Streptomyces puniceus strain AS13., Production, characterization and evaluation of bioactive metabolites: A new face of dinactin as an antitumor antibiotic

Hussain, Aashiq; Rather, Muzafar Ahmad; Dar, Mohd Saleem; Dangroo, Nisar A.; Aga, Mushtaq A.; Qayum, Arem; Shah, Aabid Manzoor; Ahmad, Zahoor; Hassan, Qazi Parvaiz

doi:10.1016/j.micres.2017.12.004

Cited by 35 publications

(25 citation statements)

References 29 publications

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“…(Wink); dinactin, 1-(2,4-dihydroxy-6methylphenyl)-ethanone(Hussain et al, 2018) 54VY13 (S. albidoflavus)Yan et al, 2010;Charousová et al, 2016c) 77VY13 (S. werraensis) hydroxybutyrate) depolymerase(García-Hidalgo et al, 2012)…”

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confidence: 99%

Soil Amended With Organic Fertilizers as a Source of Actinomycetes With High Potential as Biocontrol Agents

Javoreková¹,

Kovácsová²,

Medo³

et al. 2019

J microb biotech food sci

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MATERIAL AND METHODS Sample collection Actinomycetes were isolated from soil (Haplic Fluvisol) located near Nitra (Slovakia-latitude 48° 31' 35.97'' N, longitude 18° 11' 46.82'' E), one compost, one vermicompost and soil amended with compost or vermicompost. Vermicompost and compost were applied to the soil at a total dose of 20 Mg.ha −1. The applied compost was prepared by mixing straw (11% by dry weightdw), hay (3% by dw), silage (32% by dw), sheep manure (9% by dw), cow manure (34% by dw) and soil from the subsoil (11% by dw). Vermicompost was prepared by mixing the compost (41% by dw), cow manure (20% by dw), peat and forest litter (21% by dw), soil (10% by dw), a compound of grass, straw and hay (6% by dw) and the fill vermin (2% by dw). The fill vermin (older vermicompost) containing earthworms and cocoons of earthworms (70 earthworms dm −3) was added to the compost. The basic chemical and microbial characteristics of soil samples and both composts (Table 1) were determined according to the defined values: oxidizable carbon (Cox) by the Tiurin method, total nitrogen (Nt) by the Kjeldahl distillation method, pH (H2O) and microbial biomass carbon (Cmic), which was determined by the fumigation-extraction method of Vance et al. (1987). Plant diseases caused by pathogenic fungi are a severe problem for agriculture, and some organic fertilizers have shown a suppressive capacity due to the antagonistic action of microbial communities inhabiting those substrates. The purpose of this work was to isolate and identify actinomycetes from soil, compost and vermicompost able to antagonize phytopathogens. In total, out of 352 actinomycetes successfully recovered on Pochon medium and of which were selected representative 22 mycelium forming isolates, which were subjected to morphological and genotypic characterization. Genetic characterization based on 16S rDNA fragment sequencing revealed that, all the actinomycete isolates belong to the genus Streptomyces. The antifungal activity of isolates was tested against eight phytopathogenic fungi, and they were most activated against Rhizoctonia solani, Alternaria tenuissima, Aspergillus niger and Penicillium expansum. Isolates 51VK13 (Streptomyces sampsonii) and 12VK13 (Streptomyces flavovariabilis) isolated from soil amended with vermicompost showed the greatest antagonistic activity. Their bioactive potential was also confirmed by presence of genes for nonribosomal peptide synthetase (NRPS) and polyketide synthase types I and II (PKS-I and PKS-II). It can, therefore, be concluded that soil amended with organic fertilizers such as compost, and in particular vermicompost, provides a lower incidence of phytopathogens, especially fungi. Isolated actinomycetes have also shown high potential for the production of bioactive compounds useful in the control of plant diseases. ARTICLE INFO

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confidence: 99%

Soil Amended With Organic Fertilizers as a Source of Actinomycetes With High Potential as Biocontrol Agents

Javoreková¹,

Kovácsová²,

Medo³

et al. 2019

J microb biotech food sci

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“…Microorganisms such as the soil bacterium Streptomyces puniceus and the vaginal protozoan Trichomonas vaginalis had a negative effect on the reproductive ability of epithelial cells [9,27]. While the microorganisms we studied proved to impede migration and proliferation of oral epithelial cells in a dose-dependent way [2,8,13], it is unlikely that this inhibition of wound healing was caused by damage to the ability of oral epithelial cells to undergo unlimited cell division after exposure to these microorganisms.…”

