Stretching of red blood cells using an electro-optics trap

Haque, Md. Mozzammel; Moisescu, Mihaela G.; Valkai, Sándor; Dér, András; Savopol, Tudor

doi:10.1364/boe.6.000118

Cited by 22 publications

(13 citation statements)

References 25 publications

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“…The cell stretching technologies are emerging inexhaustibly as well as [23][24][25]. Considering the intuitiveness and easiness, our stretching method is shown in Fig.…”

Section: Measurement Methodsmentioning

confidence: 99%

Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers

Song¹,

Liu²,

Zhang³

et al. 2016

Biomed. Opt. Express

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Abstract:The elasticity of red cell membrane is a critical physiological index for the activity of RBC. Study of the inherent mechanism for RBCs membrane elasticity transformation is attention-getting all along. This paper proposes an optimized measurement method of erythrocytes membrane shear modulus incorporating acousto-optic deflector (AOD) scanning optical tweezers system. By use of this method, both membrane shear moduli and sizes of RBCs with different in vitro times were determined. The experimental results reveal that the RBCs membrane elasticity and size decline with in vitro time extension. In addition, semi quantitative measurements of S-nitrosothiol content in blood using fluorescent spectrometry during in vitro storage show that RBCs membrane elasticity change is positively associated with the S-nitrosylation level of blood. The analysis considered that the diminished activity of the nitric oxide synthase makes the S-nitrosylation of in vitro blood weaker gradually. The main reason for worse elasticity of the in vitro RBCs is that S-nitrosylation effect of spectrin fades. These results will provide a guideline for further study of in vitro cells activity and other clinical applications. References and links1. N. Hamasaki and M. Yamamoto, "Red blood cell function and blood storage," Vox Sang. 79(4), 191-197 (2000). 2. M. Whirter, J. Liam, H. Noguchi, and G. Gompper, "Ordering and arrangement of deformed red blood cells in flow through microcapillaries," New J. Phys. 14(8), 6709-6717 (2012). 3. M. Musielak, "Red blood cell-deformability measurement: review of techniques," Clin. Hemorheol. Microcirc.42(1), 47-64 (2009). 4. S. Svetina, G. Kokot, T. S. Kebe, B. Žekš, and R. E. Waugh, "A novel strain energy relationship for red blood cell membrane skeleton based on spectrin stiffness and its application to micropipette deformation," Biomech. Model. Mechanobiol. 15(3), 745-758 (2016). 5. G. J. Streekstra, J. G. G. Dobbe, and A. G. Hoekstra, "Quantification of the fraction poorly deformable red blood cells using ektacytometry," Opt. Express 18(13), 14173-14182 (2010). 6. L. Dintenfass, "Internal viscosity of the red cell and a blood viscosity equation," Nature 219(5157), 956-958 (1968). 649-652 (1986). 37. Z. Peng, X. Li, I. V. Pivkin, M. Dao, G. E. Karniadakis, and S. Suresh, "Lipid bilayer and cytoskeletal interactions in a red blood cell," Proc. Natl. Acad. Sci. U.S.A. 110(33), 13356-13361 (2013). 38. M. Grau, P. Friederichs, S. Krehan, C. Koliamitra, F. Suhr, and W. Bloch, "Decrease in red blood cell deformability is associated with a reduction in RBC-NOS activation during storage," Clin. Hemorheol. Microcirc. 60(2), 215-229 (2015).

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“…The cell stretching technologies are emerging inexhaustibly as well as [23][24][25]. Considering the intuitiveness and easiness, our stretching method is shown in Fig.…”

Section: Measurement Methodsmentioning

confidence: 99%

Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers

Song¹,

Liu²,

Zhang³

et al. 2016

Biomed. Opt. Express

View full text Add to dashboard Cite

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“…Several techniques have been adopted for studying mechanical properties of RBCs. Many of them are based on the design of specific microfluidic channels [12][13][14][15], and on various electrical/optical techniques, such as atomic force microscopy [16], dielectrophoresis [17], and optical trapping techniques [18,19] or acoustic-based approaches [20].…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic Red Blood Cells Deformation by Quantitative Phase Microscopy and Zernike Polynomials

et al. 2019

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Red Blood Cells (RBCs) deformability is an important parameter for evaluating their health status. Its quantification is performed through the measurement of erythrocyte's shape stiffness when subjected to external stimuli. Here, we exploit the hydrodynamic deformation of RBCs in microfluidic channels to quantify the shape variations through quantitative phase imaging by digital holography. In particular, two main processing steps have been employed, i.e., the morphological analysis based on quantitative phase variations and a new way to monitor the entire cell's deformation, based on modeling RBCs as a micro-lenses array. In fact, taking advantage of the RBC lens behavior, it is possible to correlate optical aberrations generated by the mechanical stimulus to the entire membrane deformation itself, through a numerical analysis based on Zernike polynomials. We provide a proof of principle of how to use Zernike analysis for characterizing RBCs' deformability under hydrodynamic stress. We demonstrate that new optical parameters of RBCs can be measured and analyzed, thus opening the route to the exploitation of the bio-lens model as a biomechanical marker of RBCs.

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“…11. Both due to their potential use as diagnostic markers, and their abundance and availability, single cells from blood have received particular interest, [12][13][14][15][16] and are often used as first cell type for a new methodology, see e.g. Ref.…”

Section: Introductionmentioning

confidence: 99%