Streptovirundins, new antibiotics with antibacterial and antiviral activity. I. Culture taxonomy, fermentation and production of streptovirudin complex.

Thrum, H.; Eckardt, K.; Bradler, Gertraud; Fügner, R; Tonew, E; Tonew, M

doi:10.7164/antibiotics.28.514

Cited by 27 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…92 The length and composition of this chain distinguishes one member of the tunicamycin family from another. Whilst the initial characterisation and development of tunicamycin was ongoing, the streptoviridins 94 and the corynetoxins 95 were isolated and characterised (Fig. 11).…”

Section: Tunicamycins Streptoviridins and Corynetoxinsmentioning

confidence: 99%

Antimicrobial nucleoside antibiotics targeting cell wall assembly: Recent advances in structure–function studies and nucleoside biosynthesis

et al. 2010

View full text Add to dashboard Cite

The quest for new antibiotics, especially those with activity against Gram-negative bacteria, is urgent; however, very few new antibiotics have been marketed in the last 40 years, with this limited number falling into only four new structural classes. Several nucleoside natural product antibiotics target bacterial translocase MraY, involved in the lipid-linked cycle of peptidoglycan biosynthesis, and fungal chitin synthase. Biosynthetic studies on the nikkomycin, caprazamycin and pacidamycin/mureidomycin families are also reviewed.

show abstract

Section: Tunicamycins Streptoviridins and Corynetoxinsmentioning

confidence: 99%

Antimicrobial nucleoside antibiotics targeting cell wall assembly: Recent advances in structure–function studies and nucleoside biosynthesis

et al. 2010

View full text Add to dashboard Cite

show abstract

“…1 Their structures consist of an unusual eleven carbon aminodialdose core (tunicamine) to which uracil and N-acetylglucosamine (GlcNAc) are anomerically attached, alongside a range of amide-linked unsaturated fatty acids (Figure 1). 2 A number of related natural products belong to the tunicamycin family, namely streptovirudins, 3 corynetoxins, 4 MM19290, 5 mycospocidin 6 and antibiotic 24010. 7 All share the conserved carbohydrate core and presumably have similar biosynthetic pathways, but the genes required for their production have not been identified.…”

Section: Introductionmentioning

confidence: 99%

Dissecting tunicamycin biosynthesis by genome mining: cloning and heterologous expression of a minimal gene cluster

et al. 2010

View full text Add to dashboard Cite

Tunicamycin nucleoside antibiotics were the first known to target the formation of peptidoglycan precursor lipid I in bacterial cell wall biosynthesis. They have also been used extensively as inhibitors of protein N-glycosylation in eukaryotes, blocking the biogenesis of early intermediate dolichylpyrophosphoryl-N-acetylglucosamine. Despite their unusual structures and useful activities, little is known about their biosynthesis. Here we report identification of the tunicamycin biosynthetic genes in Streptomyces chartreusis following genome sequencing and a chemically-guided strategy for in silico genome mining that allowed rapid identification and unification of an operon fractured across contigs. Heterologous expression established a likely minimal gene set necessary for antibiotic production, from which a detailed metabolic pathway for tunicamycin biosynthesis is proposed. These studies unlock a comprehensive and unusual toolbox of biosynthetic machinery with which to create variants of this important natural product, allowing possible improved understanding of the mode of action and facilitating future redesign. We anticipate that these results will enable the generation of altered specific inhibitors of diverse carbohydrate-processing enzymes, including improved targeting of lipid I biosynthesis.

show abstract

“…Streptovirudins are nucleoside antibiotics and antivirals produced by Streptomyces griseoflavus subsp. thuringiensis [ 51 ], and some of them were also identified during the current analysis. Streptovirudin C1 ( 17 , m/z 819.42) and streptovirudin A1 ( 18 , m/z 791.393) are classified as series I streptovirudins [ 52 ] and were only detected in extracts from the S. clavuligerus strain overexpressing frr ( Figure 4 A and Figure S4 ; Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Specialized Metabolites from Ribosome Engineered Strains of Streptomyces clavuligerus

et al. 2021

View full text Add to dashboard Cite

Bacterial specialized metabolites are of immense importance because of their medicinal, industrial, and agricultural applications. Streptomyces clavuligerus is a known producer of such compounds; however, much of its metabolic potential remains unknown, as many associated biosynthetic gene clusters are silent or expressed at low levels. The overexpression of ribosome recycling factor (frr) and ribosome engineering (induced rpsL mutations) in other Streptomyces spp. has been reported to increase the production of known specialized metabolites. Therefore, we used an overexpression strategy in combination with untargeted metabolomics, molecular networking, and in silico analysis to annotate 28 metabolites in the current study, which have not been reported previously in S. clavuligerus. Many of the newly described metabolites are commonly found in plants, further alluding to the ability of S. clavuligerus to produce such compounds under specific conditions. In addition, the manipulation of frr and rpsL led to different metabolite production profiles in most cases. Known and putative gene clusters associated with the production of the observed compounds are also discussed. This work suggests that the combination of traditional strain engineering and recently developed metabolomics technologies together can provide rapid and cost-effective strategies to further speed up the discovery of novel natural products.

show abstract

Streptovirundins, new antibiotics with antibacterial and antiviral activity. I. Culture taxonomy, fermentation and production of streptovirudin complex.

Cited by 27 publications

References 0 publications

Antimicrobial nucleoside antibiotics targeting cell wall assembly: Recent advances in structure–function studies and nucleoside biosynthesis

Antimicrobial nucleoside antibiotics targeting cell wall assembly: Recent advances in structure–function studies and nucleoside biosynthesis

Dissecting tunicamycin biosynthesis by genome mining: cloning and heterologous expression of a minimal gene cluster

Specialized Metabolites from Ribosome Engineered Strains of Streptomyces clavuligerus

Contact Info

Product

Resources

About