Striking effects of melatonin on secondary metabolites produced by callus culture of rosemary (Rosmarinus officinalis L.)

Coşkun, Yasemin; Duran, Ragbet Ezgi; Kılıç, Semra

doi:10.1007/s11240-019-01605-7

Cited by 44 publications

(17 citation statements)

References 25 publications

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“…Several studies have reported the prominent role of melatonin in plant growth and development. It provides the necessary protection for the plant cell from the negative impact of various abiotic stresses [18][19][20][21][22] by stimulating gene expression [23] leading to the activation of the antioxidant enzymatic and nonenzymatic systems [24][25][26][27][28][29]. Melatonin is believed to have key role in increasing the mitochondrial efficiency of the electron transport chain.…”

Section: Introductionmentioning

confidence: 99%

Positive Response of Hyoscyamus pusillus Callus Cultures to Exogenous Melatonin on Biochemical Traits and Secondary Metabolites under Drought Conditions

Neamah

Jdayea

2022

International Journal of Agronomy

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There is increased attention from specialists toward producing natural compounds from plant tissues through the combined use of elicitors. The present study investigated the effects of melatonin treatment in normal and drought stress conditions for sustainable production of biomass and secondary antioxidant metabolites in a Hyoscyamus pusillus four-month-old callus cultures. For this purpose, we used multiple concentrations of melatonin (0, 0.5, 1.0, 1.5, and 2.0 mg l−1) to assess its ability to improve growth, physiological, and biochemical properties of H. pusillus. There was two drought stress levels (0 and 30 g l−1) of polyethylene glycol (PEG). It was harvested in 28 days. The results showed a significant decrease with an increase in the concentration of PEG treatment in growth and physiological traits compared to the results of those samples when treated with melatonin. Results showed an increase in oxidative stress in tissue treated with PEG due to a significant increase in the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA). The decreased oxidative stress was associated with an increased antioxidant enzyme activity superoxide dismutase (SOD), and catalase (CAT) in the samples was treated by melatonin, which resulted in increased membrane stability index (MSI) and enhanced growth traits under the PEG treatment compared to the control. By reducing the activity of phenylalanine ammonia-lyase (PAL) and peroxidase (POX), moisture tightening increased the production of phenolic compounds (PC) and flavonoid compounds (FC) in callus cultures, and high concentrations of melatonin were combined with them to improve their production. Therefore, it can be asserted that a moderate treatment with melatonin is more suitable under water stress conditions to produce secondary compounds from H. pusillus.

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Section: Introductionmentioning

confidence: 99%

Positive Response of Hyoscyamus pusillus Callus Cultures to Exogenous Melatonin on Biochemical Traits and Secondary Metabolites under Drought Conditions

Neamah

Jdayea

2022

International Journal of Agronomy

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“…In addition to the stress tolerance-inducing elicitors, stress tolerance-enhancer compounds, such as melatonin, have been applied as elicitors to increase the accumulation of in vitro bioactive components of medicinal plants (Coskun et al 2019;Duran et al 2019). Other stress tolerance-enhancer additives, such as gibberelin inhibitors, ethylene inhibitors, osmoprotectants, antioxidant activators, ROS scavengers, and detoxi cation activators (Niazian and Shariatpanahi 2020), can potentially be used in combination with stress-inducing elicitors (PEG, NaCl, heavy metal ions) to increase the in vitro production of plants bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Niazian

Howyzeh

Sadat-Noori

2021

Plant Cell Tiss Organ Cult

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Bioactive compounds of medicinal plants have a wide range of applications in pharmaceutical, food and other industries. In vitro culture systems have great potential for sustainable production of bioactive compounds of medicinal plants. In the present study, the individual and combined effects of a stress tolerance-inducing (salicylic acid) and a stress-inducing elicitor (polyethylene glycol) were evaluated on regeneration e ciency, antioxidants activity and phytochemical pro le of in vitro shoot cultures of ajowan. Different concentrations of salicylic acid (SA) (0, 10, 20, 40, 80 µM) and polyethylene glycol (PEG 6000) (0, 1, 2, 5%) were added to the shoot regeneration Murashige and Skoog medium containing Kin (1.5 mg/L) and NAA (0.25 mg/L) plant growth regulators. Salicylic acid reduced the adverse effect of PEG treatment on number of regenerated shoots and in vitro rooting. The activities of catalase, superoxide dismutase, and peroxidase enzymatic antioxidants were signi cantly increased in SA + PEG treated plants. The gas chromatography-mass spectrometry (GC-MS)-pro ling revealed quantitative and qualitative phytochemical differences between control and SA + PEG treated plants. The greatest means of p-cymene and thymol bioactive compounds were obtained from in vitro shoots treated with 5% PEG + 40 µM SA. The inter-simple sequence repeats (ISSR) markers proved the genetic stability of in vitro regenerated plants. The presented protocol is useful for large-scale sustainable production of secondary metabolites (SMs) of medicinal plants. The same strategy (stress tolerance-inducing elicitor + stress-inducing elicitor) is applicable to increase valuable SMs in other production systems such as hydroponic, greenhouse and eld conditions.

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“…The studies about rosemary tissue culture which were conducted by Coskun (2019) and research group express that melatonin induced seconder metabolite content as elicitor. Another investigations were also determined that antioxidant enzymes become functional with phytomelatonin application (Li et al, 2012).…”

Section: Antioxidant Activity Of Rosemary Plantsmentioning

confidence: 99%

Studies on Antimicrobial, Antifungal and Antioxidant Properties of Rosemary: A Review

Erkan

Aşcı

2020

Turkish JAF Sci.Tech.

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Rosemary has played an important role from past to present and has antimicrobial, antifungal and antioxidant properties. With these features, it is used in many sectors, especially food and pharmacy. Rosemary essential oils have a positive effect on biological activity. In addition, this oil prevents lipid oxidation on foods, providing a long and fresh shelf life. Due to the high antioxidant properties of rosemary, it eliminates the harmful effect of reactive oxygen species. Since the main components of rosemary such as 1,8-cineol, camphor, α-pinene, carnosic acid, and carnosol are being antimicrobial and antifungal, it is effective against many pathogens. With this review, it is aimed to provide comprehensive information on the biological activities of rosemary and its extracts to shed light on future research.

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Striking effects of melatonin on secondary metabolites produced by callus culture of rosemary (Rosmarinus officinalis L.)

Cited by 44 publications

References 25 publications

Positive Response of Hyoscyamus pusillus Callus Cultures to Exogenous Melatonin on Biochemical Traits and Secondary Metabolites under Drought Conditions

Positive Response of Hyoscyamus pusillus Callus Cultures to Exogenous Melatonin on Biochemical Traits and Secondary Metabolites under Drought Conditions

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Studies on Antimicrobial, Antifungal and Antioxidant Properties of Rosemary: A Review

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