Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction

Maragheh, Behnaz Faramarzian Azimi; Fatourachi, Parisa; Mohammadi, Seyede Momeneh; Valipour, Behnaz; Behtari, Meysam; Dehnad, Alireza; Charoudeh, Hojjatollah Nozad

doi:10.15171/apb.2018.076

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…Their presence is very important in the prevention of colonisation of more virulent micro-organisms [155] Streptococcus gordonii May have protective role, such that epithelial cells do not respond to Porphyromonas gingivalis-induced epithelial cell proliferation stimulation [156] Streptomyces Some species have been shown to inhibit cell growth [157], including apoptosis of cancer cell lines [158] Neisseria Can break down toxins of tobacco…”

Section: Streptococcus Mitismentioning

confidence: 99%

The Role of the Microbiome in Oral Squamous Cell Carcinoma with Insight into the Microbiome–Treatment Axis

Sami

Elimairi

Stanton

et al. 2020

IJMS

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Oral squamous cell carcinoma (OSCC) is one of the leading presentations of head and neck cancer (HNC). The first part of this review will describe the highlights of the oral microbiome in health and normal development while demonstrating how both the oral and gut microbiome can map OSCC development, progression, treatment and the potential side effects associated with its management. We then scope the dynamics of the various microorganisms of the oral cavity, including bacteria, mycoplasma, fungi, archaea and viruses, and describe the characteristic roles they may play in OSCC development. We also highlight how the human immunodeficiency viruses (HIV) may impinge on the host microbiome and increase the burden of oral premalignant lesions and OSCC in patients with HIV. Finally, we summarise current insights into the microbiome–treatment axis pertaining to OSCC, and show how the microbiome is affected by radiotherapy, chemotherapy, immunotherapy and also how these therapies are affected by the state of the microbiome, potentially determining the success or failure of some of these treatments.

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Section: Streptococcus Mitismentioning

confidence: 99%

The Role of the Microbiome in Oral Squamous Cell Carcinoma with Insight into the Microbiome–Treatment Axis

Sami

Elimairi

Stanton

et al. 2020

IJMS

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“…Cells were washed and stained with PI (5 μL/1 × 10 6 ) and immediately analyzed by flow cytometry. 20 In more detail, intracellular staining was performed for caspase-3. Cells were washed first with PBS, fixed with 4% formaldehyde for 30 min, resuspended in 0.5 mL of the Permeabilization solution, incubated for 20 min, washed with PBS, and incubated with anti-caspase-3 antibodies for 20 min.…”

Section: Apoptosis Assaymentioning

confidence: 99%

Inhibition of mitochondria induces apoptosis and reduces telomere length and activity in acute myeloid leukemia stem cells

Valipour,

Davari,

Farahzadi

et al. 2023

Cell Biochemistry & Function

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Acute myeloid leukemia (AML) is a highly lethal hematological malignancy in adults and children. Abnormal proliferation of leukemia stem cells (LSC) with CD34+ and CD38− phenotypes are the main clinical features of AML. Patients with AML face drug resistance and treatment failure due to a default in stem and progenitor cells. Therefore, defining LSC properties is necessary for targeting leukemia‐initiating cells. Mitochondrial mass and activity increase in AML initiating cells compared with normal stem cells. This idea has offered the inhibition of the mitochondrial translation machinery to reduce the number of leukemia‐initiating cells in patients with AML Tigecycline is an FDA‐approved microbial antibiotic that inhibits oxidative phosphorylation in mitochondria, resulting in the suppression of leukemia cell proliferation with little toxicity to normal cells. Thus, the present study was conducted to evaluate whether LSC is influenced by mitochondrial inhibition. We measured the IC50 of tigecycline in KG‐1a AML cell lines. KG‐1a AML cell lines were separated into CD34+ and CD34− cells by MACS. In the following, these cells were treated with 20 µM (IC50) tigecycline. The expression of Annexin/PI, Caspase 3, apoptotic genes (BCL2, BCLX, BAX, BAD, and P53) and proteins (P53, BAX, BCL2 and Caspase 9) was evaluated in CD34+, CD34− and KG‐1a AML cells. In addition, the telomere length and expression of hTERT were evaluated in this study. The results indicated that BCl2 (gene and protein) and BCLX gene dramatically decreased. In addition, BAD, BAX, and P53 gene and protein expression significantly increased in CD34+ AML cells compared to CD34− AML cells. The results also suggested that tigecycline induced intrinsic (Cleaved‐caspase 9/Pro‐Caspase 9 ratio) and p53‐mediated apoptosis. Furthermore, hTERT gene expression and telomere length decreased in the tigecycline‐treated groups. Taken together, our findings indicate that inhibition of mitochondrial activity with tigecycline can induce apoptosis in cancer stem cells and can be used as a novel method for cancer therapy.

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“…Ether extract of Streptomyces levis ABRIINW111 showed an increase in p53 expression, cell cycle arrest in G1 and G2/M phases and apoptosis in SW480 colon cancer cell line (Faramarzian et al 2018 ). Metabolites from this extract decreased anti-apoptotic K-RAS and Bcl-2 proteins expression and increased pro-apoptotic p53 protein expression (Fatourachi et al 2018 ).…”

Section: Anti-cancer Activity Of Bacterial Extractsmentioning

confidence: 99%

“…Metabolites derived from Streptomyces sp. SY-103 have been shown to induce apoptosis in human leukemic cells upon treatment; through activating caspase-3 and inactivating AKT (Faramarzian et al 2018 ). Some of these metabolites are pure cytotoxic compound (PCC), spicamycin and its derivative KRN5500, which were found to induce apoptosis by decreasing expression of Bcl-2 and modulating the normal localization pattern of promyelocytic leukemia protein (PML) from speckled pattern to microspeckled pattern in myeloid and lymphoid leukemic cells (Jeong et al 2010 ; Zhang et al 2000 ).…”

Section: Anti-cancer Activity Of Bacterial Extractsmentioning

confidence: 99%

Therapeutic applications and biological activities of bacterial bioactive extracts

et al. 2021

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Bacteria are rich in a wide variety of secondary metabolites, such as pigments, alkaloids, antibiotics, and others. These bioactive microbial products serve a great application in human and animal health. Their molecular diversity allows these natural products to possess several therapeutic attributes and biological functions. That’s why the current natural drug industry focuses on uncovering all the possible ailments and diseases that could be combated by bacterial extracts and their secondary metabolites. In this paper, we review the major utilizations of bacterial natural products for the treatment of cancer, inflammatory diseases, allergies, autoimmune diseases, infections and other diseases that threaten public health. We also elaborate on the identified biological activities of bacterial secondary metabolites including antibacterial, antifungal, antiviral and antioxidant activities all of which are essential nowadays with the emergence of drug-resistant microbial pathogens. Throughout this review, we discuss the possible mechanisms of actions in which bacterial-derived biologically active molecular entities could possess healing properties to inspire the development of new therapeutic agents in academia and industry.

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Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction

Cited by 9 publications

References 49 publications

The Role of the Microbiome in Oral Squamous Cell Carcinoma with Insight into the Microbiome–Treatment Axis

The Role of the Microbiome in Oral Squamous Cell Carcinoma with Insight into the Microbiome–Treatment Axis

Inhibition of mitochondria induces apoptosis and reduces telomere length and activity in acute myeloid leukemia stem cells

Therapeutic applications and biological activities of bacterial bioactive extracts

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