Stress-induced increment on total bronchoalveolar cell count in OVA-sensitized rats

Portela, Carlos de Paula; Massoco, Cristina de Oliveira; Lima, Wothan Tavares de; Palermo-Neto, João

doi:10.1016/s0031-9384(00)00429-7

Cited by 32 publications

(2 citation statements)

References 42 publications

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“…To investigate possible physiological mechanisms underlying an injurious effect of stress on asthma, animal models of asthma have been commonly used (10)(11)(12)(13)(14)(15)(16). However, such asthma models were mainly restricted to an experimental design in which adult animals were exposed to stress during antigen sensitization or during antigen challenge after sensitization, with only one pilot study showing that prenatal psychological stress exacerbated adult asthma (16).…”

mentioning

confidence: 99%

Early-Life Psychological Stress Exacerbates Adult Mouse Asthma via the Hypothalamus–Pituitary–Adrenal Axis

Chida

Sudo²,

Sonoda

et al. 2007

Am J Respir Crit Care Med

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confidence: 99%

Early-Life Psychological Stress Exacerbates Adult Mouse Asthma via the Hypothalamus–Pituitary–Adrenal Axis

Chida

Sudo²,

Sonoda

et al. 2007

Am J Respir Crit Care Med

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“…However, they also found lower neutrophil counts in the male rats 16. Another study concluded that this difference in cell counts between males and females could be attributed to stress affecting one gender disproportionately 17. In other words, this variability in the data could be attributed to the length of the treatment and the age of the animals.…”

Section: Discussionmentioning

confidence: 94%

The effects of individually ventilated cages on the respiratory systems of male and female Wistar rats from birth until adulthood

Marchesi¹,

Soto²,

Castro³

et al. 2017

Clinics

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OBJECTIVE:To evaluate the respiratory systems of male and female rats maintained in individually ventilated cages (IVCs) from birth until adulthood.METHODS:Female Wistar rats were housed in individually ventilated cages or conventional cages (CCs) and mated with male Wistar rats. After birth and weaning, the male offspring were separated from the females and kept in cages of the same type until 12 weeks of age.RESULTS:The level of food consumption was lower in male offspring (IVC=171.7±9; CC=193.1±20) than in female offspring (IVC=100.6±7; CC=123.4±0.4), whereas the water intake was higher in female offspring (IVC=149.8±11; CC=99.2±0) than in male offspring (IVC=302.5±25; CC=249.7±22) at 11 weeks of age when housed in IVCs. The cage temperature was higher in individually ventilated cages than in conventional cages for both male (IVCs=25.9±0.5; CCs=22.95±0.3) and female (IVCs=26.2±0.3; CCs=23.1±0.3) offspring. The respiratory resistance (IVC=68.8±2.8; CC=50.6±3.0) and elastance (IVC=42.0±3.9; CC=32.4±2.0) at 300 µm/kg were higher in the female offspring housed in ventilated cages. The ciliary beat values were lower in both the male (IVCs=13.4±0.2; CC=15±0.4) and female (IVC=13.5±0.4; CC=15.9±0.6) offspring housed in individually ventilated cages than in those housed in conventional cages. The total cell (IVC=117.5±9.7; CC=285.0±22.8), neutrophil (IVC=13.1±4.8; CC=75.6±4.1) and macrophage (IVC=95.2±11.8; CC=170.0±18.8) counts in the bronchoalveolar lavage fluid were lower in the female offspring housed in individually ventilated cages than in those housed in conventional cages.CONCLUSIONS:The environmental conditions that exist in individually ventilated cages should be considered when interpreting the results of studies involving laboratory animals. In this study, we observed gender dimorphism in both the water consumption and respiratory mechanics of rats kept in ventilated cages.

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Anti‐asthmatic and anxiolytic effects ofHerissantia tiubae, a Brazilian medicinal plant

Monteiro

Costa

Vieira

et al. 2016

Immunity Inflam & Disease

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Herissantia tiubae (HtE) is a Brazilian plant used in folk medicine to treat inflammatory diseases. Our aim was to determine whether the HtE has anti‐inflammatory and anxiolytic effects in a murine model of asthma. Ovalbumin (OVA)‐sensitized BALB/c mice were treated with HtE (50, 100, or 200 mg/kg) or dexamethasone before each OVA challenge. After the last challenge, animals were subjected to anxiety tests and respiratory measurements. Following euthanasia, we quantified immune cells in the bronchoalveolar lavage (BAL), serum IgE titer and cytokine levels, cellular infiltration and mucus content in the lung tissues, and cellular composition of the mediastinal lymph nodes. OVA challenge in sensitized animals caused: (1) reduction of mean respiratory and dominant respiratory rate (from 398 ± 12 to 286 ± 20 cicles per minute (cpm) and from 320 ± 14 to 162 ± 15 cpm, respectively); (2) increase in behavioral markers of anxiety tests; (3) substantial pro‐inflammatory effects, including rise in OVA‐specific IgE titer (from 0 to 1:2048) and these inflammatory effect diminished the titer to 1:512 after HtE treatment; rise in plasma IL‐13 (from 13 ng/mL in saline to 227 ng/mL in OVA and HtE treatment restored to 1.29 ng/mL; rise in total BAL cell count (from 0.742 cells/mL in saline to 11.77 cells/mL in OVA), with prominent eosinophilia. H. tiubae extract affected respiratory parameters similarly to aminophylline, behavioral changes comparable to diazepam, and inflammation being as efficient as dexamethasone. H. tiubae extract (HtE) possesses both anti‐inflammatory and anxiolytic properties in the murine model of asthma.

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Stress-induced increment on total bronchoalveolar cell count in OVA-sensitized rats

Cited by 32 publications

References 42 publications

Early-Life Psychological Stress Exacerbates Adult Mouse Asthma via the Hypothalamus–Pituitary–Adrenal Axis

Early-Life Psychological Stress Exacerbates Adult Mouse Asthma via the Hypothalamus–Pituitary–Adrenal Axis

The effects of individually ventilated cages on the respiratory systems of male and female Wistar rats from birth until adulthood

Anti‐asthmatic and anxiolytic effects ofHerissantia tiubae, a Brazilian medicinal plant

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