Stress Tolerance of &lt;i&gt;Methylobacterium&lt;/i&gt; Biofilms in Bathrooms

Yano, Takehisa; Kubota, Hiromi; Hanai, Junya; Hitomi, Jun; Tokuda, Harukuni

doi:10.1264/jsme2.me12146

Cited by 53 publications

(43 citation statements)

References 49 publications

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“…Methylobacterium sp. is well adapted to survive in a filtration environment due to its resistance to cleaning agents, which makes it the principal bacterium identified in pink bathroom biofilms (Yano et al 2013).…”

Section: The Effect Of Operating Conditionsmentioning

confidence: 99%

A sequencing approach targeting the 16S rRNA gene unravels the biofilm composition of spiral-wound membranes used in the dairy industry

Chamberland

Lessard

Doyen

et al. 2016

Dairy Sci. & Technol.

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Few data are available concerning the composition of biofilms found at the surface of filtration membranes, which, to some extent, explains the long-term failure of numerous strategies developed to control biofouling. This preliminary study intended to design a metagenomic tool targeting the 16S rRNA gene in order to unravel a general portrait of bacterial communities found on spiral-wound membranes used in the dairy industry. A total of seven spiral-wound membrane elements (ultrafiltration, nanofiltration, or reverse osmosis) at the end of their useful lifetimes were collected from different dairy plants. Targeted analysis of the 16S rRNA genes of the metagenome extracted from the membranes revealed their bacterial diversity via high-throughput sequencing technology (Miseq, Illumina). It was found that the nature of the filtered fluid (milk, whey, water) explained 58.6 % of the variance observed between communities found on membranes. Treatments applied on dairy fluids (milk pasteurization, whey bleaching or whey ultrafiltration) induced a selective pressure that affected the diversity of bacterial communities found on membranes and the proportions of spore-former bacteria among them. This work provides the first complete bacterial portrait of the biofilm composition of spiral-wound membranes used in the dairy industry. It suggests that the nature of the filtered fluid and potentially filtration Dairy Sci. & Technol. (2017)

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Section: The Effect Of Operating Conditionsmentioning

confidence: 99%

A sequencing approach targeting the 16S rRNA gene unravels the biofilm composition of spiral-wound membranes used in the dairy industry

Chamberland

Lessard

Doyen

et al. 2016

Dairy Sci. & Technol.

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“…According to the literature, Methylobacterium has a strong biofilm-producing ability (28)(29)(30). Simões et al (29) tested the effects of sodium hypochlorite (liquid bleach) on the activity and culturability of Methylobacterium biofilms.…”

Section: Impact Of Biofilm Formation By Methylobacteriummentioning

confidence: 99%

Methylobacterium and Its Role in Health Care-Associated Infection

2014

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bMethylobacterium species are a cause of health care-associated infection, including infections in immunocompromised hosts. The ability of Methylobacterium species to form biofilms and to develop resistance to high temperatures, drying, and disinfecting agents may explain the colonization of Methylobacterium in the hospital environment in, e.g., endoscopes. Due to its slow growth, it can be easily missed during microbiological surveillance of endoscope reprocessing. The purpose of this minireview is to present an overview of documented infections and cross-contaminations with Methylobacterium related to endoscopic procedures and to illustrate the health care-associated relevance of this slow-growing bacterium.

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“…As has already been stated, Methylobacterium is resistant to treatments with chlorine Furuhata and Koike, 1993 , detergents Yano et al, 2012, desiccation Romanovskaya et al, 2002Yano et al, 2012 andUV Romanovskaya et al, 2002 and Rhodotorula showed tolerance to ROS Sasaki et al, 2000 . M. mesophilicum has spirilloxanthin and carotenoic acids Saito et al, 1995 and R. mucilaginosa has torularhodin, -catoten, and torulene Martin et al, 2010 .…”

Section: Bactericidal and Fungicidal Effects Of Hydrogen Peroxidementioning

confidence: 89%

“…Ihara et al, 2014;Scott et al, 2004;Yano et al, 2012 and the red-pigment producing yeasts, Rhodotorula spp. Ihara et al, 2014;Hamada and Fujita, 2001;Yano et al, 2012 . The efficacies of treatments against Methylobacterium spp.…”

Section: Introductionmentioning

confidence: 99%

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Killing Effect of Peppermint Vapor against Pink-Slime Forming Microorganisms

Ihara¹,

Sakamoto

Yoshida

et al. 2015

Biocontrol Sci.

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The killing effect of peppermint vapor PMV against pink-slime forming microorganisms, Methylobacterium mesophilicum as a bacterium and Rhodotorula mucilaginosa as a yeast, was investigated by the agar vapor assay. In this method, microbial cells were spread over the agar surface exposed to PMV in a petri dish, and then transferred into a recovery liquid. When 60 l of the peppermint liquid was added to a paper disc, a marked killing effect of PMV was observed after 48h against M. mesophilicum and after 168h against R. mucilaginosa. M. mesophilicum and R. mucilaginosa were found to be more resistant to PMV than Escherichia coli and Candida albicans, used as reference microorganisms, respectively. With the addition of 0.03% sodium pyruvate as a hydrogen peroxide scavenger in agar, the killing effect of PMV against E. coli and C. albicans was decreased, whereas it was little changed against M. mesophilicum and R. mucilaginosa. In fact, the properties of the killing effect of hydrogen peroxide solution at 0.2-1.0mM was in accord with those of PMV. M. mesophilicum and R. mucilaginosa were more resistant to the oxidant than E. coli and C. albicans, respectively. Results obtained suggested that reactive oxygen species ROS may be involved in the killing action of PMV and therefore pink-slime formers are more resistant to PMV than non-pink-slime formers because of the presence of carotenoids as an antioxidant in cells. We also suggest that the use of PMV appeared to be a potential tool for the control of pink-slime forming microorganisms occurring in wet areas of houses such as the bathroom and washing room.

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Stress Tolerance of <i>Methylobacterium</i> Biofilms in Bathrooms

Cited by 53 publications

References 49 publications

A sequencing approach targeting the 16S rRNA gene unravels the biofilm composition of spiral-wound membranes used in the dairy industry

A sequencing approach targeting the 16S rRNA gene unravels the biofilm composition of spiral-wound membranes used in the dairy industry

Methylobacterium and Its Role in Health Care-Associated Infection

Killing Effect of Peppermint Vapor against Pink-Slime Forming Microorganisms

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