Section: Discussionmentioning

confidence: 89%

“…The ability to proliferate continuously is essential for tissue integrity and function [5]. Although the assay is originally designed to study the effect of irradiation, others successfully used this assay to study the effect of microorganisms on the reproductive capacity of cells [9,27].…”

Section: Discussionmentioning

confidence: 99%

Exploring the role of oral microorganisms in the pathogenesis of mucositis by assessing their impact on metabolic activity and reproductive capacity of epithelial cells in vitro

Haverman

Laheij

Nie

et al. 2020

Support Care Cancer

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Purpose Clinical and in vitro studies showed selected oral microorganisms to be related to delayed wound healing and ulcerative oral mucositis. However, it is not known whether this effect is due to reduced metabolism and/or the reduced reproductive capacity of epithelial cells. Therefore, we studied the influence of the oral microorganisms Porphyromonas gingivalis, Candida glabrata, and Candida kefyr on cell metabolism and reproductive capacity of oral epithelial cells, aimed to further unravel the pathogenesis of oral mucositis. Methods Oral epithelial cells were exposed to different concentrations of P. gingivalis, C. glabrata, and C. kefyr as monoinfections or mixed together. An MTT assay was performed to determine the effect on cell metabolism. A clonogenic assay was used to study the effect on the reproductive capacity of oral epithelial cells. Results The metabolism of oral epithelial cells was reduced when the microorganisms were present in high concentrations: P. gingivalis at a multiplicity of infection (MOI) of 1000 and the Candida spp. at MOI 100. No statistical difference was observed in the ability of a single epithelial cell to grow into a colony of cells between control and P. gingivalis, C. glabrata, and C. kefyr, independent of the concentrations and combinations used. Conclusion P. gingivalis, C. glabrata, and C. kefyr lowered the metabolic activity of oral epithelial cells in high concentrations, yet they did not influence the reproductive capacity of epithelial cells. Their impact on ulcerative oral mucositis is likely due to an effect on the migration, proliferation, and metabolism of epithelial cells.

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“…A potent vasodilating activity has been reported for mer-A2026B ( 1 ) [34]; and insecticidal, antimicrobial and cytotoxic activities for piericidin-A 1 ( 2 ) [35–37]; while strong antibacterial and anticancer activities have been found for nigericin ( 3 ) and dinactin ( 4 ) [38–40]. The high activities and good selectivity indexes obtained for nigericin ( 3 ) and dinactin ( 4 ) in our experiments ( Table 1 ) are in agreement with previous data for nigericin monosodium salt and nonactin, an analogue of 4 , using ex-vivo splenic explant culture system from hamsters infected with L .…”

Section: Resultsmentioning

confidence: 99%

Antifungal compounds from Streptomyces associated with attine ants also inhibit Leishmania donovani

et al. 2019

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Bacterial strains isolated from attine ants showed activity against the insect specialized fungal pathogen Escovopsis and also against the human protozoan parasite Leishmania donovani . The bioassay guided fractionation of extracts from cultures of Streptomyces sp. ICBG292, isolated from the exoskeleton of Cyphomyrmex workers, led to the isolation of Mer-A2026B ( 1 ), piericidin-A 1 ( 2 ) and nigericin ( 3 ). Nigericin ( 3 ) presented high activity against intracellular amastigotes of L . donovani (IC 50 0.129 ± 0.008 μM). Streptomyces puniceus ICBG378, isolated from workers of Acromyrmex rugosus rugosus , produced dinactin ( 4 ) with potent anti- L . donovani activity against intracellular amastigotes (IC 50 0.018 ± 0.003 μM). Compounds 3 and 4 showed good selectivity indexes, 88.91 and 656.11 respectively, and were more active than positive control, miltefosine. Compounds 1 – 4 were also active against some Escovopsis strains. Compounds 1 and 2 were also produced by Streptomyces sp. ICBG233, isolated from workers of Atta sexdens , and detected in ants’ extracts by mass spectrometry, suggesting they are produced in the natural environment as defensive compounds involved in the symbiotic interaction.

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Streptomyces puniceus strain AS13., Production, characterization and evaluation of bioactive metabolites: A new face of dinactin as an antitumor antibiotic

Cited by 35 publications

References 29 publications

Soil Amended With Organic Fertilizers as a Source of Actinomycetes With High Potential as Biocontrol Agents

Soil Amended With Organic Fertilizers as a Source of Actinomycetes With High Potential as Biocontrol Agents

Exploring the role of oral microorganisms in the pathogenesis of mucositis by assessing their impact on metabolic activity and reproductive capacity of epithelial cells in vitro

Antifungal compounds from Streptomyces associated with attine ants also inhibit Leishmania donovani

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