Streptomyces odonnellii sp. nov., a proteolytic streptomycete isolated from soil under cerrado (savanna) vegetation cover

Abstract

A novel streptomycete, strain 594, isolated from Brazilian soil collected under cerrado (savanna) vegetation cover is described. Strain 594 produced thermophilic chitinolytic proteases in assays containing feather meal and corn steep liquor as sole sources of carbon and nitrogen. The strain produced white to grey aerial mycelium and spiral chains of spiny-surfaced spores on the aerial mycelium and did not produce diffusible pigments. The ll-isomer of diaminopimelic acid was present in the cell wall and menaquinones were predominantly MK-9(H6) (52 %) and MK-9(H8) (30 %) with 6 % MK-9(H4) and slightly less than 1 % MK-9(H2). Polar lipids present were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The major fatty acids were anteiso-C15 : 0, anteiso-C16 : 0, anteiso-C14 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 70.4 mol%. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence indicated that it differed from described Streptomyces species. Multilocus sequence analysis (MLSA) using five housekeeping genes (atpD, gyrB, rpoB, recA and trpB) comparing Streptomyces type strains showed that the MLSA distance of strain 594 to the most closely related species was greater than the 0.007 threshold. The in silico DNA-DNA relatedness between the genome sequence of strain 594 and that of the phylogenetically nearest species was well below the species level recommendation. There was thus multiple evidence justifying the description of this strain as representing a novel species, for which the name Streptomyces odonnellii sp. nov. is proposed. The type strain is 594 (=IMPPG 594=DSM 41949=NRRL B-24891).

Investigating the antioxidant potential of Streptomyces sp. MUSC 11 from mangrove soil in Malaysia

“…The filamentous, aerobic, soil-dwelling, gram-positive Streptomyces bacteria [24] have been found residing in soil samples collected from many countries [72][73][74][75][76] . The traditional practice regarding the isolation of Streptomyces from soil samples, have over the years resulted in the rediscovery of compounds which slowly exhausted the supplies of new compounds.…”

Antiplasmodial activity, biosynthetic gene clusters diversity, and secondary metabolite constituent of selected Indonesian Streptomyces

“…SHP 22-7 was closely related to S. rochei strain NRRL B-1559 T which known to have the ability to produce macrocylic antibiotic (Arakawa et al 2005). In contrast, GMY01 as marine bacterium was closely related to S. odonnellii strain 594 T which was isolated from savanna soil (Pereira et al 2017). The four reference Streptomyces were not able to produce antiplasmodial compounds.…”