Stress-triggered activation of gene expression in catecholaminergic systems: dynamics of transcriptional events

Sabban, Esther L.; Květňanský, Richard

doi:10.1016/s0166-2236(00)01687-8

Cited by 262 publications

(249 citation statements)

References 76 publications

Supporting

Mentioning

233

Contrasting

Unclassified

Order By: Relevance

“…For example, levels of tyrosine hydroxylase immunoreactivity are elevated in the LC of major depressive subjects and suicide victims compared to normal control subjects (Zhu et al, 1999). In animals, tyrosine hydroxylase mRNA is increased in the LC even after a single stressor, although increases in tyrosine hydroxylase protein require repeated stressors (Melia et al, 1992;Nestler et al, 1999;Sabban and Kvetnansky, 2001). Interestingly, repeated administration of CRF results in the upregulation of tyrosine hydroxylase in the LC (Melia and Duman, 1991).…”

Section: Discussionmentioning

confidence: 99%

Elevated Concentrations of CRF in the Locus Coeruleus of Depressed Subjects

Bissette

Klimek

Pan

et al. 2003

Neuropsychopharmacol

132

View full text Add to dashboard Cite

Research evidence that corticotropin-releasing factor (CRF) plays a role in the pathophysiology of major depressive disorder (MDD) has accumulated over the past 20 years. The elevation of lumbar cerebrospinal fluid (CSF) concentrations of CRF decreased responsiveness of pituitary CRF receptors to challenge with synthetic CRF, and increased levels of serum cortisol in MDD subjects support the hypothesis that CRF is chronically hypersecreted in at least the endocrine circuits of the hypothalamic-pituitary-adrenal (HPA) axis and may also involve other CRF brain circuits mediating emotional responses and/or arousal. One such circuit includes the excitatory CRF input to the locus coeruleus (LC), the major source of norepinephrine in the brain. Furthermore, there are now reports of decreased levels of CRF in lumbar CSF from MDD patients after symptom relief from chronic treatment with antidepressant drugs or electroconvulsive therapy. Whether this normalization reflects therapeutic effects on both endocrine-and limbic-associated CRF circuits has not yet been effectively addressed. In this brief report, we describe increased concentrations of CRF-like immunoreactivity in micropunches of post-mortem LC from subjects with MDD symptoms as established by retrospective psychiatric diagnosis compared to nondepressed subjects matched for age and sex.

show abstract

Section: Discussionmentioning

confidence: 99%

Elevated Concentrations of CRF in the Locus Coeruleus of Depressed Subjects

Bissette

Klimek

Pan

et al. 2003

Neuropsychopharmacol

132

View full text Add to dashboard Cite

show abstract

“…Repeated stress like immobilization periods of 2 h and hypoxia are shown to produce an immediate release of catecholamines (adrenaline, noradrenaline, and dopamine) in the adrenal medulla. This is followed by a fast upregulation of catecholamine-synthesizing enzymes (TH, DBH, and PNMT) and an increased expression of a number of genes encoding the transcription factors for TH, DBH, and PNMT mRNA synthesis plus other genes related to kinases/phosphatase signaling and neurosecretion [56].…”

Section: T-type Channels and Fast Capacitance Changes In Rat Melanotrmentioning

confidence: 99%

“…The cAMP-response element binding protein is involved in the rapid induction of TH and DBH transcription in chromaffin cells [56], and cAMP triggers the rise of the PNMT-promoter-driven gene activity in PC12 cells in hypoxic conditions [62]. Thus, exposure to cAMP, chronic hypoxia, and β 1 -adrenergic stimulations (which raise the level of cAMP) appear to be the suitable factors for mimicking prolonged stress conditions in vitro.…”

Section: T-type Channels and Fast Capacitance Changes In Rat Melanotrmentioning

confidence: 99%

T-type channels-secretion coupling: evidence for a fast low-threshold exocytosis

Carbone¹,

Marcantoni²,

Giancippoli³

et al. 2006

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

T-type channels are transient low-voltage-activated (LVA) Ca 2+ channels that control Ca 2+ entry in excitable cells during small depolarizations around resting potential. Studies in the past 20 years focused on the biophysical, physiological, and molecular characterization of T-type channels in most tissues. This led to a welldefined picture of the functional role of LVA channels in controlling low-threshold spikes, oscillatory cell activity, muscle contraction, hormone release, cell growth and differentiation. So far, little attention has been devoted to the role of T-type channels in transmitter release, which mainly involves channel types belonging to the highvoltage-activated (HVA) Ca 2+ channel family. However, evidence is accumulating in favor of a unique participation of T-type channels in fast transmitter release. Clear data are now reported in reciprocal synapses of the retina and olfactory bulb, synaptic contacts between primary afferent and second order nociceptive neurons, rhythmic inhibitory interneurons of invertebrates and clonal cell lines transfected with recombinant α 1 channel subunits. T-type channels also regulate the large dense-core vesicle release of neuroendocrine cells where Ca 2+ dependence, rate of vesicle release, and size of readily releasable pool appear comparable to those associated to HVA channels. This suggests that when sufficiently expressed and properly located near the release zones, T-type channels can trigger fast low-threshold secretion. In this study, we will review the main findings that assign a specific task to T-type channels in fast exocytosis, discussing their possible involvement in the control of the Ca 2+ -dependent processes regulating exocytosis like vesicle depletion and vesicle recycling.

