Streptomyces urticae sp. nov., isolated from rhizosphere soil of Urtica urens L.

Abstract

Two novel Gram-stain positive, spore-forming, aerobic actinomycetes, designated NEAU-PCY-1 and NEAU-PCY-2, were isolated from rhizosphere soil of Urtica urens L. collected from Anshan, Liaoning Province, northeast China. The 16S rRNA gene sequence analysis showed that strains NEAU-PCY-1 and NEAU-PCY-2 exhibited 99.8% similarity with each other and are closely related to Streptomyces abietis DSM 42080 (98.2, 98.3%) and Streptomyces fildesensis DSM 41987 (98.0, 98.1%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains formed a cluster with these two closely related species. Moreover, DNA-DNA hybridization results and some phenotypic, physiological and biochemical properties differentiated the two strains from their close relatives in the genus Streptomyces. Based on a polyphasic taxonomy study, strains NEAU-PCY-1 and NEAU-PCY-2 are considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces urticae sp. nov. is proposed, with NEAU-PCY-1 (= DSM 105115 = CCTCC AA 2017015) as the type strain.

Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective

“…Different pretreatment techniques have also been applied to samples, such as ultrasonic treatment [140], orbital shaking [166] and water bath sonication [163], and are crucial to activate Streptomycete spores and inhibit the growth of unwanted microbes [168] (Table 5). Subjecting the sample to heat treatment for 1 h at 120 °C [146] is an important pre-treatment because actinomycete spores, including Streptomycetes, are more resistant to desiccation than other facultative and Gram-negative anaerobic bacteria [94].…”

“…• C for 21 days [140] Ultrasonic treatment (160W) for 3 min followed by incubation of soil sample at 28 Head of an ant sodium succinateasparagine agar at pH 7.2 and supplemented with cycloheximide (50 mg L −1 ) and nalidixic acid (20 mg L −1 ).…”

Streptomyces cahuitamycinicus sp. nov., isolated from desert soil and reclassification of Streptomyces galilaeus as a later heterotypic synonym of Streptomyces bobili

“…This genus is responsible for a significant portion of all isolated and identified natural compounds such as antibiotics [10, 11]. For this reason, the genus Streptomyces has become the main focus of isolation and investigation studies of the novel species from different habitats such as deserts [12–15], bats [16], fruits [17], insects [18, 19], lake sediments [20, 21], manganese-contaminated soil [22], mangrove sediments [23, 24], mangrove soil [25], marine sponges [26, 27], marine sediments [28, 29], rhizospheres [30, 31], zinc and copper mine [32], and plant tissues [33, 34] and soil [35, 36]. In this study, it was aimed to isolate novel actinobacteria from soil samples collected from the Karakum Desert.…”

Streptomyces durocortorensis sp. nov., isolated from oak rhizosphere

“…As major plant endophytes, they are notably active in the rhizosphere [9]. Numerous Streptomyces species were discovered in rhizospheres of wild plants (nettle) [10], of agricultural crops (maize, wheat) [11,12] or of trees (populous, paper mulberry) [13,14]. In such environments, they play a major role in phosphate solubilization, nitrogen fixation and other nutrition processes [15].…”