Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential

Abstract

A new Streptomyces species discovered from Sarawak mangrove soil is described, with the proposed name – Streptomyces monashensis sp. nov. (strain MUSC 1J T ). Taxonomy status of MUSC 1J T was determined via polyphasic approach. Phylogenetic and chemotaxonomic properties of strain MUSC 1J T were in accordance with those known for genus Streptomyces . Based on phylogenetic analyses, the strains closely related to MUSC 1J T were Streptomyces corchorusii DSM 40340 T (98.7%), Streptomyces olivaceoviridis NBRC 13066 T (98.7%), Streptomyces canarius NBRC 13431 T (98.6%) and Streptomyces coacervatus AS-0823 T (98.4%). Outcomes of DNA–DNA relatedness between strain MUSC 1J T and its closely related type strains covered from 19.7 ± 2.8% to 49.1 ± 4.3%. Strain MUSC 1J T has genome size of 10,254,857 bp with DNA G + C content of 71 mol%. MUSC 1J T extract exhibited strong antioxidative activity up to 83.80 ± 4.80% in the SOD assay, with significant cytotoxic effect against colon cancer cell lines HCT-116 and SW480. Streptomyces monashensis MUSC 1J T (=DSM 103626 T = MCCC 1K03219 T ) could potentially be a producer of novel bioactive metabolites; hence discovery of this new species may be highly significant to the biopharmaceutical industry as it could lead to development of new and useful chemo-preventive drugs.

Investigating the antioxidant potential of Streptomyces sp. MUSC 11 from mangrove soil in Malaysia

“…It has been suggested that understudied ecosystem hold Streptomyces species which can meet the growing demand of drug discovery and development industry [77] . Researchers who made an effort to study Streptomyces from less explored ecosystems such as mangrove forest were able to discover novel Streptomyces species and Streptomyces strains showing potent antioxidant activities [40,42,66,68,78,79] . dards for potential match.…”

“…dards for potential match. Nowadays, GC-MS coupled with NIST mass spectral library is extensively used for determining the bioactive constituents in microbial extracts [42,68,79] . In this experiment, 11 compounds were detected in the methanolic extract MUSC 11: Interestingly 11 compounds were detected by GC-MS in the microbial crude extract MUSC 11 with wide ranging biological activities.…”

“…Further information on the nature of phenolic compound (3) was provided by the GC -MS analysis (Table 5 and Figure 3). Studies of Streptomyces involving antioxidant on numerous occasions have mentioned the detection phenolic compounds including compound (3) [41,42,48,66,68,79,111] . Hence, compound (3) was perceived to have caused the antioxidant activity to some extent, provided the contribution may have also come from other constituent in methanolic extract MUSC 11.…”

In silico genomic mining reveals unexplored bioactive potential of rare actinobacteria isolated from Egyptian soil

“…In silico prediction and characterization of a microbial secondary metabolite of biosynthetic gene clusters has successfully contributed to the development of new medicines (Loureiro et al 2018). The majority of these metabolites belong to a wide variety of chemical classes and have shown antibiotic and antitumor activities (Law et al 2019). Recently, the cost of genome sequencing has dramatically decreased and allows for the discovery of thousands of gene clusters encoding the biosynthetic machinery for these compounds (Palazzotto and Weber 2018).…”

Whole genome sequence of Streptomyces humi strain MUSC 119T isolated from intertidal soil

“…Owing to their ability in synthesizing bioactive compounds of various structures, members of Streptomyces have always been in the limelight for the search of pharmaceutically important compounds [1][2][3][4][5][6] . Coupled with their ability to synthesize various bioactive compounds, their unique life cycle and spore formation has allowed these filamentous bacteria to survive and colonize various habitats, covering both terrestrial and marine region [7][8][9][10][11][12][13][14][15][16][17][18] . Streptomyces humi MUSC 119 T was recovered from mangrove soil in east coast of Peninsular Malaysia [19] .…”