that can change other plasminogen into plasmin.…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of native streptokinase in E. coli BL21(DE3)

Hameed

Al-Hayawi

Buniya

2022

ijhs

View full text Add to dashboard Cite

Streptokinase (SK) is a potent plasminogen activator naturally produced by beta-hemolytic streptococcus bacteria. Streptokinase is one of the most important thrombolytic agents that was frequently employed treatment of myocardial infarction. In this study, we amplified the SK gene isolated from Streptococcus pyogenes. The sequence of SK was recorded in the NCBI database. The Accession number of the SK sequence was LC625519.1. The SK gene was digested by EcoR1 and Hindlll, ligated with the linear pGEM®-3Zf (+) vector, and then introduced into an expression host E. coli BL21(DE3). For the production of the SK protein, we induced by 1mM IPTG. SDS-PAGE was used for the evaluation of the streptokinase synthesis with a molecular weight of around 47 kDa. Using the casein lysis method and the in vitro clot lysis assay, the activity of native SK was assessed. The native SK has clot lysis activity and a clear zone in the casein plate.

show abstract

Streptokinase Loading Fabrication Magnetic Nanoparticle Supported With Tannic Acid as a Modified Thrombolytic Agent

Cited by 4 publications

References 11 publications

Untitled

Untitled

Effect of Vitamin D3 Supplement on the Semen Quality in Human Patients with Vitamin D Deficiency

Cloning and expression of native streptokinase in E. coli BL21(DE3)

Contact Info

Product

Resources

About