The nonlinear, anisotropic, and multiscale mechanical behavior of knitted textiles is investigated experimentally in this article. The approach is motivated by recent computational work by the authors that revealed, for the first time to their best knowledge, local‐global mechanical behavior effects related to the hierarchical, three‐dimensional structure of this type of materials. The investigation is carried out on single jersey knitted textile specimens. Mechanical testing consisting of tensile loading along the two principal directions was coupled with a noncontact, optical metrology method capable of providing deformation measurements. The effect of globally applied loading on yarn‐to‐yarn interactions was explored using measured data. The results validate the previously obtained computational findings that include the anisotropic behavior between course and wale directions, the pronounced out‐of‐plane motion observed when in‐plane loading is applied, as well as the characteristic nonlinear mechanical behavior of knitted textiles. These effects were linked to direct observations of the loop structure that demonstrated the coupling between local kinematics and kinetics with global mechanical behavior.