show abstract

“…On the other hand, measurement of sympathoadrenal activity based on expression levels of mRNAs for catecholamine biosynthetic enzymes has been performed in the field of molecular biology (Nankova et al 1994;Nankova et al 1996;Brooks et al 1997;Levenson and Moore 1998;Rusnák et al 1998;Bornstein et al 1999;Lai et al 2000;Sabban and Kvetnanský 2001;Gallara et al 2004;Kubovcakova et al 2004;Klimes et al 2005;Lai et al 2005;Herradón et al 2006). However, earlier animal studies on some stresses such as immobilization, cold exposure, forced exercise, and insulininduced hypoglycemia (Brooks et al 1997;Lai et al 2000;Sabban and Kvetnanský 2001;Gallara et al 2004;Klimes et al 2005;Lai et al 2005), did not cover all forms of stresses encountered in forensic practice.…”

confidence: 99%

“…However, earlier animal studies on some stresses such as immobilization, cold exposure, forced exercise, and insulininduced hypoglycemia (Brooks et al 1997;Lai et al 2000;Sabban and Kvetnanský 2001;Gallara et al 2004;Klimes et al 2005;Lai et al 2005), did not cover all forms of stresses encountered in forensic practice. Moreover, most of the methods in the analysis including Southern blotting and semi-quantitative reverse trancrptional polymerase chain reaction (RT-PCR) (Nankova et al 1994;Nankova et al 1996;Brooks et al 1997;Lai et al 2000;Kubovcakova et al 2004;Gallara et al 2004;Lai et al 2005), require some experience to produce reliably reproducible results.…”

confidence: 99%

Expression Levels of mRNAs for Catecholamine Biosynthetic Enzymes as Markers of Acute Response to Contusion Stress during the Early Postmortem Period

Takahashi

2008

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

Various stresses can be followed by sudden unexpected deaths, and autopsies sometimes fail to identify pathological findings that determine the cause of death. Pathologists occasionally explain such deaths as being due to overstimulation of sympathoadrenal systems, but postmortem assessment of antemortem sympathoadrenal activity has not been established. An animal model of weight injuries was used to quantify sympathoadrenal response to contusion stress, which is common in forensic fields. A weight was dropped from a given height onto the right dorsal limb of each anesthetized rat, with a control group and three stress groups (n = 4, each): 1000 g-80 cm, 1000 g-40 cm, and 500 g-40 cm. To explore the postmortem changes, we also included ten groups comprised of control and 1000 g-80 cm groups, whose tissues were harvested during 12 hours after euthanasia. Real-time quantitative polymerase-chain reaction was performed to quantify relative expression levels of mRNAs for catecholamine biosynthetic enzymes in the adrenals and the anterocervical ganglia: tyrosine hydroxylase (TH), dopamine betahydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT). The expression levels of all target mRNAs in the adrenals increased with the intensity of impact (TH, p < 0.0005; DBH and PNMT, p < 0.005), and particularly, TH mRNA level exhibited near-stepwise elevation ( p < 0.05). In contrast, no significant differences were detected in the anterocervical ganglia. Moreover, these mRNA levels in the adrenals decreased with increasing postmortem interval length. Thus, TH mRNA level may be a good marker of sympathoadrenal response to contusion stress during the early postmortem period. forensic medicine; sudden unexpected death; sympathoadrenal response to contusion stress; catecholamine; real-time quantitative polymerase chain reaction.Tohoku

show abstract

Stress-triggered activation of gene expression in catecholaminergic systems: dynamics of transcriptional events

Cited by 262 publications

References 76 publications

Elevated Concentrations of CRF in the Locus Coeruleus of Depressed Subjects

Elevated Concentrations of CRF in the Locus Coeruleus of Depressed Subjects

T-type channels-secretion coupling: evidence for a fast low-threshold exocytosis

Expression Levels of mRNAs for Catecholamine Biosynthetic Enzymes as Markers of Acute Response to Contusion Stress during the Early Postmortem Period

Contact Info

Product

Resources

About